Recombinant Mouse IL-6 Protein


1 μg/lane of Recombinant Mouse IL-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.
Recombinant Mouse IL-6 (Catalog # 406‑ML) stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 0.02‑0.06 ng/mL.

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Recombinant Mouse IL-6 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.06 ng/mL.
E. coli-derived mouse IL-6 protein
Phe25-Thr211, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
406-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and EDTA with Trehalose and with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-6 Protein

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta-2
  • IL6
  • IL-6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A


Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1 - 5). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 39% and 85% aa sequence identity with human and rat IL-6, respectively (6 - 8). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (9). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (10). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 11). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1 - 5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 12).
  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  4. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  5. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
  6. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  7. Simpson, R.J. et al. (1988) Eur. J. Biochem. 176:187.
  8. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193.
  9. Murakami, M. et al. (1993) Science 260:1808.
  10. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  11. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  12. Cerutti, A. et al. (1998) J. Immunol. 160:2145.

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Publications for IL-6 (406-ML)(210)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, ELISA Standard, In Vivo, InVivo, WB Ctrl, cell culture.

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Showing Publications 1 - 10 of 210. Show All 210 Publications.
Publications using 406-ML Applications Species
B Zwarycz, AD Gracz, KR Rivera, IA Williamson, LA Samsa, J Starmer, MA Daniele, L Salter-Cid, Q Zhao, ST Magness IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model Cell Mol Gastroenterol Hepatol, 2019;7(1):1-17. 2019 [PMID: 30364840] (Bioassay, Mouse) Bioassay Mouse
A Badamchi-Z, KD Moynihan, RA Larocca, M Aid, NM Provine, MJ Iampietro, E Kinnear, P Penaloza-M, P Abbink, E Blass, JS Tregoning, DJ Irvine, DH Barouch Combined HDAC and BET Inhibition Enhances Melanoma Vaccine Immunogenicity and Efficacy J. Immunol., 2018;0(0):. 2018 [PMID: 30249811] (In Vivo, Mouse) In Vivo Mouse
HC Chuang, CY Tsai, CH Hsueh, TH Tan GLK-IKK? signaling induces dimerization and translocation of the AhR-ROR?t complex in IL-17A induction and autoimmune disease Sci Adv, 2018;4(9):eaat5401. 2018 [PMID: 30214937] (Bioassay, Mouse) Bioassay Mouse
HA Ting, D de Almeida, AJ Rasky, CA Malinczak, IP Maillard, MA Schaller, NW Lukacs Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection Mucosal Immunol, 2018;0(0):. 2018 [PMID: 30038214] (Bioassay, Mouse) Bioassay Mouse
CC Hsieh, CL Lin, JT He, M Chiang, Y Wang, YC Tsai, CH Hung, PJ Chang Administration of cytokine-induced myeloid-derived suppressor cells ameliorates renal fibrosis in diabetic mice Stem Cell Res Ther, 2018;9(1):183. 2018 [PMID: 29973247] (Bioassay, Mouse) Bioassay Mouse
E Archer-Lah, C Lan, RT Jagoe Physiological culture conditions alter myotube morphology and responses to atrophy treatments: implications for in�vitro research on muscle wasting Physiol Rep, 2018;6(12):e13726. 2018 [PMID: 29932505] (Bioassay, Mouse) Bioassay Mouse
Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis PLoS ONE, 2018;13(6):e0198740. 2018 [PMID: 29879214] (Bioassay, Mouse) Bioassay Mouse
YY Wang, S Ma, Q Chen, D Jiao, Y Yang In vivo selection with lentiviral expression of Bcl2T69A/S70A/S87Amutant in hematopoietic stem cell-transplanted mice Gene Ther., 2018;0(0):. 2018 [PMID: 29523881] (Bioassay, Mouse) Bioassay Mouse
R Yang, T Yu, D Liu, S Shi, Y Zhou Hydrogen sulfide promotes immunomodulation of gingiva-derived mesenchymal stem cells via the Fas/FasL coupling pathway Stem Cell Res Ther, 2018;9(1):62. 2018 [PMID: 29523215] (Bioassay, Mouse) Bioassay Mouse
KL Garner, VMS Betin, V Pinto, M Graham, E Abgueguen, M Barnes, DC Bedford, CA McArdle, RJM Coward Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress Sci Rep, 2018;8(1):3902. 2018 [PMID: 29500363] (Bioassay, Mouse) Bioassay Mouse
Show All 210 Publications.

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FAQs for IL-6 (406-ML). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
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Gene Symbol Il6