Recombinant Rat GDNF Protein, CF

Recombinant Rat GDNF(Catalog # 512-GF/CF) promotes the survival of chick embryonic dorsal root ganglia neurons. The ED50 for this effect is 1-3 ng/mL.

1 μg/lane of Recombinant Rat GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19 kDa and 33 kDa, respectively.

• Reactivity: Rt
• Applications: Binding Activity, Bioactivity
• Format: Carrier-Free

Recombinant Rat GDNF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED 50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha -1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha -1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR) at 1 µg/mL binds Recombinant Rat GDNF with an apparent K d <4 nM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein Ser78-Ile211
• Accession #: Q07731
• N-terminal Sequence: Ser78
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gdnf
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• Theoretical MW: 15 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat GDNF Protein, CF

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Publications for GDNF (512-GF/CF)(42)

Publications using 512-GF/CF

• M Caillaud, ME Le Dréan, A De-Guilhem, C Le Berre-S, J Montnach, S Nedellec, G Loussouarn, V Paillé, M Neunlist, H Boudin A functional network of highly pure enteric neurons in a dish Frontiers in Neuroscience, 2023-01-06;16(0):1062253. 2023-01-06 [PMID: 36685225] (Bioassay, Rat)
• G D'Este, M Stazi, S Negro, A Megighian, F Lista, O Rossetto, C Montecucco, M Rigoni, M Pirazzini Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration International Journal of Molecular Sciences, 2022-01-21;23(3):. 2022-01-21 [PMID: 35163106] (Bioassay, Rat)
• J Zhao, P Lu, C Wan, Y Huang, M Cui, X Yang, Y Hu, Y Zheng, J Dong, M Wang, S Zhang, Z Liu, S Bian, X Wang, R Wang, S Ren, D Wang, Z Yao, G Chang, F Tang, XY Zhao Cell-fate transition and determination analysis of mouse male germ cells throughout development Nature Communications, 2021-11-25;12(1):6839. 2021-11-25 [PMID: 34824237] (Bioassay, Mouse)
• Y Xie, W Zhao, Z Zuo Glial cell-derived neurotrophic factor decrease may mediate learning, memory and behavior impairments in rats after neonatal surgery Brain research bulletin, 2021-10-30;0(0):. 2021-10-30 [PMID: 34728231] (In Vivo, Rat)
• F Cardoso, RGJ Klein Wolt, C Godinho-Si, RG Domingues, H Ribeiro, JA da Silva, I Mahú, AI Domingos, H Veiga-Fern Neuro-mesenchymal units control ILC2 and obesity via a brain-adipose circuit Nature, 2021-08-18;0(0):. 2021-08-18 [PMID: 34408322] (Bioassay, Mouse)
• S Takaku, M Tsukamoto, N Niimi, H Yako, K Sango Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro International Journal of Molecular Sciences, 2021-03-15;22(6):. 2021-03-15 [PMID: 33804063] (Bioassay, Rat)
• CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3 Cellular and Molecular Gastroenterology and Hepatology, 2021-01-11;0(0):. 2021-01-11 [PMID: 33444816] (Bioassay, Mouse)
• LS VandenBosc, SG Wohl, MS Wilken, M Hooper, C Finkbeiner, K Cox, L Chipman, TA Reh Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in M�ller Glial regenerative potential Sci Rep, 2020-08-12;10(1):13615. 2020-08-12 [PMID: 32788677] (Bioassay, Mouse)
