Recombinant Rat GFR alpha-1 Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Binding Activity, Bioactivity
• Format: Carrier-Free

Recombinant Rat GFR alpha-1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to confer GDNF responsiveness to PC‑12 rat adrenal pheochromocytoma cells using a neurite outgrowth assay. Treanor, J. et al. (1996) Nature 392:80. The ED₅₀ for this effect is approximately 0.3 µg/mL in the presence of 25 ng/mL of Recombinant Rat GDNF (Catalog # 512-GF). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GDNF (Catalog # 512-GF) at 1 μg/mL binds Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent K_D <5 nM.
• Source: Mouse myeloma cell line, NS0-derived rat GFR alpha-1/GDNF R alpha-1 protein
  

  Rat GFR alpha -1 (Asp25-Leu445) Accession # Q62997	CSIEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: Q62997
• N-terminal Sequence: Asp25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gfra1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• Theoretical MW: 74 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat GFR alpha-1 Fc Chimera Protein, CF

• DKFZp313E1012
• DKFZp686J0156
• GDNF family receptor alpha 1
• GDNF family receptor alpha-1
• GDNF R alpha-1
• GDNF receptor alpha-1
• GDNFRAGFR-ALPHA-1
• GDNFR-alpha-1
• GDNFRMGC23045
• GFR alpha1
• GFR alpha-1
• GFRA1
• GFRa-1
• GFR-alpha-1
• Glial cell line-derived neurotrophic factor receptor alpha
• GPI-linked anchor protein
• PI-linked cell-surface accessory protein
• RET ligand 1
• RET1L
• RETL1FLJ10561
• TGF-beta related neurotrophic factor receptor 1
• TGF-beta-related neurotrophic factor receptor 1
• TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF‑ beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine‑rich glycosyl-phosphatidylinositol (GPI)‑linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Publications for GFR alpha-1/GDNF R alpha-1 (560-GR/CF)(14)

Publications using 560-GR/CF

• A Boyer, X Zhang, A Levasseur, N Abou Nader, G St-Jean, MC Nagano, D Boerboom Constitutive activation of CTNNB1 results in a loss of spermatogonial stem cell activity in mice PLoS ONE, 2021-05-20;16(5):e0251911. 2021-05-20 [PMID: 34015032] (Bioassay, Transgenic Mouse)
• LL Porokuokka, HT Virtanen, J Lindén, Y Sidorova, T Danilova, M Lindahl, M Saarma, JO Andressoo Gfra1 under-expression causes Hirschsprung&#039;s disease and associated enterocolitis in mice Cell Mol Gastroenterol Hepatol, 2018-12-27;0(0):. 2018-12-27 [PMID: 30594740] (Bioassay, Mouse)
• T Shindo, S Doi, A Nakashima, K Sasaki, K Arihiro, T Masaki TGF-?1 promotes expression of fibrosis-related genes through the induction of histone variant H3.3 and histone chaperone HIRA Sci Rep, 2018-09-19;8(1):14060. 2018-09-19 [PMID: 30232404] (Bioassay, Rat)
• K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis Biol. Reprod., 2017-03-01;96(3):707-719. 2017-03-01 [PMID: 28339678] (Cell Culture, Rat)
• Jiangkai Lin Curcumin inhibits glial scar formation by suppressing astrocyte-induced inflammation and fibrosis in vitro and in vivo Brain Res., 2016-11-16;0(0):. 2016-11-16 [PMID: 27865778] (Bioassay, Rat)
• Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence Nature, 2016-07-13;535(7612):440-3. 2016-07-13 [PMID: 27409807] (Bioassay, Mouse)
• Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature, 2014-07-27;514(7520):98-101. 2014-07-27 [PMID: 25079320] (Bioassay, Mouse)
• Sakuraba , Juichi, Shintani , Takafumi, Tani , Sachiko, Noda , Masaharu Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases. J Biol Chem, 2013-06-28;288(32):23421-31. 2013-06-28 [PMID: 23814054] (Bioassay, Rat)
• Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci, 2012-12-20;54(0):30-43. 2012-12-20 [PMID: 23262364] (Bioassay, Rat)
• Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. FASEB J., 2011-04-27;25(0):2604-14. 2011-04-27 [PMID: 21525489] (Bioassay, Rabbit)
• Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005-02-22;44(7):2545-54. 2005-02-22 [PMID: 15709767] (Bioassay, ELISA Developmet, Surface Plasmon Resonance, Rat)
• Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells. J. Cell Biol., 2004-11-29;167(5):945-52. 2004-11-29 [PMID: 15569713] (Bioassay, Mouse)
• Cerchia L, Libri D, Carlomagno MS, de Franciscis V The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret. Biochem. J., 2003-06-15;372(0):897-903. 2003-06-15 [PMID: 12630912] (Bioassay, Human)
• Peterziel H, Unsicker K, Krieglstein K TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane. J. Cell Biol., 2002-10-07;159(1):157-67. 2002-10-07 [PMID: 12370242] (Bioassay, Chicken)

Bioinformatics

• Gene Symbol: Gfra1
• Uniprot:
  • Q62997()