TIM-1/KIM-1/HAVCR: Novus Biologicals

AF1817

TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

100 μg

AF1817

HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Exogenous biological renal support improved renal IRI and decreased mortality and serum Cr, BUN levels in old IRI mice. (A) Survival curves for the old IRI mice at 72 hours. (B) Cr levels in the old mice. (C) BUN levels in the old mice. (D) Representative photographs of kidney sections from the old mice stained with periodic acid–Schiff (400× magnification). (E) Renal tubular injury score. (F) The levels of Kim1 in kidney extracts from the old mice, as measured by western blotting. Gels were performed under the same experimental conditions. (G) Quantitative analyses of the band densities of Kim1 expression. Values are presented as means ± SDs. ▲P < 0.05, ▲▲P < 0.01 vs. O: sham; *P < 0.05, **P < 0.01 vs. O: IRI. BUN, blood urea nitrogen Cr, serum creatinine; SD, standard deviation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30978173), licensed under a CC-BY license. Not internally tested by R&D Systems.

Changes in tubular injury and repair after MSC transplantation via the subcapsular and parenchymal routes. A Representative immunofluorescence staining of PCNA (red) and LTL (marker of proximal tubule; green) in the groups after IRI. B Quantitative analysis of PCNA-positive cells in the LTL+ proximal tubules in the groups after IRI. ***p < 0.001, ANOVA corrected with the Bonferroni coefficient. n = 5 per group. C Representative immunofluorescence staining of KIM-1 (red) in the groups after IRI. D Quantitative analysis showing the KIM-1-positive areas in the groups after IRI. ***p < 0.001, ANOVA corrected with the Bonferroni coefficient. n = 5 per group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35337372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Elucidation of kidney injury markers and MAPK signaling following rhabdomyolysis. Mice were administered 7.5 ml/kg body weight of 50% glycerol in water and harvested after 1 or 3 days. Kidneys were analyzed for the expression of (A) kidney injury molecule-1 (KIM-1), (B) neutrophil gelatinase associated lipocalin (NGAL), and (C) ferritin heavy chain (FtH) by real-time PCR analysis. Each experiment was performed at least three independent times. Results were normalized to GAPDH and presented as mean ± SEM. *p < 0.05 vs. baseline control; #p < 0.05 vs. FtHfl/fl. (D) Kidneys were analyzed for the expression of 4-hydroxynonenal, NGAL, KIM-1, FtH, p-ERK, total ERK, p-JNK, and total JNK by Western blot analysis. Membranes were stripped and re-probed for GAPDH to demonstrate equal loading. (E) Expression of the indicated proteins in the kidneys was analyzed by densitometry, normalized to GAPDH (or total JNK or total ERK) and expressed as mean ± SEM. *p < 0.05 vs. baseline control; #p < 0.05 vs. FtHfl/fl. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36160140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Downregulation of KLF10 was correlated with cell proliferation and the outcome of cisplatin-induced AKI in vivo. A Flowchart detailing cisplatin treatment regime in C57BL/6 J mice. n = 6 per group. B Representative H&E staining image of kidney sections from the model. C Representative immunofluorescence staining image of Ki67 in the model and counts of positive cells per 40x field (objective lens). D Renal dysfunction was determined in cisplatin (20 mg/kg, ip) treated mouse model. Serum creatinine (SCr) was measured in sera. Degree of renal injury was determined in the model through relative mRNA levels of KIM1, NGAL and IGFBP7 in mouse kidney tissues. Degree of renal cellular proliferation was determined in the model through relative mRNA levels of PCNA, FOXM1 and Ki67 in mice kidney tissues. E Representative immunoblot analysis of KLF10, KIM1 and PCNA in the model. ACTIN served as the standard. F Regression analysis was undertaken to determine correlation between relative protein level of KLF10 in mice kidney tissues and SCr. Relative protein level of KLF10 was examined and normalized by Fiji. G–I Representative immunofluorescence staining image and rate of positive nuclear of KLF10 in the model. *p < 0.05, **p < 0.01, ***p < 0.001, ns no significantly difference vs control group at the same experimental conditions. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36878898), licensed under a CC-BY license. Not internally tested by R&D Systems.

