Recombinant Mouse TLR3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TLR3 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED₅₀ for this effect is 2-12 µg/mL in the presence of 0.2 µg/mL of poly I:C.
• Source: Mouse myeloma cell line, NS0-derived mouse TLR3 protein
  Cys29-Leu705, with a C-terminal 6-His tag
• Accession #: Q99MB1
• N-terminal Sequence: Cys29
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tlr3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 77.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 114-127 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TLR3 Protein, CF

• CD283 antigen
• CD283
• TLR3
• toll-like receptor 3

Background

Mouse TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The mouse TLR3 cDNA encodes a 905 amino acid (aa) precursor that contains a 25 aa signal sequence, a 680 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine-rich repeats, and the cytoplasmic domain contains one
Toll/IL-1 receptor (TIR) domain. The ECD of mouse TLR3 shares 90%, 79%, and 77% aa sequence identity with the ECD of rat, human, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8-11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8), leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11-13). Mouse TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13-15), and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced on lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

1. Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
2. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
3. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
4. Applequist, S.E. et al. (2002) Int. Immunol. 14:1065.
5. Choe, J. et al. (2005) Science 309:581.
6. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10976.
7. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
8. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
9. Alexopoulou, L. et al. (2001) Nature 413:732.
10. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
11. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
12. Rudd, B.D. et al. (2005) J. Virol. 79:3350.
13. Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
14. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
15. Wang, T. et al. (2004) Nat. Med. 10:1366.

Bioinformatics

• Gene Symbol: Tlr3
• Uniprot:
  • Q99MB1()