Recombinant Mouse KGF/FGF-7 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse KGF/FGF-7 Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED₅₀ for this effect is 10-50 ng/mL.
• Source: E. coli-derived mouse KGF/FGF-7 protein
  Cys32-Thr194
• Accession #: NP_032034
• N-terminal Sequence: Cys32
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse KGF/FGF-7 Protein

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1 - 4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature mouse KGF, which is active across species, shares 96% aa sequence identity with human, rat, bovine, equine, and ovine forms.

1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
8. Qiao, J. et al. (1999) Development 126:547.
9. Guo, L. et al. (1996) Genes Dev. 10:165.
10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
12. Belleudi, F. et al. (2007) Traffic 8:1854.

Publications for KGF/FGF-7 (5028-KG)(23)

Publications using 5028-KG

• Patel, VN;Aure, MH;Choi, SH;Ball, JR;Lane, ED;Wang, Z;Xu, Y;Zheng, C;Liu, X;Martin, D;Pailin, JY;Prochazkova, M;Kulkarni, AB;van Kuppevelt, TH;Ambudkar, IS;Liu, J;Hoffman, MP; Specific 3-O-sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation Nature communications 2024-08-31 [PMID: 39217171] (Bioassay, Mouse)
• Aure, MH;Symonds, JM;Villapudua, CU;Dodge, JT;Werner, S;Knosp, WM;Hoffman, MP; FGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation Nature communications 2023-10-14 [PMID: 37838739] (Bioassay, Mouse)
• DG Brownfield, AD de Arce, E Ghelfi, A Gillich, TJ Desai, MA Krasnow Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling Nature Communications, 2022-11-21;13(1):7137. 2022-11-21 [PMID: 36414616] (Bioassay, Mouse)
• N Hosaka, S Kanda, T Shimono, T Nishiyama Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells Journal of Cellular and Molecular Medicine, 2021-10-23;0(0):. 2021-10-23 [PMID: 34687276] (Cell Culture, Mouse)
• L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021-10-07;22(19):. 2021-10-07 [PMID: 34639189] (Western Blot, Mouse)
• Shaohe Wang, Kazue Matsumoto, Samantha R. Lish, Alexander X. Cartagena-Rivera, Kenneth M. Yamada Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion Cell 2021-07-01 [PMID: 34133940] (Bioassay, Mouse)
• DC Liberti, MM Kremp, WA Liberti, IJ Penkala, S Li, S Zhou, EE Morrisey Alveolar epithelial cell fate is maintained in a spatially restricted manner to promote lung regeneration after acute injury Cell Reports, 2021-05-11;35(6):109092. 2021-05-11 [PMID: 33979629] (Bioassay, Mouse)
• YH Kim, KJ Kim, DZ D'Argenio, ED Crandall Characteristics of Passive Solute Transport across Primary Rat Alveolar Epithelial Cell Monolayers Membranes, 2021-04-30;11(5):. 2021-04-30 [PMID: 33946241] (Bioassay, Rat)
• K Obraztsova, MC Basil, R Rue, A Sivakumar, SM Lin, AR Mukhitov, AI Gritsiuta, JF Evans, M Kopp, J Katzen, A Robichaud, EN Atochina-V, S Li, J Carl, A Babu, MP Morley, E Cantu, MF Beers, DB Frank, EE Morrisey, VP Krymskaya mTORC1 activation in lung mesenchyme drives sex- and age-dependent pulmonary structure and function decline Nat Commun, 2020-11-06;11(1):5640. 2020-11-06 [PMID: 33159078] (Bioassay, Mouse)
• H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction Cell Stem Cell, 2020-10-21;0(0):. 2020-10-21 [PMID: 33128895] (Bioassay, Mouse)
