JAM-C Antibody [Unconjugated]

Images

 
JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
JAM‑C was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse JAM‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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JAM-C Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM-C
Val32-Asn241
Accession # Q9D8B7
Specificity
Detects mouse JAM-C in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human JAM‑C and less than 2% cross-reactivity with recombinant mouse (rm) JAM-A and rmJAM-B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JAM3
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF1213 in the following applications:

Publications
Read Publications using
AF1213 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-C Antibody [Unconjugated]

  • CD323
  • JAM-2
  • JAM3
  • JAMC
  • JAM-C
  • JAM-CFLJ14529
  • junctional adhesion molecule 3JAM-3
  • junctional adhesion molecule C

Background

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurrand-Lions, M. et al. (2001) Blood 98:3699.
  3. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
  4. Johnson-Leger, C. et al. (2002) Blood 100:25793.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JAM-C Antibody (AF1213)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC-Fr, Neutralization, Western Blot.


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Flow Cytometry
(1)
ICC
(1)
IHC-Fr
(1)
Neutralization
(1)
Western Blot
(1)
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Human
(1)
Mouse
(4)
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Showing Publications 1 - 5 of 5.
Publications using AF1213 Applications Species
E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis Cell Adh Migr, 2016;10(4):419-33. 2016 [PMID: 27111582] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Wyss L, Schafer J, Liebner S, Mittelbronn M, Deutsch U, Enzmann G, Adams R, Aurrand-Lions M, Plate K, Imhof B, Engelhardt B Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus. PLoS ONE, 2012;7(9):e45619. 2012 [PMID: 23029139] (IHC-Fr, Mouse) IHC-Fr Mouse
Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci., 2009;50(3):1454-63. 2009 [PMID: 19060272] (ICC, Human) ICC Human
Fuse C, Ishida Y, Hikita T, Asai T, Oku N Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma. J. Biol. Chem., 2007;282(11):8276-83. 2007 [PMID: 17227766] (Western Blot, Mouse) Western Blot Mouse
Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation. J. Invest. Dermatol., 2005;125(5):969-76. 2005 [PMID: 16297198] (Neutralization, Mouse) Neutralization Mouse

Reviews for JAM-C Antibody (AF1213) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1213:
Filter by Applications
WB
(1)
IHC-P
(1)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Western Blot JAM-C AF1213
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reviewed by:
Jim Hsiao
WB Mouse 04/16/2018
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Summary

ApplicationWestern Blot
Sample TestedLung tissue
SpeciesMouse
LotJAJ0213071

Comments

CommentsPrimary antibody: Mouse JAM-C Antibody @ 1:500 in 5% milk/PBST
Secondary antibody: Donkey anti-Goat HRP @ 1:2500;
 
reviewed by:
Anonymous
IHC-P Mouse 01/05/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20531453
SpeciesMouse

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Secondary Antibodies

 

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Biotin BAF1213

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Jim Hsiao
04/16/2018
Application: WB
Species: Mouse

 
Anonymous
01/05/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol JAM3
Uniprot