DMM inhibited UUO-induced renal fibrosis and injury. (A) The schematic of the experimental design. (B) The succinate content in PBS- and DMM-treated UUO kidneys (n = 5). (C) Immunoblots and (D) quantification of Col I and alpha SMA in UUO kidneys from mice treated with or without DMM (n = 3). (E) Representative Masson staining and quantification of UUO kidneys from mice treated with or without DMM (n = 5). (F) Immunoblots and (G) quantification of KIM1 and cleaved caspase-3 in UUO kidneys from mice treated with or without DMM (n = 3). The results represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40556756), licensed under a CC-BY license. Not internally tested by R&D Systems.

1817-TM) was serially diluted and captured by Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse TIM‑1/KIM‑1/HAVCR (Catalog # <a class=

1817-TM) was serially diluted and captured by Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1817U-100UG
Alexa Fluor 405	AF1817V-100UG
Alexa Fluor 488	AF1817G-100UG
Alexa Fluor 532	AF1817X-100UG
Alexa Fluor 594	AF1817T-100UG
Alexa Fluor 647	AF1817R-100UG
Alexa Fluor 700	AF1817N-100UG
Alexa Fluor 750	AF1817S-100UG
Alexa Fluor Plus 405	AF1817AFP405-100UG
Alexa Fluor Plus 488	AF1817AFP488-100UG
Alexa Fluor Plus 555	AF1817AFP555-100UG
Alexa Fluor Plus 594	AF1817AFP594-100UG
Alexa Fluor Plus 647	AF1817AFP647-100UG
Alexa Fluor Plus 680	AF1817AFP680-100UG
Allophycocyanin	AF1817A
Biotin	BAF1817
CoraFluor 1	AF1817CL1
DyLight 350	AF1817D
DyLight 405	AF1817E
DyLight 488	AF1817K
DyLight 550	AF1817L
DyLight 594	AF1817M
DyLight 650	AF1817W
DyLight 680	AF1817Y
DyLight 755	AF1817Z
FITC	AF1817F
HRP	AF1817H
Janelia Fluor 525	AF1817JF525
Janelia Fluor 549	AF1817I
Janelia Fluor 646	AF1817J
Janelia Fluor 669	AF1817JF669
PE	AF1817P
PerCP	AF1817C
mFluor Violet 450 SE	AF1817MFV450
mFluor Violet 500 SE	AF1817MFV500
mFluor Violet 610 SE	AF1817MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1817-SP

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NBP1-76701

TIM-1/KIM-1/HAVCR Antibody - BSA Free

(38 Publications)

0.1 mg

NBP1-76701

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Independent Antibodies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), overnightincubation at 4C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Genetic Strategies Validation. </b>Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. Antibodies: 3809 (8 ug/mL), 1 hincubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 u of lysates per lane.Antibodies: TIM-1 (8 u/mL), overnight incubation at 4; C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Validation of TIM-1/KIM-1/HAVCR in Human Uterus Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1/KIM-1/HAVCR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.

KIM1 mediates the cell entry of SARS-CoV-2-RBD. (A) Representative images and quantitative data of cell surface attachment of SARS-CoV-2-RBD in wild-type (WT) and KIM1 knockout (KIM1-KO) HK-2 cells. Scale bar, 20 μm. ***P < 0.001. (B) Representative images and quantitative data of cell surface attachment of SARS-CoV-2-RBD in HEK293T cells. Scale bar, 20 μm. OE KIM1, overexpression of KIM1. **P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33493263), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76701AF350
Alexa Fluor 405	NBP1-76701AF405
Alexa Fluor 488	NBP1-76701AF488
Alexa Fluor 532	NBP1-76701AF532
Alexa Fluor 594	NBP1-76701AF594
Alexa Fluor 647	NBP1-76701AF647
Alexa Fluor 700	NBP1-76701AF700
Alexa Fluor 750	NBP1-76701AF750
Alexa Fluor Plus 488	NBP1-76701AFP488
Alexa Fluor Plus 555	NBP1-76701AFP555
Alexa Fluor Plus 594	NBP1-76701AFP594
Alexa Fluor Plus 647	NBP1-76701AFP647
Alexa Fluor Plus 680	NBP1-76701AFP680
Allophycocyanin	NBP1-76701APC
Biotin	NBP1-76701B
CoraFluor 1	NBP1-76701CL1
DyLight 350	NBP1-76701UV
DyLight 405	NBP1-76701V
DyLight 488	NBP1-76701G
DyLight 550	NBP1-76701R
DyLight 594	NBP1-76701DL594
DyLight 650	NBP1-76701C
DyLight 680	NBP1-76701FR
DyLight 755	NBP1-76701IR
FITC	NBP1-76701F
HRP	NBP1-76701H
Janelia Fluor 525	NBP1-76701JF525
Janelia Fluor 549	NBP1-76701JF549
Janelia Fluor 646	NBP1-76701JF646
Janelia Fluor 669	NBP1-76701JF669
PE	NBP1-76701PE
PerCP	NBP1-76701PCP
mFluor Violet 450 SE	NBP1-76701MFV450
mFluor Violet 500 SE	NBP1-76701MFV500
mFluor Violet 610 SE	NBP1-76701MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76701SS

