JAM-C Antibody [Unconjugated]

JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

JAM‑C was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse JAM‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

JAM-C Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse JAM-C
  Val32-Asn241
  Accession # Q9D8B7
• Specificity: Detects mouse JAM-C in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human JAM‑C and less than 2% cross-reactivity with recombinant mouse (rm) JAM-A and rmJAM-B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: JAM3
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-C Antibody [Unconjugated]

• CD323
• JAM-2
• JAM3
• JAMC
• JAM-C
• JAM-CFLJ14529
• junctional adhesion molecule 3JAM-3
• junctional adhesion molecule C

Background

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
2. Aurrand-Lions, M. et al. (2001) Blood 98:3699.
3. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
4. Johnson-Leger, C. et al. (2002) Blood 100:25793.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAM-C Antibody (AF1213)(7)

Publications using AF1213

• J Li, X Tan, Q Sun, X Li, R Chen, L Luo Deficiency of Jamc Leads to Congenital Nuclear Cataract and Activates the Unfolded Protein Response in Mouse Lenses Investigative Ophthalmology & Visual Science, 2022;63(10):1. 2022 [PMID: 36048019] (Mouse)
• N McMillan, GW Kirschen, S Desai, E Xia, SE Tsirka, A Aguirre ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling Neural regeneration research, 2022;17(11):2472-2483. 2022 [PMID: 35535899] (Immunoprecipitation, Mouse)
• E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis Cell Adh Migr, 2016;10(4):419-33. 2016 [PMID: 27111582] (Flow Cytometry, Mouse)
• Wyss L, Schafer J, Liebner S, Mittelbronn M, Deutsch U, Enzmann G, Adams R, Aurrand-Lions M, Plate K, Imhof B, Engelhardt B Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus. PLoS ONE, 2012;7(9):e45619. 2012 [PMID: 23029139] (IHC-Fr, Mouse)
• Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium. Invest. Ophthalmol. Vis. Sci., 2009;50(3):1454-63. 2009 [PMID: 19060272] (ICC, Human)
• Fuse C, Ishida Y, Hikita T, Asai T, Oku N Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma. J. Biol. Chem., 2007;282(11):8276-83. 2007 [PMID: 17227766] (Western Blot, Mouse)
• Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation. J. Invest. Dermatol., 2005;125(5):969-76. 2005 [PMID: 16297198] (Neutralization, Mouse)

Reviews for JAM-C Antibody (AF1213) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 04/16/2018

Summary

• Application: Western Blot
• Sample Tested: Lung tissue
• Species: Mouse
• Lot: JAJ0213071

Comments

• Comments: Primary antibody: Mouse JAM-C Antibody @ 1:500 in 5% milk/PBST
  Secondary antibody: Donkey anti-Goat HRP @ 1:2500;

reviewed by: Anonymous - IHC-P Mouse 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 20531453
• Species: Mouse

Bioinformatics

• Gene Symbol: JAM3
• Uniprot:
  • Q9D8B7()