Recombinant Mouse SIGIRR Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SIGIRR Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse SIGIRR protein
  

  Mouse SIGIRR (Met1 - His117) Accession # Q9JLZ8	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9JLZ8
• N-terminal Sequence: Met1
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sigirr
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 63 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SIGIRR Fc Chimera Protein, CF

• IL-1R8
• SIGIRR
• single Ig IL-1-related receptor
• Single Ig IL-1R-related molecule
• single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
• single immunoglobulin domain IL1R1 related
• Single immunoglobulin domain-containing IL1R-related protein
• TIR8
• TIR8MGC110992
• Toll/interleukin-1 receptor 8

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL1RAPL1), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Five of the IL-1 R family members (1, 2, 4, 5, and 6) are clustered and localized to chromosome 2. SIGIRR (single Ig domain containing IL-1 receptor-related molecule) is a subtype of the IL-1 R family that differs from the other nine members by having only one Ig domain in its extracellular region. The sequence of mouse SIGIRR predicts a 409 amino acid (aa) residue transmembrane glycoprotein that lacks signal peptide and contains a 117 aa single Ig extracellular domain, a transmembrane region and a 268 aa cytoplasmic tail with a TIR domain. The cytoplasmic tail of SIGIRR contains a C-terminal extention beyond the TIR domain which is also found in IL1RAPL1, IL-1 R9, and Toll-like receptor family members but absent in other IL-1 receptor family members. SIGIRR is widely expressed and is present in all cells and tissues examined. Mouse and human SIGIRR share 82% amino acid sequence identity. The ligand and signaling mechanism for SIGIRR has not been identified.

1. Thomassen, E. et al. (1999) Cytokine 11:389.

Publications for SIGIRR (992-SG)(1)

Publication using 992-SG

• Jiang X, McClellan SA, Barrett RP, Zhang Y, Hazlett LD Vasoactive intestinal peptide downregulates proinflammatory TLRs while upregulating anti-inflammatory TLRs in the infected cornea. J. Immunol., 2012-06-01;189(1):269-78. 2012-06-01 [PMID: 22661083] (Western Blot)

Bioinformatics

• Gene Symbol: Sigirr
• Uniprot:
  • Q9JLZ8()