Recombinant Mouse IL-7 Protein

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1 μg/lane of Recombinant Mouse IL-7 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Mouse IL-7 (Catalog # 407‑ML) stimulates cell proliferation of PHA-activated human peripheral blood lymphocytes. The ED50 for this effect is 0.15-0.3 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-7 Protein Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.15-0.3 ng/mL.
Source
E. coli-derived mouse IL-7 protein
Glu26-Ile154, with an N-terminal Met
Accession #
N-terminal Sequence
Glu26
Protein/Peptide Type
Recombinant Proteins
Gene
Il7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
407-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-7 Protein

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). Mouse IL-7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide (4). Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

  1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
  2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
  4. Namen, A.E. et al. (1988) Nature 333:571.
  5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
  6. Saini, M. et al. (2009) Blood 113:5793.
  7. Park, J.H. et al. (2004) Immunity 21:289.
  8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
  11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
  12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
  15. Nodland, S.E. et al. (2011) Blood 118:2116.

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Publications for IL-7 (407-ML)(75)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Stimulation.


Filter By Application
Bioassay
(60)
Cell Culture
(6)
In Vivo
(5)
Stimulation
(1)
All Applications
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Human
(1)
Mouse
(72)
All Species
Showing Publications 1 - 10 of 75. Show All 75 Publications.
Publications using 407-ML Applications Species
S Steinmann, M Schoedsack, F Heinrich, PC Breda, A Ochel, G Tiegs, K Neumann Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells Sci Rep, 2020;10(1):1071. 2020 [PMID: 31974518] (Cell Culture, Mouse) Cell Culture Mouse
PT Mantani, P Dunér, I Ljungcrant, J Nilsson, H Björkbacka, GN Fredrikson ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions BMC Immunol., 2019;20(1):47. 2019 [PMID: 31823769] (Bioassay, Mouse) Bioassay Mouse
ELY Chen, PK Thompson, JC Zúñiga-Pfl RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors Nat. Immunol., 2019;0(0):. 2019 [PMID: 31636466] (Cell Culture, Mouse) Cell Culture Mouse
N Ron-Harel, JM Ghergurovi, G Notarangel, MW LaFleur, Y Tsubosaka, AH Sharpe, JD Rabinowitz, MC Haigis T Cell Activation Depends on Extracellular Alanine Cell Rep, 2019;28(12):3011-3021.e4. 2019 [PMID: 31533027] (Bioassay, Mouse) Bioassay Mouse
M Mandal, MK Okoreeh, DE Kennedy, M Maienschei, J Ai, KC McLean, N Kaverina, M Veselits, I Aifantis, F Gounari, MR Clark CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis Nat. Immunol., 2019;0(0):. 2019 [PMID: 31477919] (Bioassay, Mouse) Bioassay Mouse
B Maurer, H Nivarthi, B Wingelhofe, HTT Pham, M Schlederer, T Suske, R Grausenbur, AI Schiefer, M Prchal-Mur, D Chen, S Winkler, O Merkel, C Kornauth, M Hofbauer, B Hochgatter, G Hoermann, A Hoelbl-Kov, J Prochazkov, C Lobello, AA Cumaraswam, J Latzka, M Kitzwögere, A Chott, A Janikova, Š Pospíšilov, JI Loizou, S Kubicek, P Valent, T Kolbe, F Grebien, L Kenner, PT Gunning, R Kralovics, M Herling, M Müller, T Rülicke, V Sexl, R Moriggl High activation of STAT5A drives peripheral T-cell lymphoma and leukemia Haematologica, 2019;0(0):. 2019 [PMID: 31123029] (Bioassay, Mouse) Bioassay Mouse
DM Previte, CP Martins, EC O'Connor, ML Marre, GM Coudriet, NW Beck, AV Menk, RH Wright, HM Tse, GM Delgoffe, JD Piganelli Lymphocyte Activation Gene-3 Maintains Mitochondrial and Metabolic Quiescence in Naive CD4+ T Cells Cell Rep, 2019;27(1):129-141.e4. 2019 [PMID: 30943396] (Bioassay, Mouse) Bioassay Mouse
S Goossens, J Wang, C Tremblay, J De Medts, S T'Sas, T Nguyen, J Saw, K Haigh, DJ Curtis, P Van Vlierb, G Berx, T Taghon, JJ Haigh ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms Haematologica, 2019;0(0):. 2019 [PMID: 30679322] (Bioassay, Mouse) Bioassay Mouse
C Gérard, C Hubeau, O Carnet, M Bellefroid, NE Sounni, S Blacher, G Bendavid, M Moser, R Fässler, A Noel, D Cataldo, N Rocks Microenvironment-derived ADAM28 prevents cancer dissemination Oncotarget, 2018;9(98):37185-37199. 2018 [PMID: 30647853] (Bioassay, Mouse) Bioassay Mouse
C Konopacki, Y Pritykin, Y Rubtsov, CS Leslie, AY Rudensky Transcription factor Foxp1 regulates Foxp3 chromatin binding and coordinates regulatory T cell function Nat. Immunol., 2019;20(2):232-242. 2019 [PMID: 30643266] (Bioassay, Mouse) Bioassay Mouse
Show All 75 Publications.

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Bioinformatics

Gene Symbol Il7
Uniprot