• AJ Paris, KE Hayer, JH Oved, DC Avgousti, SA Toulmin, JA Zepp, WJ Zacharias, JB Katzen, MC Basil, MM Kremp, AR Slamowitz, S Jayachandr, A Sivakumar, N Dai, P Wang, DB Frank, LC Eisenlohr, E Cantu, MF Beers, MD Weitzman, EE Morrisey, GS Worthen STAT3-BDNF-TrkB signalling promotes alveolar epithelial regeneration after lung injury Nat Cell Biol, 2020-09-28;22(10):1197-1210. 2020-09-28 [PMID: 32989251] (Bioassay, Human)
• T Momozane, E Fukui, S Funaki, M Fujii, Y Kinehara, E Ito, S Miyagawa, Y Ohno, Y Sawa, M Okumura, Y Shintani Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor Stem Cells Int, 2019-12-27;2019(0):1271682. 2019-12-27 [PMID: 31949433] (Bioassay, Mouse)
• A Lepletier, ML Hun, MV Hammett, K Wong, H Naeem, M Hedger, K Loveland, AP Chidgey Interplay between Follistatin, Activin A, and BMP4 Signaling Regulates Postnatal Thymic Epithelial Progenitor Cell Differentiation during Aging Cell Rep, 2019-06-25;27(13):3887-3901.e4. 2019-06-25 [PMID: 31242421] (Bioassay, Mouse)
• S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 2019-06-11;9(1):8470. 2019-06-11 [PMID: 31186471] (Bioassay, Mouse)
• XY Liu, X Li, MR Bai, X Chen, CL Wang, J Xie, L Ye FGF-7 Dictates Osteocyte Cell Processes Through Beta-Catenin Transduction Sci Rep, 2018-10-04;8(1):14792. 2018-10-04 [PMID: 30287900] (Bioassay, Mouse)
• Scott A. Rankin, Kyle W. McCracken, David M. Luedeke, Lu Han, James M. Wells, John M. Shannon et al. Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis Developmental Biology 2018-02-01 [PMID: 29217200] (Bioassay, Mouse, Transgenic Mouse)
• JD Glover, KL Wells, F Matthäus, KJ Painter, W Ho, J Riddell, JA Johansson, MJ Ford, CAB Jahoda, V Klika, RL Mort, DJ Headon Hierarchical patterning modes orchestrate hair follicle morphogenesis PLoS Biol., 2017-07-11;15(7):e2002117. 2017-07-11 [PMID: 28700594] (Bioassay, Mouse)
• H Zhang, J Luo, JF Alcorn, K Chen, S Fan, J Pilewski, A Liu, W Chen, JK Kolls, J Wang AIM2 Inflammasome Is Critical for Influenza-Induced Lung Injury and Mortality J. Immunol., 2017-04-19;0(0):. 2017-04-19 [PMID: 28424239] (Bioassay, Mouse)
• Z Zhang, K Newton, SK Kummerfeld, J Webster, DS Kirkpatric, L Phu, J Eastham-An, J Liu, WP Lee, J Wu, H Li, MR Junttila, VM Dixit Transcription factor Etv5 is essential for the maintenance of alveolar type II cells Proc. Natl. Acad. Sci. U.S.A, 2017-03-28;0(0):. 2017-03-28 [PMID: 28351980] (Bioassay, Mouse)
• Ray S, Fanti J, Macedo D, Larsen M LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell, 2014-06-25;25(16):2393-407. 2014-06-25 [PMID: 24966172] (Bioassay, Mouse)
• Tuckett , Andrea Z, Thornton , Raymond, Shono , Yusuke, Smith , Odette M, Levy , Emily R, Kreines , Fabiana, van den Brink , Marcel R, Zakrzewski , Johannes Image-guided intrathymic injection of multipotent stem cells supports lifelong T-cell immunity and facilitates targeted immunotherapy. Blood, 2014-03-20;123(18):2797-805. 2014-03-20 [PMID: 24652996] (In Vivo, Mouse)
• Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop. J Invest Dermatol, 2013-11-25;134(5):1295-304. 2013-11-25 [PMID: 24352037] (ELISA (Standard), Mouse)
• Sneddon , Julie B, Borowiak , Malgorza, Melton , Douglas Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012-10-07;491(7426):765-8. 2012-10-07 [PMID: 23041930] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • NP_032034()