Recombinant Rat Lipocalin-2/NGAL Protein, CF Summary
Details of Functionality
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. Recombinant Rat Lipocalin‑2/NGAL can bind >1.0 µM of Fe(DHBA)3, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived rat Lipocalin-2/NGAL protein Gln21-Asn198, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Rat Lipocalin-2/NGAL Protein, CF
25 kDa alpha-2-microglobulin-related subunit of MMP-9
lipocalin 2 (oncogene 24p3)
migration-stimulating factor inhibitor
neutrophil gelatinase-associated lipocalin
Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll‑like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin‑2 can alter the invasive and metastatic behavior of Ras‑transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E‑cadherin expression, via effects on the Ras‑MAPK signaling pathway (6). In the kidney, Lipocalin‑2‑mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).
Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
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