Recombinant Mouse JAM-C Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse JAM-C Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes on immobilized JAM-2 (VE-JAM)/Fc Chimera. The ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 1 x 10⁵ cells/well. Each well was coated with 100 µL of a 0.2 µg/mL solution of rhJAM-2 (VE-JAM)/Fc Chimera.
• Source: Mouse myeloma cell line, NS0-derived mouse JAM-C protein
  

  Mouse JAM-C (Val32 - Asn241) Accession # Q9D8B7	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9D8B7
• N-terminal Sequence: Val32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Jam3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 64 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse JAM-C Fc Chimera Protein, CF

• CD323
• JAM-2
• JAM3
• JAMC
• JAM-C
• JAM-CFLJ14529
• junctional adhesion molecule 3JAM-3
• junctional adhesion molecule C

Background

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
2. Aurrand-Lions, M. et al. (2001) Blood 98:3699.
3. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
4. Johnson-Leger, C. et al. (2002) Blood 100:25793.

Publications for JAM-C (1213-J3)(6)

Publications using 1213-J3

• Pareja, J;Aydin, S;Zbinden, M;Bouillet, E;Zollinger, N;Theivendram, V;Fahmi, A;Pleska?, P;Barcos, S;Paas, F;Kloster, F;Blázquez, AM;Fonta, N;Merkler, D;Deutsch, U;Engelhardt, B; Lack of junctional adhesion molecule (JAM)-B traps CD8 T cells in CNS border zones and ameliorates autoimmune neuroinflammation Acta neuropathologica communications 2025-05-26 [PMID: 40420242] (Bioassay, Mouse)
• M Czabanka, LL Petrilli, S Elvers-Hor, K Bieback, B Albert Imh, P Vajkoczy, M Vinci Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis Int J Mol Sci, 2020-02-11;21(4):. 2020-02-11 [PMID: 32054130] (Cell Culture, Mouse)
• V Radulovic, M van der Ga, S Koide, V Sigurdsson, S Lang, S Kaneko, K Miharada Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis Cell Rep, 2019-06-04;27(10):2826-2836.e5. 2019-06-04 [PMID: 31167130] (Bioassay, Mouse)
• E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis Cell Adh Migr, 2016-04-25;10(4):419-33. 2016-04-25 [PMID: 27111582] (Flow Cytometry, Mouse)
• Su RW, Jia B, Ni H Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF. PLoS ONE, 2012-04-12;7(4):e34325. 2012-04-12 [PMID: 22511936] (Bioassay, Mouse)
• Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow. Blood, 2011-08-25;118(17):4609-19. 2011-08-25 [PMID: 21868569] (Adhesion Assay, Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Jam3
• Uniprot:
  • Q9D8B7()