Recombinant Rat GDNF Protein

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1 μg/lane of Recombinant Rat GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19 kDa and 33 kDa, respectively.
Recombinant Rat GDNF (Catalog # 512‑GF) promotes the survival of chick embryonic dorsal root ganglia neurons. The ED50 for this effect is 1-3 ng/mL.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Binding Activity, Bioactivity

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Catalog# & Formulation Size Price

Recombinant Rat GDNF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha ‑1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR) at 1 µg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein
Ser78-Ile211
Accession #
N-terminal Sequence
Ser78
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Gdnf
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
512-GF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat GDNF Protein

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • HFB1-GDNF
  • hGDNF
  • HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

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Publications for GDNF (512-GF)(24)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Porcine.

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, WB Ctrl.


Filter By Application
Bioassay
(21)
ELISA Standard
(1)
In Vivo
(1)
WB Ctrl
(1)
All Applications
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Human
(1)
Mouse
(12)
Rat
(7)
Hamster
(1)
N/A
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using 512-GF Applications Species
Y Bai, C Zhu, M Feng, H Wei, L Li, X Tian, Z Zhao, S Liu, N Ma, X Zhang, R Shi, C Fu, Z Wu, S Zhang Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells Stem Cell Res Ther, 2018;9(1):200. 2018 [PMID: 30021628] (Bioassay, Porcine) Bioassay Porcine
KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018;9(6):596. 2018 [PMID: 29789529] (Bioassay, Mouse) Bioassay Mouse
N Aoki, K Mochizuki, Y Matsui DNA methylation of the Fthl17 5&#039;-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells PLoS ONE, 2017;12(2):e0172219. 2017 [PMID: 28207785] (Bioassay, Mouse) Bioassay Mouse
In�Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells Cell Rep, 2016;17(10):2789-2804. 2016 [PMID: 27926879] (Bioassay, Mouse) Bioassay Mouse
A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016;0(0):. 2016 [PMID: 27226316] (Bioassay, Rat) Bioassay Rat
A Suzuki, M Hirasaki, T Hishida, J Wu, D Okamura, A Ueda, M Nishimoto, Y Nakachi, Y Mizuno, Y Okazaki, Y Matsui, JC Izpisua Be, A Okuda Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells Nat Commun, 2016;7(0):11056. 2016 [PMID: 27025988] (Bioassay, Mouse) Bioassay Mouse
Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE, 2015;10(6):e0129944. 2015 [PMID: 26083449] (Bioassay, Mouse) Bioassay Mouse
Lim, Jung Jin, Seol, Dong Won, Choi, Kyung He, Shin, Dong Hyu, Kim, Hyung Jo, Song, Seung-Hu, Lee, Dong Ryu Spermatogonial stem cell enrichment using simple grafting of testis and in vitro cultivation. Sci Rep, 2014;4(0):5923. 2014 [PMID: 25080919] (Bioassay, Mouse) Bioassay Mouse
Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature, 2014;514(7520):98-101. 2014 [PMID: 25079320] (Bioassay, Mouse) Bioassay Mouse
Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J, 2014;33(14):1582-98. 2014 [PMID: 24920579] (Bioassay, Mouse) Bioassay Mouse
Show All 24 Publications.

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Blogs on GDNF.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

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Bioinformatics

Gene Symbol Gdnf
Entrez
Uniprot