Recombinant Mouse TSG-6 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse TSG-6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmTSG-6 can bind biotinylated Hyaluronan with an estimated KD <1 nM.
Source
Mouse myeloma cell line, NS0-derived mouse TSG-6 protein
Trp18-Leu275, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Trp18
Protein/Peptide Type
Recombinant Proteins
Gene
Tnfaip6
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
39 kDa, reducing conditions
Publications
Read Publications using
2326-TS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TSG-6 Protein, CF

  • Hyaluronate-binding protein
  • TNF alpha-induced protein 6
  • TNFAIP6
  • TNF-stimulated gene 6 protein
  • TSG6
  • TSG-6
  • TSG-6tumor necrosis factor alpha-inducible protein 6
  • TSG6tumor necrosis factor-inducible gene 6 protein
  • Tumor necrosis factor alpha-induced protein 6
  • tumor necrosis factor, alpha-induced protein 6
  • tumor necrosis factor-stimulated gene-6 protein

Background

TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35 - 39 kDa group A member of the LINK-Module superfamily of proteins (1 - 4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36 - 129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135 - 246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80 - 85 kDa protein subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Fulop, C. et al. (1997) Gene 202:95.
  6. Fulop, C. et al. (2003) Development 130:2253.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

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Publications for TSG-6 (2326-TS)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: Bioassay, In Vivo, Western Blot.


Filter By Application
Bioassay
(3)
In Vivo
(3)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 2326-TS Applications Species
MGA Broeren, I Di Ceglie, MB Bennink, PLEM van Lent, WB van den Be, MI Koenders, EN Blaney Dav, PM van der Kr, FAJ van de Loo Treatment of collagenase-induced osteoarthritis with a viral vector encoding TSG-6 results in ectopic bone formation PeerJ, 2018;6(0):e4771. 2018 [PMID: 29868252] (Bioassay, Mouse) Bioassay Mouse
Endotoxin free hyaluronan and hyaluronan fragments do not stimulate TNF-?, interleukin-12 or upregulate co-stimulatory molecules in dendritic cells or macrophages Sci Rep, 2016;6(0):36928. 2016 [PMID: 27869206] (Bioassay, Mouse) Bioassay Mouse
Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V Hyaluronan Rafts on Airway Epithelial Cells. J Biol Chem, 2016;291(3):1448-55. 2016 [PMID: 26601955] (Western Blot, Mouse) Western Blot Mouse
Torihashi S, Ho M, Kawakubo Y, Komatsu K, Nagai M, Hirayama Y, Kawabata Y, Takenaka-Ninagawa N, Wanachewin O, Zhuo L, Kimata K Acute and temporal expression of tumor necrosis factor (TNF)-alpha-stimulated gene 6 product, TSG6, in mesenchymal stem cells creates microenvironments required for their successful transplantation into muscle tissue. J Biol Chem, 2015;290(37):22771-81. 2015 [PMID: 26178374]
Fujita R, Tamai K, Aikawa E, Nimura K, Ishino S, Kikuchi Y, Kaneda Y Endogenous mesenchymal stromal cells in bone marrow are required to preserve muscle function in mdx mice. Stem Cells, 2015;33(3):962-75. 2015 [PMID: 25408548] (In Vivo, Mouse) In Vivo Mouse
Liu Y, Zhang R, Yan K, Chen F, Huang W, Lv B, Sun C, Xu L, Li F, Jiang X Mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory responses of BV2 microglial cells through TSG-6. J Neuroinflammation, 2014;11(0):135. 2014 [PMID: 25088370] (Bioassay, Mouse) Bioassay Mouse
Wang N, Li Q, Zhang L, Lin H, Hu J, Li D, Shi S, Cui S, Zhou J, Ji J, Wan J, Cai G, Chen X Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6. PLoS ONE, 2012;7(8):e43768. 2012 [PMID: 22912904] (In Vivo, Rat) In Vivo Rat
Tuo J, Cao X, Shen D, Wang Y, Zhang J, Oh JY, Prockop DJ, Chan CC Anti-inflammatory recombinant TSG-6 stabilizes the progression of focal retinal degeneration in a murine model. J Neuroinflammation, 2012;9(0):59. 2012 [PMID: 22452753] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol Tnfaip6
Uniprot