Recombinant Mouse KGF/FGF-7 Protein, CF

Images

 
There are currently no images for KGF/FGF-7 (5028-KG/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse KGF/FGF-7 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 10-50 ng/mL.
Source
E. coli-derived mouse KGF/FGF-7 protein
Cys32-Thr194
Accession #
N-terminal Sequence
Cys32
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20 kDa, reducing conditions
Publications
Read Publications using
5028-KG/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse KGF/FGF-7 Protein, CF

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1 - 4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature mouse KGF, which is active across species, shares 96% aa sequence identity with human, rat, bovine, equine, and ovine forms.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

Customers Who Viewed This Item Also Viewed...

NBP2-00932
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB110-68800
Species: Hu, Mu
Applications: WB, ICC/IF
NB600-1287
Species: Hu, Mu, Rt
Applications: WB, Flow, IP, CyTOF-ready
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)

Publications for KGF/FGF-7 (5028-KG/CF)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(5)
ELISA Standard
(1)
In Vivo
(1)
All Applications
Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 5028-KG/CF Applications Species
JD Glover, KL Wells, F Matthäus, KJ Painter, W Ho, J Riddell, JA Johansson, MJ Ford, CAB Jahoda, V Klika, RL Mort, DJ Headon Hierarchical patterning modes orchestrate hair follicle morphogenesis PLoS Biol., 2017;15(7):e2002117. 2017 [PMID: 28700594] (Bioassay, Mouse) Bioassay Mouse
H Zhang, J Luo, JF Alcorn, K Chen, S Fan, J Pilewski, A Liu, W Chen, JK Kolls, J Wang AIM2 Inflammasome Is Critical for Influenza-Induced Lung Injury and Mortality J. Immunol., 2017;0(0):. 2017 [PMID: 28424239] (Bioassay, Mouse) Bioassay Mouse
Z Zhang, K Newton, SK Kummerfeld, J Webster, DS Kirkpatric, L Phu, J Eastham-An, J Liu, WP Lee, J Wu, H Li, MR Junttila, VM Dixit Transcription factor Etv5 is essential for the maintenance of alveolar type II cells Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28351980] (Bioassay, Mouse) Bioassay Mouse
Ray S, Fanti J, Macedo D, Larsen M LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell, 2014;25(16):2393-407. 2014 [PMID: 24966172] (Bioassay, Mouse) Bioassay Mouse
Tuckett, Andrea Z, Thornton, Raymond, Shono, Yusuke, Smith, Odette M, Levy, Emily R, Kreines, Fabiana, van den Brink, Marcel R, Zakrzewski, Johannes Image-guided intrathymic injection of multipotent stem cells supports lifelong T-cell immunity and facilitates targeted immunotherapy. Blood, 2014;123(18):2797-805. 2014 [PMID: 24652996] (In Vivo, Mouse) In Vivo Mouse
Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop. J Invest Dermatol, 2014;134(5):1295-304. 2014 [PMID: 24352037] (ELISA Standard, Mouse) ELISA Standard Mouse
Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012;491(7426):765-8. 2012 [PMID: 23041930] (Bioassay, Mouse) Bioassay Mouse

Reviews for KGF/FGF-7 (5028-KG/CF) (0)

There are no reviews for KGF/FGF-7 (5028-KG/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for KGF/FGF-7 (5028-KG/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse KGF/FGF-7 Protein, CF and receive a gift card or discount.

Bioinformatics

Entrez
Uniprot