Recombinant Rat IL-23 Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat IL-23 Protein Summary

Details of Functionality
Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.1-0.5 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat IL-23 protein
Rat IL-23 p40
(Met23 - Ser335)
Accession # NP_072133
IGSGSSRGGSGSGGSGGGGSK Rat IL-23 p19
(Leu20 - Ala196)
Accession # NP_569094
8-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
58.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
66 kDa, reducing conditions
Publications
Read Publication using
3136-RL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-23 Protein

  • IL-23 p19/IL-12 p40
  • IL23
  • IL-23
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL-23p19/IL-12p40
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. The rat p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. The mature rat p19 protein shares 88%, 78%, 76%, 75%, 71%, and 70% aa sequence identity with mouse, human, canine, equine, guinea pig, and bovine, respectively. Activated macrophages and dendritic cells express p19 and p40 concurrently to produce IL-23 (1, 4). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R) (3). IL-23 and IL-12 have overlapping but distinct biological activities. IL-12 drives development of Th1 cells and induces production of IFN-gamma by NK cells; IL-23 induces proliferation of Th17 cells and CD4+ memory T cells distinct from Th1 which produce IL-17, a potent proinflammatory cytokine (2). IL-23 also drives IL-17 production by NK cells and neutrophils (6). While both IL-12 and IL-23 pathways respond to infectious agents, the IL-23 - IL-17 immune pathway induces the earliest recruitment of neutrophils to the site of infection while the more classic host defense and cytotoxic response is stimulated by IL-12 (5). Dysregulation of the IL-23 - IL-17 immune pathway has a key role in organ-specific autoimmune inflammatory tissue destruction (2, 7).

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. McKenzie, B.S. et al. (2006) Trends Immunol. 27:17.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Cua, D.J. et al. (2003) Nature 421:744.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  6. Langrish, C.L. et al. (2004) Immunol. Rev. 202:96.
  7. Murphy, C.A. et al. (2003) J. Exp. Med. 198:1951.

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Publications for IL-23A/IL-23 P19 (3136-RL)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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