Recombinant Mouse IL-6 Protein


1 μg/lane of Recombinant Mouse IL-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.
Recombinant Mouse IL-6 (Catalog # 406‑ML) stimulates cell proliferation of the T1165.85.2.1 mouse plasmacytoma cell line. The ED50 for this effect is 0.02‑0.06 ng/mL.

Product Details

Reactivity MuSpecies Glossary
Applications BA

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Recombinant Mouse IL-6 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.06 ng/mL.
E. coli-derived
Phe25-Thr211, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
406-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and EDTA with Trehalose and with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-6 Protein

  • B cell stimulatory factor-2
  • B-cell differentiation factor
  • BSF-2
  • BSF2CTL differentiation factor
  • CDF
  • HGFHSFIFNB2Hybridoma growth factor
  • IFN-beta-2
  • IL6
  • IL-6
  • IL-6B-cell stimulatory factor 2
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A


Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1 - 5). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 39% and 85% aa sequence identity with human and rat IL-6, respectively (6 - 8). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (9). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (10). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 11). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1 - 5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 12).

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Publications for (183)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Bioassay, ELISA Standard, In Vivo, InVivo, WB Ctrl, cell culture.

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Showing Publications 1 - 10 of 183. Show All 183 Publications.
Publications using 406-ML Applications Species
A Hammer, A Schliep, S Jörg, A Haghikia, R Gold, M Kleinewiet, DN Müller, RA Linker Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation J Neuroinflammation, 2017;14(1):184. 2017 [PMID: 28899400] (Bioassay, Mouse) Bioassay Mouse
T Oike, Y Sato, T Kobayashi, K Miyamoto, S Nakamura, Y Kaneko, S Kobayashi, K Harato, H Saya, M Matsumoto, M Nakamura, Y Niki, T Miyamoto Stat3 as a potential therapeutic target for rheumatoid arthritis Sci Rep, 2017;7(1):10965. 2017 [PMID: 28887478] (Bioassay, Mouse) Bioassay Mouse
N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill,, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction JCI Insight, 2017;2(17):. 2017 [PMID: 28878126] (Bioassay, Mouse) Bioassay Mouse
SH Lee, JY Kwon, SY Kim, K Jung, ML Cho Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis Sci Rep, 2017;7(1):10133. 2017 [PMID: 28860618] (Bioassay, Mouse) Bioassay Mouse
A Muschaweck, F Petermann, T Korn IL-1? and IL-23 Promote Extrathymic Commitment of CD27(+)CD122(-) ?? T Cells to ??T17 Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28855314] (Bioassay, Mouse) Bioassay Mouse
MH Ross, AK Esser, GC Fox, AH Schmieder, X Yang, G Hu, D Pan, X Su, Y Xu, DV Novack, T Walsh, GA Colditz, GH Lukaszewic, E Cordell, JS Novack, JAJ Fitzpatric, DL Waning, KS Mohammad, TA Guise, GM Lanza, KN Weilbaeche Bone-induced expression of integrin ?3 enables targeted nanotherapy of breast cancer metastases Cancer Res., 2017;0(0):. 2017 [PMID: 28855208] (Bioassay, Mouse) Bioassay Mouse
KA Read, MD Powell, CE Baker, BK Sreekumar, VM Ringel-Sca, H Bachus, RE Martin, ID Cooley, IC Allen, A Ballestero, KJ Oestreich Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4(+) Th Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28848064] (Bioassay, Mouse) Bioassay Mouse
T Hosokawa, T Kimura, S Nada, T Okuno, D Ito, S Kang, S Nojima, K Yamashita, T Nakatani, Y Hayama, Y Kato, Y Kinehara, M Nishide, N Mikami, S Koyama, H Takamatsu, D Okuzaki, N Ohkura, S Sakaguchi, M Okada, A Kumanogoh Lamtor1 Is Critically Required for CD4(+) T Cell Proliferation and Regulatory T Cell Suppressive Function J. Immunol., 2017;0(0):. 2017 [PMID: 28768723] (Bioassay, Mouse) Bioassay Mouse
M Fernández, EM Monsalve, S López-Lópe, A Ruiz-Garcí, S Mellado, E Caminos, JJ García-Ram, J Laborda, P Tranque, MJM Díaz-Guerr Absence of Notch1 in murine myeloid cells attenuates the development of experimental autoimmune encephalomyelitis by affecting Th1 and Th17 priming Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28762472] (Bioassay, Mouse) Bioassay Mouse
TM Nordmann, E Dror, F Schulze, S Traub, E Berishvili, C Barbieux, M Böni-Schne, MY Donath The Role of Inflammation in ?-cell Dedifferentiation Sci Rep, 2017;7(1):6285. 2017 [PMID: 28740254] (Bioassay, Mouse) Bioassay Mouse
Show All 183 Publications.

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FAQs for . (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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TLR2 - I can recognize many foreign pathogens!
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Gene Symbol Il6