Recombinant Rat GFR alpha-1 Fc Chimera Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Binding Activity, Bioactivity

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Catalog# & Formulation Size Price

Recombinant Rat GFR alpha-1 Fc Chimera Protein Summary

Details of Functionality
Measured by its ability to confer GDNF responsiveness to PC‑12 rat adrenal pheochromocytoma cells using a neurite outgrowth assay. Treanor, J. et al. (1996) Nature 392:80. The ED50 for this effect is approximately 0.3 µg/mL in the presence of 25 ng/mL of Recombinant Rat GDNF (Catalog # 512-GF). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GDNF (Catalog # 512-GF) at 1 μg/mL binds Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent KD <5 nM.
Source
Mouse myeloma cell line, NS0-derived rat GFR alpha-1/GDNF R alpha-1 protein
Rat GFR alpha -1
(Asp25-Leu445)
Accession # Q62997
CSIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp25
Protein/Peptide Type
Recombinant Proteins
Gene
Gfra1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
74 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95 kDa, reducing conditions
Publications
Read Publications using
560-GR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat GFR alpha-1 Fc Chimera Protein

  • DKFZp313E1012
  • DKFZp686J0156
  • GDNF family receptor alpha 1
  • GDNF family receptor alpha-1
  • GDNF R alpha-1
  • GDNF receptor alpha-1
  • GDNFRAGFR-ALPHA-1
  • GDNFR-alpha-1
  • GDNFRMGC23045
  • GFR alpha1
  • GFR alpha-1
  • GFRA1
  • GFRa-1
  • GFR-alpha-1
  • Glial cell line-derived neurotrophic factor receptor alpha
  • GPI-linked anchor protein
  • PI-linked cell-surface accessory protein
  • RET ligand 1
  • RET1L
  • RETL1FLJ10561
  • TGF-beta related neurotrophic factor receptor 1
  • TGF-beta-related neurotrophic factor receptor 1
  • TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF‑ beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine‑rich glycosyl-phosphatidylinositol (GPI)‑linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

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560-GR
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Applications: Binding Activity, Bioactivity

Publications for GFR alpha-1/GDNF R alpha-1 (560-GR)(10)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Rabbit.

We have publications tested in 2 applications: Bioassay, ELISA development.


Filter By Application
Bioassay
(9)
ELISA development
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Rat
(4)
Chicken
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 560-GR Applications Species
Jiangkai Lin Curcumin inhibits glial scar formation by suppressing astrocyte-induced inflammation and fibrosis in vitro and in vivo Brain Res., 2016;0(0):. 2016 [PMID: 27865778] (Bioassay, Rat) Bioassay Rat
Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence Nature, 2016;535(7612):440-3. 2016 [PMID: 27409807] (Bioassay, Mouse) Bioassay Mouse
Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature, 2014;514(7520):98-101. 2014 [PMID: 25079320] (Bioassay, Mouse) Bioassay Mouse
Sakuraba, Juichi, Shintani, Takafumi, Tani, Sachiko, Noda, Masaharu Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases. J Biol Chem, 2013;288(32):23421-31. 2013 [PMID: 23814054] (Bioassay, Rat) Bioassay Rat
Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci, 2013;54(0):30-43. 2013 [PMID: 23262364] (Bioassay, Rat) Bioassay Rat
Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. FASEB J., 2011;25(0):2604-14. 2011 [PMID: 21525489] (Bioassay, Rabbit) Bioassay Rabbit
Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005;44(7):2545-54. 2005 [PMID: 15709767] (ELISA development, Rat) ELISA development Rat
Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells. J. Cell Biol., 2004;167(5):945-52. 2004 [PMID: 15569713] (Bioassay, Mouse) Bioassay Mouse
Cerchia L, Libri D, Carlomagno MS, de Franciscis V The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret. Biochem. J., 2003;372(0):897-903. 2003 [PMID: 12630912] (Bioassay, Human) Bioassay Human
Peterziel H, Unsicker K, Krieglstein K TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane. J. Cell Biol., 2002;159(1):157-67. 2002 [PMID: 12370242] (Bioassay, Chicken) Bioassay Chicken

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Bioinformatics

Gene Symbol Gfra1
Uniprot