Recombinant Mouse KGF/FGF-7 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse KGF/FGF-7 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 10-50 ng/mL.
Source
E. coli-derived mouse KGF/FGF-7 protein
Cys32-Thr194
Accession #
N-terminal Sequence
Cys32
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19 kDa, reducing conditions
Publications
Read Publications using
5028-KG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse KGF/FGF-7 Protein

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1 - 4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature mouse KGF, which is active across species, shares 96% aa sequence identity with human, rat, bovine, equine, and ovine forms.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

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Publications for KGF/FGF-7 (5028-KG)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(14)
ELISA (Standard)
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(14)
Rat
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 5028-KG Applications Species
DC Liberti, MM Kremp, WA Liberti, IJ Penkala, S Li, S Zhou, EE Morrisey Alveolar epithelial cell fate is maintained in a spatially restricted manner to promote lung regeneration after acute injury Cell Reports, 2021;35(6):109092. 2021 [PMID: 33979629] (Bioassay, Mouse) Bioassay Mouse
YH Kim, KJ Kim, DZ D'Argenio, ED Crandall Characteristics of Passive Solute Transport across Primary Rat Alveolar Epithelial Cell Monolayers Membranes, 2021;11(5):. 2021 [PMID: 33946241] (Bioassay, Rat) Bioassay Rat
K Obraztsova, MC Basil, R Rue, A Sivakumar, SM Lin, AR Mukhitov, AI Gritsiuta, JF Evans, M Kopp, J Katzen, A Robichaud, EN Atochina-V, S Li, J Carl, A Babu, MP Morley, E Cantu, MF Beers, DB Frank, EE Morrisey, VP Krymskaya mTORC1 activation in lung mesenchyme drives sex- and age-dependent pulmonary structure and function decline Nat Commun, 2020;11(1):5640. 2020 [PMID: 33159078] (Bioassay, Mouse) Bioassay Mouse
H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33128895] (Bioassay, Mouse) Bioassay Mouse
AJ Paris, KE Hayer, JH Oved, DC Avgousti, SA Toulmin, JA Zepp, WJ Zacharias, JB Katzen, MC Basil, MM Kremp, AR Slamowitz, S Jayachandr, A Sivakumar, N Dai, P Wang, DB Frank, LC Eisenlohr, E Cantu, MF Beers, MD Weitzman, EE Morrisey, GS Worthen STAT3-BDNF-TrkB signalling promotes alveolar epithelial regeneration after lung injury Nat Cell Biol, 2020;22(10):1197-1210. 2020 [PMID: 32989251] (Bioassay, Human) Bioassay Human
T Momozane, E Fukui, S Funaki, M Fujii, Y Kinehara, E Ito, S Miyagawa, Y Ohno, Y Sawa, M Okumura, Y Shintani Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor Stem Cells Int, 2019;2019(0):1271682. 2019 [PMID: 31949433] (Bioassay, Mouse) Bioassay Mouse
A Lepletier, ML Hun, MV Hammett, K Wong, H Naeem, M Hedger, K Loveland, AP Chidgey Interplay between Follistatin, Activin A, and BMP4 Signaling Regulates Postnatal Thymic Epithelial Progenitor Cell Differentiation during Aging Cell Rep, 2019;27(13):3887-3901.e4. 2019 [PMID: 31242421] (Bioassay, Mouse) Bioassay Mouse
S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 2019;9(1):8470. 2019 [PMID: 31186471] (Bioassay, Mouse) Bioassay Mouse
XY Liu, X Li, MR Bai, X Chen, CL Wang, J Xie, L Ye FGF-7 Dictates Osteocyte Cell Processes Through Beta-Catenin Transduction Sci Rep, 2018;8(1):14792. 2018 [PMID: 30287900] (Bioassay, Mouse) Bioassay Mouse
JD Glover, KL Wells, F Matthäus, KJ Painter, W Ho, J Riddell, JA Johansson, MJ Ford, CAB Jahoda, V Klika, RL Mort, DJ Headon Hierarchical patterning modes orchestrate hair follicle morphogenesis PLoS Biol., 2017;15(7):e2002117. 2017 [PMID: 28700594] (Bioassay, Mouse) Bioassay Mouse
Show All 16 Publications.

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