Recombinant Rat IL-7 Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat IL-7 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED₅₀ for this effect is 0.1-0.6 ng/mL.
• Source: E. coli-derived rat IL-7 protein
  Asp26-Ile154, with an N-terminal Met
• Accession #: NP_037242
• N-terminal Sequence: Starts at Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il7
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-7 Protein, CF

• IL7
• IL-7
• IL-7interleukin-7
• interleukin 7
• Lymphopoietin-1
• PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Rat IL‑7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide. Mature rat IL‑7 shares approximately 88% aa sequence identity with mouse IL‑7 and 58‑60% with human, equine, bovine, ovine, porcine, feline and canine IL‑7. Human and mouse IL‑7 exhibit cross‑species activity (2, 3). IL‑7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL‑7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL‑7 signals through a complex of the IL‑7 Receptor alpha subunit (IL‑7 R alpha , also known as CD127) with the common gamma chain ( gamma _c) (1). The gamma _c is also a subunit of the receptors for IL‑2, ‑4, ‑9, ‑15, and ‑21 (1). IL‑7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive naïve and memory T cells, but undergoes IL‑7‑mediated down‑regulation and shedding during antigen‑driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6‑11). IL‑7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl‑2 (9‑11). It is required for optimal T cell‑dendritic cell interaction (6). IL‑7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL‑7 activation of IL‑7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL‑7 functions in both mouse and human pro‑B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
4. Namen, A.E. et al. (1988) Nature 333:571.
5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
6. Saini, M. et al. (2009) Blood 113:5793.
7. Park, J.H. et al. (2004) Immunity 21:289.
8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
15. Nodland, S.E. et al. (2011) Blood 118:2116.

Bioinformatics

• Gene Symbol: Il7
• Uniprot:
  • NP_037242(Rat)