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AF1750

TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

(42 Publications)

100 μg

AF1750

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AF1750) at 1ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tubules. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of human kidney were probed for TIM1 mRNA (ACD RNAScope Probe, catalog #452148; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human TIM1 polyclonal antibody (R&D Systems catalog # <a class=

AF1750) at 1ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tubules. " class="big_thumb" />

1750-TM) was serially diluted and captured by Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB17502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human TIM‑1/KIM‑1/HAVCR His-tag (Catalog # <a class=

1750-TM) was serially diluted and captured by Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB17502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1750-TM) was serially diluted and captured by Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB17502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1750-TM) was serially diluted and captured by Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB17502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1750U-100UG
Alexa Fluor 405	AF1750V-100UG
Alexa Fluor 488	AF1750G-100UG
Alexa Fluor 532	AF1750X-100UG
Alexa Fluor 594	AF1750T-100UG
Alexa Fluor 647	AF1750R-100UG
Alexa Fluor 700	AF1750N-100UG
Alexa Fluor 750	AF1750S-100UG
Alexa Fluor Plus 405	AF1750AFP405-100UG
Alexa Fluor Plus 488	AF1750AFP488-100UG
Alexa Fluor Plus 555	AF1750AFP555-100UG
Alexa Fluor Plus 594	AF1750AFP594-100UG
Alexa Fluor Plus 647	AF1750AFP647-100UG
Alexa Fluor Plus 680	AF1750AFP680-100UG
Allophycocyanin	AF1750A
Biotin	BAF1750
CoraFluor 1	AF1750CL1
DyLight 350	AF1750D
DyLight 405	AF1750E
DyLight 488	AF1750K
DyLight 550	AF1750L
DyLight 594	AF1750M
DyLight 650	AF1750W
DyLight 680	AF1750Y
DyLight 755	AF1750Z
FITC	AF1750F
HRP	AF1750H
Janelia Fluor 525	AF1750JF525
Janelia Fluor 549	AF1750I
Janelia Fluor 646	AF1750J
Janelia Fluor 669	AF1750JF669
PE	AF1750P
PerCP	AF1750C
mFluor Violet 450 SE	AF1750MFV450
mFluor Violet 500 SE	AF1750MFV500
mFluor Violet 610 SE	AF1750MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1750-SP

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AF3689

TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

(43 Publications)

100 μg

AF3689

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

TIM-1/KIM-1/HAVCR was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Rat TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="TIM-1/KIM-1/HAVCR was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Rat TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Immunohistochemical analysis of kidneys in LETO and OLETF rats.Representative immunohistochemical images of glomerular, tubular and tubulointerstitial lesions in each group, 12 weeks after starting to feed a Paigen diet. Scale bar, 100 μm. KIM-1, kidney injury molecule-1; MRP8, myeloid-related protein 8; NOX2, NADPH oxidase 2; OPN, osteopontin; TLR4, toll-like receptor 4; TNF-alpha , tumor necrosis factor-alpha . Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0143979), licensed under a CC-BY license. Not internally tested by R&D Systems.

3689-TM) was serially diluted and captured by Mouse Anti-Rat TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB3689) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Rat TIM‑1/KIM‑1/HAVCR (Catalog # <a class=

3689-TM) was serially diluted and captured by Mouse Anti-Rat TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB3689) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3689) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3689U-100UG
Alexa Fluor 405	AF3689V-100UG
Alexa Fluor 488	AF3689G-100UG
Alexa Fluor 532	AF3689X-100UG
Alexa Fluor 594	AF3689T-100UG
Alexa Fluor 647	AF3689R-100UG
Alexa Fluor 700	AF3689N-100UG
Alexa Fluor 750	AF3689S-100UG
Alexa Fluor Plus 405	AF3689AFP405-100UG
Alexa Fluor Plus 488	AF3689AFP488-100UG
Alexa Fluor Plus 555	AF3689AFP555-100UG
Alexa Fluor Plus 594	AF3689AFP594-100UG
Alexa Fluor Plus 647	AF3689AFP647-100UG
Alexa Fluor Plus 680	AF3689AFP680-100UG
Allophycocyanin	AF3689A
Biotin	BAF3689
CoraFluor 1	AF3689CL1
DyLight 350	AF3689D
DyLight 405	AF3689E
DyLight 488	AF3689K
DyLight 550	AF3689L
DyLight 594	AF3689M
DyLight 650	AF3689W
DyLight 680	AF3689Y
DyLight 755	AF3689Z
FITC	AF3689F
HRP	AF3689H
Janelia Fluor 525	AF3689JF525
Janelia Fluor 549	AF3689I
Janelia Fluor 646	AF3689J
Janelia Fluor 669	AF3689JF669
PE	AF3689P
PerCP	AF3689C
mFluor Violet 450 SE	AF3689MFV450
mFluor Violet 500 SE	AF3689MFV500
mFluor Violet 610 SE	AF3689MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3689-SP

