Recombinant Rat GDNF Protein


1 μg/lane of Recombinant Rat GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19 kDa and 33 kDa, respectively.
Recombinant Rat GDNF (Catalog # 512‑GF) promotes the survival of chick embryonic dorsal root ganglia neurons. The ED50 for this effect is 1-3 ng/mL.

Product Details

Reactivity RtSpecies Glossary
Applications Binding Activity, Bioactivity

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Recombinant Rat GDNF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha ‑1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR) at 1 µg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
512-GF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat GDNF Protein

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • HSCR3


Glial Cell Line-derived Neurotrophic Factor (GDNF) is a discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

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Publications for GDNF (512-GF)(29)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Hamster, N/A, Porcine.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using 512-GF Applications Species
BE Kerman, S Genoud, B Kurt Vatan, AM Denli, SG Ghosh, X Xu, GW Yeo, JB Aimone, FH Gage Motoneuron expression profiling identifies an association between an axonal splice variant of HDGF-related protein 3 and peripheral myelination J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32647008] (Cell Culture, Rat) Cell Culture Rat
S Kuroda, Y Miyagawa, Y Sato, M Yamamoto, K Adachi, H Kinoh, WF Goins, JB Cohen, JC Glorioso, N Taniai, H Yoshida, T Okada Protocol Optimization for the Production of the Non-Cytotoxic J&amp;deltaNI5 HSV Vector Deficient in Expression of Immediately Early Genes Mol Ther Methods Clin Dev, 2020;17(0):612-621. 2020 [PMID: 32300608] (Bioassay, Rat) Bioassay Rat
K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng Biological and RNA regulatory function of MOV10 in mammalian germ cells BMC Biol., 2019;17(1):39. 2019 [PMID: 31088452] (Bioassay, Mouse) Bioassay Mouse
C Wu, ME Watts, LL Rubin MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 2019;26(5):1143-1156.e5. 2019 [PMID: 30699345] (Bioassay, Mouse) Bioassay Mouse
Y Bai, C Zhu, M Feng, H Wei, L Li, X Tian, Z Zhao, S Liu, N Ma, X Zhang, R Shi, C Fu, Z Wu, S Zhang Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells Stem Cell Res Ther, 2018;9(1):200. 2018 [PMID: 30021628] (Bioassay, Porcine) Bioassay Porcine
KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018;9(6):596. 2018 [PMID: 29789529] (Bioassay, Mouse) Bioassay Mouse
JR Cabrera, AJ Charron, DA Leib Neuronal subtype determines HSV-1 Latency-Associated-Transcript (LAT) promoter activity during latency J. Virol., 2018;0(0):. 2018 [PMID: 29643250] (Cell Culture, Mouse) Cell Culture Mouse
N Aoki, K Mochizuki, Y Matsui DNA methylation of the Fthl17 5&#039;-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells PLoS ONE, 2017;12(2):e0172219. 2017 [PMID: 28207785] (Bioassay, Mouse) Bioassay Mouse
In�Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells Cell Rep, 2016;17(10):2789-2804. 2016 [PMID: 27926879] (Bioassay, Mouse) Bioassay Mouse
A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016;0(0):. 2016 [PMID: 27226316] (Bioassay, Rat) Bioassay Rat
Show All 29 Publications.

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The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

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Gene Symbol Gdnf