Recombinant Mouse IL-6 Protein


1 μg/lane of Recombinant Mouse IL-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.
Recombinant Mouse IL-6 (Catalog # 406‑ML) stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 0.02‑0.06 ng/mL.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse IL-6 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.06 ng/mL.
E. coli-derived
Phe25-Thr211, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
406-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and EDTA with Trehalose and with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-6 Protein

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta-2
  • IL6
  • IL-6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A


Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1 - 5). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 39% and 85% aa sequence identity with human and rat IL-6, respectively (6 - 8). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (9). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (10). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 11). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1 - 5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 12).

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Publications for IL-6 (406-ML)(191)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, ELISA Standard, In Vivo, InVivo, WB Ctrl, cell culture.

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Showing Publications 1 - 10 of 191. Show All 191 Publications.
Publications using 406-ML Applications Species
QH Wang, SW Lv, YY Guo, JX Duan, SY Dong, QS Wang, FM Yu, H Su, HX Kuang Pharmacological Effect ofCaulophyllum robustumon Collagen-Induced Arthritis and Regulation of Nitric Oxide, NF-?B, and Proinflammatory Cytokines In Vivo and In Vitro Evid Based Complement Alternat Med, 2017;2017(0):8134321. 2017 [PMID: 29456573] (Bioassay, Mouse) Bioassay Mouse
SK Varanasi, U Jaggi, N Hay, BT Rouse Hexokinase II may be dispensable for CD4 T cell responses against a virus infection PLoS ONE, 2018;13(1):e0191533. 2018 [PMID: 29352298] (Bioassay, Mouse) Bioassay Mouse
JE Draper, P Sroczynska, MZH Fadlullah, R Patel, G Newton, W Breitwiese, V Kouskoff, G Lacaud A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects PLoS Genet., 2018;14(1):e1007127. 2018 [PMID: 29300724] (Bioassay, Mouse) Bioassay Mouse
X Sun, K Gupta, B Wu, D Zhang, B Yuan, X Zhang, HC Chiang, C Zhang, TJ Curiel, MP Bendeck, S Hursting, Y Hu, R Li Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulatingadipose stromal interleukin 6 production in mice J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29298894] (Bioassay, Mouse) Bioassay Mouse
P Barragán-I, TF Lou, VD Bhat, S Megat, MD Burton, TJ Price, ZT Campbell Inhibition of Poly(A)-binding protein with a synthetic RNA mimic reduces pain sensitization in mice Nat Commun, 2018;9(1):10. 2018 [PMID: 29295980] (In Vivo, Mouse) In Vivo Mouse
X Jiang, C Hu, K Ferchen, J Nie, X Cui, CH Chen, L Cheng, Z Zuo, W Seibel, C He, Y Tang, JR Skibbe, M Wunderlich, WC Reinhold, L Dong, C Shen, S Arnovitz, B Ulrich, J Lu, H Weng, R Su, H Huang, Y Wang, C Li, X Qin, J Mulloy, Y Zheng, J Diao, J Jin, C Li, PP Liu, C He, Y Chen, J Chen Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia Nat Commun, 2017;8(1):2099. 2017 [PMID: 29235481] (Bioassay, Mouse) Bioassay Mouse
S Ollila, E Domènech-M, K Laajanen, IP Wong, S Tripathi, N Pentinmikk, Y Gao, Y Yan, EH Niemelä, TC Wang, B Viollet, G Leone, P Katajisto, K Vaahtomeri, TP Mäkelä Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway J. Clin. Invest., 2017;0(0):. 2017 [PMID: 29202476] (Bioassay, Mouse) Bioassay Mouse
W Eichler, H Savkovi?-C, S Bürger, M Beck, M Schmidt, P Wiedemann, A Reichenbac, JD Unterlauft M�ller Cell-Derived PEDF Mediates Neuroprotection via STAT3 Activation Cell. Physiol. Biochem., 2017;44(4):1411-1424. 2017 [PMID: 29186716] (Bioassay, Mouse) Bioassay Mouse
A Hammer, A Schliep, S Jörg, A Haghikia, R Gold, M Kleinewiet, DN Müller, RA Linker Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation J Neuroinflammation, 2017;14(1):184. 2017 [PMID: 28899400] (Bioassay, Mouse) Bioassay Mouse
T Oike, Y Sato, T Kobayashi, K Miyamoto, S Nakamura, Y Kaneko, S Kobayashi, K Harato, H Saya, M Matsumoto, M Nakamura, Y Niki, T Miyamoto Stat3 as a potential therapeutic target for rheumatoid arthritis Sci Rep, 2017;7(1):10965. 2017 [PMID: 28887478] (Bioassay, Mouse) Bioassay Mouse
Show All 191 Publications.

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FAQs for IL-6 (406-ML). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
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Gene Symbol Il6