Recombinant Mouse TrkB Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse TrkB Protein Summary

Additional Information
His-tag
Details of Functionality
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.5-3.5 µg/mL in the presence  recombinant human BDNF and 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived mouse TrkB protein
Cys32-His429, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Cys32
Protein/Peptide Type
Recombinant Proteins
Gene
Ntrk2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
45.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-100 kDa, reducing conditions
Publications
Read Publications using
1494-TB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TrkB Protein

  • BDNF/NT-3 growth factors receptor
  • EC 2.7.10
  • EC 2.7.10.1
  • GP145-TrkB
  • Neurotrophic tyrosine kinase receptor type 2
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • TrkB tyrosine kinase
  • TrkB
  • trk-B
  • TRKBBDNF-tropomyosine receptor kinase B
  • tyrosine kinase receptor B

Background

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1-2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5-6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Nakagawara A. et al. (1995) Genomics. 25:538.
  6. Klein, R. et al. (1989) EMBO J. 8:3701.

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Publications for TrkB (1494-TB)(2)

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PTMs for TrkB (1494-TB)

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Blogs on TrkB.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Bioinformatics

Gene Symbol Ntrk2
Uniprot