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DKM100

Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

(45 Publications)

1 Kit

DKM100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	SKM100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	SKM100

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MAB1817

TIM-1/KIM-1/HAVCR Antibody (222414) [Unconjugated]

(27 Publications)

100 μg

MAB1817

MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " alt=" HEK293 cells transfected with Mouse TIM-1 were stained with Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

1817-TM) was serially diluted and captured by Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1817-TM) was serially diluted and captured by Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1817U-100UG
Alexa Fluor 405	FAB1817V-100UG
Alexa Fluor 488	FAB1817G-100UG
Alexa Fluor 532	FAB1817X-100UG
Alexa Fluor 594	FAB1817T-100UG
Alexa Fluor 647	FAB1817R-100UG
Alexa Fluor 700	FAB1817N-100UG
Alexa Fluor 750	FAB1817S-100UG
Alexa Fluor Plus 405	FAB1817AFP405-100UG
Alexa Fluor Plus 488	FAB1817AFP488-100UG
Alexa Fluor Plus 555	FAB1817AFP555-100UG
Alexa Fluor Plus 594	FAB1817AFP594-100UG
Alexa Fluor Plus 647	FAB1817AFP647-100UG
Alexa Fluor Plus 680	FAB1817AFP680-100UG
Allophycocyanin	FAB1817A
Biotin	FAB1817B
CoraFluor 1	FAB1817CL1
DyLight 350	FAB1817D
DyLight 405	FAB1817E
DyLight 488	FAB1817K
DyLight 550	FAB1817L
DyLight 594	FAB1817M
DyLight 650	FAB1817W
DyLight 680	FAB1817Y
DyLight 755	FAB1817Z
FITC	FAB1817F
HRP	FAB1817H
Janelia Fluor 525	FAB1817JF525
Janelia Fluor 549	FAB1817I
Janelia Fluor 646	FAB1817J
Janelia Fluor 669	FAB1817JF669
PE	FAB1817P
PerCP	FAB1817C
mFluor Violet 450 SE	FAB1817MFV450
mFluor Violet 500 SE	FAB1817MFV500
mFluor Violet 610 SE	FAB1817MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1817A
Carrier Free	MAB1817-SP
Carrier Free	MAB1817-500

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MAB1750

TIM-1/KIM-1/HAVCR Antibody (219211) [Unconjugated]

(24 Publications)

100 μg

MAB1750

MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of human CD4<SUP>+</SUP>cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3e Monoclonal Antibody (<a class=

MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human CD4⁺cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3e Monoclonal Antibody (MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_lightbox">

<b>Biological Strategies Validation. </b>(A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM-1/ KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Huh‑7 human hepatoma cell line were stained with Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750, filled histogram) or isotype control antibody (Catalog # <a class=

MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1750U-100UG
Alexa Fluor 405	FAB1750V-100UG
Alexa Fluor 532	FAB1750X
Alexa Fluor 594	FAB1750T-100UG
Alexa Fluor 647	FAB1750R-100UG
Alexa Fluor 700	FAB1750N-100UG
Alexa Fluor 750	FAB1750S-100UG
Alexa Fluor Plus 405	FAB1750AFP405-100UG
Alexa Fluor Plus 488	FAB1750AFP488-100UG
Alexa Fluor Plus 555	FAB1750AFP555-100UG
Alexa Fluor Plus 594	FAB1750AFP594-100UG
Alexa Fluor Plus 647	FAB1750AFP647-100UG
Alexa Fluor Plus 680	FAB1750AFP680-100UG
Allophycocyanin	FAB1750A
Allophycocyanin/Cy7	FAB1750APCCY7
Biotin	FAB1750B
CoraFluor 1	FAB1750CL1
DyLight 350	FAB1750D
DyLight 405	FAB1750E
DyLight 488	FAB1750K
DyLight 550	FAB1750L
DyLight 594	FAB1750M
DyLight 650	FAB1750W
DyLight 680	FAB1750Y
DyLight 755	FAB1750Z
FITC	FAB1750F
HRP	FAB1750H
Janelia Fluor 525	FAB1750JF525
Janelia Fluor 549	FAB1750I
Janelia Fluor 646	FAB1750J
Janelia Fluor 669	FAB1750JF669
PE/Atto594	FAB1750PEATT594
PE/Cy5.5	FAB1750PECY55
PE/Cy7	FAB1750PECY7
PerCP	FAB1750C
mFluor Violet 450 SE	FAB1750MFV450
mFluor Violet 500 SE	FAB1750MFV500
mFluor Violet 610 SE	FAB1750MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1750-SP
Carrier Free	MAB1750-500
Carrier Free	FAB1750V-100UG
Carrier Free	FAB1750U-100UG
Carrier Free	FAB1750T-100UG
Carrier Free	FAB1750S-100UG
Carrier Free	FAB1750R-100UG
Carrier Free	FAB1750N-100UG

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MKM100

Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

(26 Publications)

1 Kit

MKM100

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NB100-56421

TIM-1/KIM-1/HAVCR Antibody - BSA Free

(3 Publications)

0.1 mg

NB100-56421

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5283A

Alt Catalog# IMG-5283A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56421AF350
Alexa Fluor 405	NB100-56421AF405
Alexa Fluor 488	NB100-56421AF488
Alexa Fluor 532	NB100-56421AF532
Alexa Fluor 594	NB100-56421AF594
Alexa Fluor 647	NB100-56421AF647
Alexa Fluor 700	NB100-56421AF700
Alexa Fluor 750	NB100-56421AF750
Alexa Fluor Plus 405	NB100-56421AFP405
Alexa Fluor Plus 488	NB100-56421AFP488
Alexa Fluor Plus 555	NB100-56421AFP555
Alexa Fluor Plus 594	NB100-56421AFP594
Alexa Fluor Plus 647	NB100-56421AFP647
Alexa Fluor Plus 680	NB100-56421AFP680
Allophycocyanin	NB100-56421APC
Biotin	NB100-56421B
CoraFluor 1	NB100-56421CL1
DyLight 350	NB100-56421UV
DyLight 405	NB100-56421V
DyLight 488	NB100-56421G
DyLight 550	NB100-56421R
DyLight 594	NB100-56421DL594
DyLight 650	NB100-56421C
DyLight 680	NB100-56421FR
DyLight 755	NB100-56421IR
FITC	NB100-56421F
HRP	NB100-56421H
Janelia Fluor 525	NB100-56421JF525
Janelia Fluor 549	NB100-56421JF549
Janelia Fluor 646	NB100-56421JF646
Janelia Fluor 669	NB100-56421JF669
PE	NB100-56421PE
PerCP	NB100-56421PCP
mFluor Violet 450 SE	NB100-56421MFV450
mFluor Violet 500 SE	NB100-56421MFV500
mFluor Violet 610 SE	NB100-56421MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56421SS

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RKM100

Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

(15 Publications)

1 Kit

RKM100

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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DY3689

Rat TIM-1/KIM-1/HAVCR DuoSet ELISA, 15 Plate

(5 Publications)

1 Kit (for 15 Plates)

DY3689

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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NBP3-42314

Porcine TIM-1/KIM-1/HAVCR ELISA Kit (Colorimetric)

1 Kit

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

Applications:

Standard Curve:

31.2 - 2000 pg/mL (example only; lot dependent)

Sensitivity:

13.9 pg/mL (example only; lot dependent)

Sample Type:

Tissue homogenates, Cell lysates and other biological fluids

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Havcr1 Products By Gene Name

TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

TIM-1/KIM-1/HAVCR Antibody - BSA Free

TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

TIM-1/KIM-1/HAVCR Antibody [Unconjugated]

Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

TIM-1/KIM-1/HAVCR Antibody (222414) [Unconjugated]

TIM-1/KIM-1/HAVCR Antibody (219211) [Unconjugated]

Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

TIM-1/KIM-1/HAVCR Antibody - BSA Free

Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

Rat TIM-1/KIM-1/HAVCR DuoSet ELISA, 15 Plate

Porcine TIM-1/KIM-1/HAVCR ELISA Kit (Colorimetric)