Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF

Recombinant Mouse IL-23 (Catalog # 11269-ML) has a molecular weight (MW) of 60.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.050-0.500 ng/mL.

2 μg/lane of Recombinant Mouse IL-23 (HEK293-expressed) Protein (Catalog # 11269-ML) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS.
• Details of Functionality: Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED₅₀ for this effect is 0.050-0.500 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse IL-23 protein
  

  Mouse IL-12p40 (Met22-Ser335) Accession # P43432.1	GSGSSRGGSGSGGSGGGGSKL	Mouse IL-23p19 (Leu20-Ala196) Accession # Q9EQ14.1
  N-terminus		C-terminus

• Accession #: P43432.1 (p40) & Q9EQ14.1 (p19)
• N-terminal Sequence: Met22
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-75 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100-500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-23 (HEK293-expressed) Protein, CF

• IL-23 p19/IL-12 p40
• IL23
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL-23p19/IL-12p40
• IL23P19P19
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-23 subunit alpha
• Interleukin-23 subunit p19
• JKA3 induced upon T-cell activation
• MGC79388
• SGRF
• SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human Tnbsp;cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5699.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Publications for IL-23A/IL-23 P19 (11269-ML)(2)

Publications using 11269-ML

• Beausoleil, S;Ariss, M;Huang, L;Ding, X;Sheth, S;Levy, T;Fisher, J;Loebelenz, J;Arlotta, K;Dixon, K;Polakiewicz, R;Kuchroo, V; InTraSeq: A Multimodal Assay that Uncovers New Single-Cell Biology and Regulatory Mechanisms Research square 2024-12-09 [PMID: 39711533] (Bioassay, Mouse)
• Parker, ME;Mehta, NU;Liao, TC;Tomaszewski, WH;Snyder, SA;Busch, J;Ciofani, M; Restriction of innate T??17 cell plasticity by an AP-1 regulatory axis bioRxiv : the preprint server for biology 2024-10-18 [PMID: 39463970] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • P43432.1 (p40) & Q9EQ14.1 (p19)()