Recombinant Mouse TGF-beta 1 Protein

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Recombinant mouse TGF-beta 1 (7666-MB) inhibits IL-4-dependent proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse TGF-beta 1 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse TGF-beta 1 protein
Ala279-Ser390
Accession #
N-terminal Sequence
Ala279
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tgfb1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
10.5 kDa, reducing conditions
Publications
Read Publications using
7666-MB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TGF-beta 1 Protein

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGF beta
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human or mouse TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature mouse TGF‑ beta 1 shares 99‑100% aa sequence identity with human, rat, equine, porcine, canine and bovine TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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7666-MB
Species: Mu
Applications: Bioactivity

Publications for TGF-beta 1 (7666-MB)(337)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, N/A, Transgenic Mouse.

We have publications tested in 8 applications: Bioassay, Cell Culture, ELISA Developmet, ELISA Standard, In Vivo, Stimulation, Surface Plasmon Resonance, Tissue Culture.


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Bioassay
(294)
Cell Culture
(30)
ELISA Developmet
(1)
ELISA Standard
(1)
In Vivo
(8)
Stimulation
(1)
Surface Plasmon Resonance
(1)
Tissue Culture
(1)
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Filter By Species
Human
(4)
Mouse
(322)
Rat
(10)
Canine
(1)
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(1)
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(6)
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Showing Publications 1 - 10 of 337. Show All 337 Publications.
Publications using 7666-MB Applications Species
Kweon, B;Kim, DU;Oh, JY;Park, SJ;Bae, GS; Catechin hydrate ameliorates cerulein?induced chronic pancreatitis via the inactivation of TGF??/Smad2 signaling Molecular medicine reports 2023-11-01 [PMID: 37732516] (Bioassay, Mouse) Bioassay Mouse
Wu, S;Zhang, X;Hu, C;Zhong, Y;Chen, J;Chong, WP; CD8+ T cells reduce neuroretina inflammation in mouse by regulating autoreactive Th1 and Th17 cells through IFN-? European journal of immunology 2023-09-09 [PMID: 37689974] (Bioassay, Mouse) Bioassay Mouse
van Os, BW;Vos, WG;Bosmans, LA;van Tiel, CM;Toom, MD;Beckers, L;Admiraal, M;Hoeksema, MA;de Winther, MP;Lutgens, E; CD40L modulates CD4+ T cell activation through receptor for activated C kinase 1 European journal of immunology 2023-09-08 [PMID: 37683186] (Bioassay, Mouse) Bioassay Mouse
Li, Y;Chen, Y;Xiao, X;Deng, S;Kuang, J;Wang, Y; CX3CL1 represses autophagy via CX3CR1/ CaMKII?/HDAC4/Rubicon axis and exacerbates chronic intermittent hypoxia induced Kupffer cell apoptosis Cellular signalling 2023-08-26 [PMID: 37640194] (Cell Culture, Mouse) Cell Culture Mouse
Santiago, JV;Natu, A;Ramelow, CC;Rayaprolu, S;Xiao, H;Kumar, V;Seyfried, NT;Rangaraju, S; Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles bioRxiv : the preprint server for biology 2023-07-29 [PMID: 37546899] (Mouse) Mouse
Chauvin, M;Meinsohn, MC;Dasari, S;May, P;Iyer, S;Nguyen, NMP;Oliva, E;Lucchini, Z;Nagykery, N;Kashiwagi, A;Mishra, R;Maser, R;Wells, J;Bult, CJ;Mitra, AK;Donahoe, PK;Pépin, D; Cancer-associated mesothelial cells are regulated by the anti-Müllerian hormone axis Cell reports 2023-07-13 [PMID: 37453057] (Human) Human
Goulart, A;Boko, MMM;Martins, NS;Gembre, AF;de Oliveira, RS;Palma-Albornoz, SP;Bertolini, T;Ribolla, PEM;Ramalho, LNZ;Fraga-Silva, TFC;Bonato, VLD; IL-22 Is Deleterious along with IL-17 in Allergic Asthma but Is Not Detrimental in the Comorbidity Asthma and Acute Pneumonia International journal of molecular sciences 2023-06-21 [PMID: 37445595] (Bioassay, Mouse, Transgenic Mouse) Bioassay Mouse, Transgenic Mouse
Vinod, N;Hwang, D;Fussell, SC;Owens, TC;Tofade, OC;Copling, S;Ramsey, JD;Rädler, PD;Atkins, HM;Livingston, EE;Ashley Ezzell, J;Papkov, MS;Yuan, H;Perou, CM;Kabanov, AV; Combination of Polymeric Micelle Formulation of TGF beta Receptor Inhibitors and Paclitaxel Produce Consistent Response Across Different Mouse Models of TNBC bioRxiv : the preprint server for biology 2023-06-14 [PMID: 37398150] (Bioassay, Mouse) Bioassay Mouse
Alber, AB;Marquez, HA;Ma, L;Kwong, G;Thapa, BR;Villacorta-Martin, C;Lindstrom-Vautrin, J;Bawa, P;Wang, F;Luo, Y;Ikonomou, L;Shi, W;Kotton, DN; Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme Nature communications 2023-06-13 [PMID: 37311756] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Suzuki, K;Kunisada, Y;Miyamura, N;Eikawa, S;Hurtado de Mendoza, T;Mose, ES;Lu, C;Kuroda, Y;Ruoslahti, E;Lowy, AM;Sugahara, KN; Tumor-resident regulatory T cells in pancreatic cancer express the ?v?5 integrin as a targetable activation marker bioRxiv : the preprint server for biology 2023-05-24 [PMID: 37292693] (Bioassay, Mouse) Bioassay Mouse
Show All 337 Publications.

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Blogs on TGF-beta 1.

TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta ...  Read full blog post.

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Bioinformatics

Gene Symbol Tgfb1
Uniprot