Recombinant Mouse TSG-6 Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse TSG-6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmTSG-6 can bind biotinylated Hyaluronan with an estimated KD <1 nM.
Mouse myeloma cell line, NS0-derived mouse TSG-6 protein
Trp18-Leu275, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
39 kDa, reducing conditions
Read Publications using
2326-TS in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TSG-6 Protein, CF

  • Hyaluronate-binding protein
  • TNF alpha-induced protein 6
  • TNF-stimulated gene 6 protein
  • TSG6
  • TSG-6
  • TSG-6tumor necrosis factor alpha-inducible protein 6
  • TSG6tumor necrosis factor-inducible gene 6 protein
  • Tumor necrosis factor alpha-induced protein 6
  • tumor necrosis factor, alpha-induced protein 6
  • tumor necrosis factor-stimulated gene-6 protein


TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35 - 39 kDa group A member of the LINK-Module superfamily of proteins (1 - 4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36 - 129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135 - 246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80 - 85 kDa protein subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Fulop, C. et al. (1997) Gene 202:95.
  6. Fulop, C. et al. (2003) Development 130:2253.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

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Publications for TSG-6 (2326-TS)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: Bioassay, In Vivo, Western Blot.

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In Vivo
Western Blot
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Showing Publications 1 - 8 of 8.
Publications using 2326-TS Applications Species
MGA Broeren, I Di Ceglie, MB Bennink, PLEM van Lent, WB van den Be, MI Koenders, EN Blaney Dav, PM van der Kr, FAJ van de Loo Treatment of collagenase-induced osteoarthritis with a viral vector encoding TSG-6 results in ectopic bone formation PeerJ, 2018;6(0):e4771. 2018 [PMID: 29868252] (Bioassay, Mouse) Bioassay Mouse
Endotoxin free hyaluronan and hyaluronan fragments do not stimulate TNF-?, interleukin-12 or upregulate co-stimulatory molecules in dendritic cells or macrophages Sci Rep, 2016;6(0):36928. 2016 [PMID: 27869206] (Bioassay, Mouse) Bioassay Mouse
Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V Hyaluronan Rafts on Airway Epithelial Cells. J Biol Chem, 2016;291(3):1448-55. 2016 [PMID: 26601955] (Western Blot, Mouse) Western Blot Mouse
Torihashi S, Ho M, Kawakubo Y, Komatsu K, Nagai M, Hirayama Y, Kawabata Y, Takenaka-Ninagawa N, Wanachewin O, Zhuo L, Kimata K Acute and temporal expression of tumor necrosis factor (TNF)-alpha-stimulated gene 6 product, TSG6, in mesenchymal stem cells creates microenvironments required for their successful transplantation into muscle tissue. J Biol Chem, 2015;290(37):22771-81. 2015 [PMID: 26178374]
Fujita R, Tamai K, Aikawa E, Nimura K, Ishino S, Kikuchi Y, Kaneda Y Endogenous mesenchymal stromal cells in bone marrow are required to preserve muscle function in mdx mice. Stem Cells, 2015;33(3):962-75. 2015 [PMID: 25408548] (In Vivo, Mouse) In Vivo Mouse
Liu Y, Zhang R, Yan K, Chen F, Huang W, Lv B, Sun C, Xu L, Li F, Jiang X Mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory responses of BV2 microglial cells through TSG-6. J Neuroinflammation, 2014;11(0):135. 2014 [PMID: 25088370] (Bioassay, Mouse) Bioassay Mouse
Wang N, Li Q, Zhang L, Lin H, Hu J, Li D, Shi S, Cui S, Zhou J, Ji J, Wan J, Cai G, Chen X Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6. PLoS ONE, 2012;7(8):e43768. 2012 [PMID: 22912904] (In Vivo, Rat) In Vivo Rat
Tuo J, Cao X, Shen D, Wang Y, Zhang J, Oh JY, Prockop DJ, Chan CC Anti-inflammatory recombinant TSG-6 stabilizes the progression of focal retinal degeneration in a murine model. J Neuroinflammation, 2012;9(0):59. 2012 [PMID: 22452753] (In Vivo, Mouse) In Vivo Mouse

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Gene Symbol Tnfaip6