Recombinant Mouse TGF-beta 1 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse TGF-beta 1 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse TGF-beta 1 protein
Ala279-Ser390
Accession #
N-terminal Sequence
Ala279
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tgfb1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
10.5 kDa, reducing conditions
Publications
Read Publications using
7666-MB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TGF-beta 1 Protein

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human or mouse TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature mouse TGF‑ beta 1 shares 99‑100% aa sequence identity with human, rat, equine, porcine, canine and bovine TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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7666-MB
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Applications: Bioactivity

Publications for TGF-beta 1 (7666-MB)(192)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 6 applications: Bioassay, Cell Culture, ChIP, ELISA Developmet, In Vivo, Tissue Culture.


Filter By Application
Bioassay
(174)
Cell Culture
(9)
ChIP
(1)
ELISA Developmet
(1)
In Vivo
(4)
Tissue Culture
(1)
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Filter By Species
Human
(1)
Mouse
(184)
Rat
(4)
Canine
(1)
All Species
Showing Publications 1 - 10 of 192. Show All 192 Publications.
Publications using 7666-MB Applications Species
A Adhyatmika, L Beljaars, KSS Putri, H Habibie, CE Boorsma, C Reker-Smit, T Luangmonko, B Guney, A Haak, KA Mangnus, E Post, K Poelstra, K Ravnskjaer, P Olinga, BN Melgert Osteoprotegerin is More than a Possible Serum Marker in Liver Fibrosis: A Study into its Function in Human and Murine Liver Pharmaceutics, 2020;12(5):. 2020 [PMID: 32455750] (Tissue Culture, Mouse) Tissue Culture Mouse
LF Hu, J Feng, X Dai, Y Sun, M Xiong, L Lai, S Zhong, C Yi, G Chen, H Li, Q Yang, Q Kuang, T Long, J Zhan, T Tang, C Ge, J Tan, M Xu Oral flavonoid fisetin treatment protects against prolonged high-fat-diet-induced cardiac dysfunction by regulation of multicombined signaling J. Nutr. Biochem., 2019;77(0):108253. 2019 [PMID: 31835147] (Bioassay, Mouse) Bioassay Mouse
MG Kim, J Yang, YS Ko, HY Lee, SW Oh, WY Cho, SK Jo Impact of aging on transition of acute kidney injury to chronic kidney disease Sci Rep, 2019;9(1):18445. 2019 [PMID: 31804508] (Bioassay, Mouse) Bioassay Mouse
HG Lee, LK Kim, JM Choi NFAT-Specific Inhibition by dNP2-VIVITAmeliorates Autoimmune Encephalomyelitisby Regulation of Th1 and Th17 Mol Ther Methods Clin Dev, 2020;16(0):32-41. 2020 [PMID: 31737742] (Cell Culture, Mouse) Cell Culture Mouse
M Li, Y Zhao, D Qi, J He, D Wang Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17?cell response in mice Microb. Pathog., 2019;138(0):103826. 2019 [PMID: 31676364] (Cell Culture, Mouse) Cell Culture Mouse
P Kadam, E Ntemou, J Onofre, D Van Saen, E Goossens Does co-transplantation of mesenchymal and spermatogonial stem cells improve reproductive efficiency and safety in mice? Stem Cell Res Ther, 2019;10(1):310. 2019 [PMID: 31640769] (Bioassay, Mouse) Bioassay Mouse
W Zhou, Y Yang, C Mei, P Dong, S Mu, H Wu, Y Zhou, Y Zheng, F Guo, JQ Yang Inhibition of Rho-Kinase Downregulates Th17 Cells and Ameliorates Hepatic Fibrosis by Schistosoma japonicum Infection Cells, 2019;8(10):. 2019 [PMID: 31623153] (Cell Culture, Mouse) Cell Culture Mouse
AA Lombardi, AA Gibb, E Arif, DW Kolmetzky, D Tomar, TS Luongo, P Jadiya, EK Murray, PK Lorkiewicz, G Hajnóczky, E Murphy, ZP Arany, DP Kelly, KB Margulies, BG Hill, JW Elrod Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation Nat Commun, 2019;10(1):4509. 2019 [PMID: 31586055] (Cell Culture, Mouse) Cell Culture Mouse
G Liu, MA Cooley, AG Jarnicki, T Borghuis, PM Nair, G Tjin, AC Hsu, TJ Haw, M Fricker, CL Harrison, B Jones, NG Hansbro, PA Wark, JC Horvat, WS Argraves, BG Oliver, DA Knight, JK Burgess, PM Hansbro Fibulin-1c regulates transforming growth factor-beta activation in pulmonary tissue fibrosis JCI Insight, 2019;5(0):. 2019 [PMID: 31343988] (Bioassay, Mouse) Bioassay Mouse
C Lao, X Zhou, H Chen, F Wei, Z Huang, C Bai 5,6,7,8-Tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidine derivatives as inhibitors of full-length RORγt Bioorg. Chem., 2019;90(0):103077. 2019 [PMID: 31323598] (Cell Culture, Mouse) Cell Culture Mouse
Show All 192 Publications.

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TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta...  Read full blog post.

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Bioinformatics

Gene Symbol Tgfb1
Uniprot