• A Javadi, S Mokhtari, SF Moraveji, FA Sayahpour, M Farzaneh, H Gourabi, F Esfandiari Short Time Exposure to Low Concentration of Zinc Oxide Nanoparticles Up-regulates Self-renewal and Spermatogenesis-Related Gene Expression Int. J. Biochem. Cell Biol., 2020-08-06;127(0):105822. 2020-08-06 [PMID: 32771442] (Bioassay, Mouse)
• BE Kerman, S Genoud, B Kurt Vatan, AM Denli, SG Ghosh, X Xu, GW Yeo, JB Aimone, FH Gage Motoneuron expression profiling identifies an association between an axonal splice variant of HDGF-related protein 3 and peripheral myelination J. Biol. Chem., 2020-07-09;0(0):. 2020-07-09 [PMID: 32647008] (Cell Culture, Rat)
• JE Hartung, MS Gold GCaMP as an indirect measure of electrical activity in rat trigeminal ganglion neurons Cell Calcium, 2020-05-30;89(0):102225. 2020-05-30 [PMID: 32505783] (Bioassay, Rat)
• S Kuroda, Y Miyagawa, Y Sato, M Yamamoto, K Adachi, H Kinoh, WF Goins, JB Cohen, JC Glorioso, N Taniai, H Yoshida, T Okada Protocol Optimization for the Production of the Non-Cytotoxic J&amp;deltaNI5 HSV Vector Deficient in Expression of Immediately Early Genes Mol Ther Methods Clin Dev, 2020-03-17;17(0):612-621. 2020-03-17 [PMID: 32300608] (Bioassay, Rat)
• K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng Biological and RNA regulatory function of MOV10 in mammalian germ cells BMC Biol., 2019-05-14;17(1):39. 2019-05-14 [PMID: 31088452] (Bioassay, Mouse)
• C Wu, ME Watts, LL Rubin MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 2019-01-29;26(5):1143-1156.e5. 2019-01-29 [PMID: 30699345] (Bioassay, Mouse)
• Y Bai, C Zhu, M Feng, H Wei, L Li, X Tian, Z Zhao, S Liu, N Ma, X Zhang, R Shi, C Fu, Z Wu, S Zhang Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells Stem Cell Res Ther, 2018-07-18;9(1):200. 2018-07-18 [PMID: 30021628] (Bioassay, Porcine)
• KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018-05-22;9(6):596. 2018-05-22 [PMID: 29789529] (Bioassay, Mouse)
• JR Cabrera, AJ Charron, DA Leib Neuronal subtype determines HSV-1 Latency-Associated-Transcript (LAT) promoter activity during latency J. Virol., 2018-06-13;0(0):. 2018-06-13 [PMID: 29643250] (Cell Culture, Mouse)
• N Aoki, K Mochizuki, Y Matsui DNA methylation of the Fthl17 5&#039;-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells PLoS ONE, 2017-02-16;12(2):e0172219. 2017-02-16 [PMID: 28207785] (Bioassay, Mouse)
• In�Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells Cell Rep, 2016-12-06;17(10):2789-2804. 2016-12-06 [PMID: 27926879] (Bioassay, Mouse)
• A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016-05-25;0(0):. 2016-05-25 [PMID: 27226316] (Bioassay, Rat)
• A Suzuki, M Hirasaki, T Hishida, J Wu, D Okamura, A Ueda, M Nishimoto, Y Nakachi, Y Mizuno, Y Okazaki, Y Matsui, JC Izpisua Be, A Okuda Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells Nat Commun, 2016-03-30;7(0):11056. 2016-03-30 [PMID: 27025988] (Bioassay, Mouse)
• Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE, 2015-06-17;10(6):e0129944. 2015-06-17 [PMID: 26083449] (Bioassay, Mouse)
• Lim , Jung Jin, Seol , Dong Won, Choi , Kyung He, Shin , Dong Hyu, Kim , Hyung Jo, Song , Seung-Hu, Lee , Dong Ryu Spermatogonial stem cell enrichment using simple grafting of testis and in vitro cultivation. Sci Rep, 2014-08-01;4(0):5923. 2014-08-01 [PMID: 25080919] (Bioassay, Mouse)
• Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature, 2014-07-27;514(7520):98-101. 2014-07-27 [PMID: 25079320] (Bioassay, Mouse)
• Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J, 2014-06-11;33(14):1582-98. 2014-06-11 [PMID: 24920579] (Bioassay, Mouse)
• Lim J, Shim M, Lee J, Lee D Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells. PLoS ONE, 2014-04-01;9(4):e90454. 2014-04-01 [PMID: 24690677] (Bioassay, Human)
• Matsumoto , Shinji, Fujii , Shinsuke, Sato , Akira, Ibuka , Souji, Kagawa , Yoshinor, Ishii , Masaru, Kikuchi , Akira A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J, 2014-02-20;33(7):702-18. 2014-02-20 [PMID: 24562386] (Bioassay, Mouse)
• Sakuraba , Juichi, Shintani , Takafumi, Tani , Sachiko, Noda , Masaharu Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases. J Biol Chem, 2013-06-28;288(32):23421-31. 2013-06-28 [PMID: 23814054] (Bioassay, Rat)
• Dovere , Lisa, Fera , Stefania, Grasso , Margheri, Lamberti , Dante, Gargioli , Cesare, Muciaccia , Barbara, Lustri , Anna Mar, Stefanini , Mario, Vicini , Elena The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells. PLoS ONE, 2013-04-22;8(4):e59431. 2013-04-22 [PMID: 23613711] (Bioassay, Mouse)
• Hong , Weimin C, Amara , Susan G Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons. FASEB J, 2013-04-23;27(8):2995-3007. 2013-04-23 [PMID: 23612789] (Bioassay, Rat)
• Gui T, Sun Y, Gai Z, Shimokado A, Muragaki Y, Zhou G The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling. Dev Biol, 2013-03-26;377(2):415-27. 2013-03-26 [PMID: 23537899] (Bioassay, Mouse)
• Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013-03-20;33(12):5399-410. 2013-03-20 [PMID: 23516305] (Bioassay, Mouse)
• Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci, 2012-12-20;54(0):30-43. 2012-12-20 [PMID: 23262364] (Bioassay, Rat)
• Non-viral gene therapy for GDNF production in RCS rat: the crucial role of the plasmid dose. Gene Ther., 2011-10-13;19(9):886-98. 2011-10-13 [PMID: 21993171] (ELISA (Standard), Western Blot)
• Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos. Development, 2010-03-01;137(5):693-704. 2010-03-01 [PMID: 20147374] (Bioassay, Mouse)
• Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T Long-term culture of male germline stem cells from hamster testes. Biol. Reprod., 2007-12-19;78(4):611-7. 2007-12-19 [PMID: 18094355] (Bioassay, Hamster)
• He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation. Stem Cells, 2007-10-25;26(1):266-78. 2007-10-25 [PMID: 17962702] (Bioassay, Mouse)
• Garbayo E, Ansorena E, Lanciego JL, Aymerich MS, Blanco-Prieto MJ Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor. Int J Pharm, 2007-04-06;344(1):9-15. 2007-04-06 [PMID: 17499462] (Bioassay, Western Blot, Rat)
• Hamra FK, Chapman KM, Nguyen D, Garbers DL Identification of neuregulin as a factor required for formation of aligned spermatogonia. J. Biol. Chem., 2006-11-10;282(1):721-30. 2006-11-10 [PMID: 17098736] (Bioassay, Rat)
• Murata T, Tsuboi M, Hikita K, Kaneda N Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J. Biol. Chem., 2006-06-13;281(32):22503-16. 2006-06-13 [PMID: 16772303] (Bioassay, Mouse)
• Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury. J. Clin. Invest., 2005-08-18;115(9):2393-401. 2005-08-18 [PMID: 16110328] (In Vivo, Rat)
• Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005-02-22;44(7):2545-54. 2005-02-22 [PMID: 15709767] (Bioassay, ELISA Developmet, Rat)

Bioinformatics

• Gene Symbol: Gdnf
• Uniprot:
  • Q07731()