Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

Images

 
When Recombinant Mouse IL‑18 R beta /IL‑1 R7 Fc Chimera(Catalog # 9459-AP) is coated at 2 μg/mL (100 μL /well), Recombinant Mouse IL‑18/IL‑1F4 (Catalog # 9139-IL) binds with an ED50 of 1-6 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑18 R beta /IL‑1 R7 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL‑18/IL‑F4 (Catalog # 9139‑IL) that produces 50% optimal binding response is 1-6 μg/mL
Source
Mouse myeloma cell line, NS0-derived mouse IL-18 R beta/IL-1 R7 protein
Mouse IL-18 R beta /IL-1 R7
(Glu15 & Phe20-Asp357)
Accession # XP_006495773
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu15, Phe20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
74-95 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

  • Accessory protein-like
  • AcPL
  • CD218 antigen-like family member B
  • CD218b antigen
  • CD218b
  • CDw218b
  • IL-1 R7
  • IL-18 R beta
  • IL-18 receptor accessory protein
  • IL-18 receptor beta
  • IL18R beta
  • IL-18RAcP
  • IL18RAP
  • IL18RB
  • IL-18Rb
  • IL-18Rbeta
  • IL-18R-beta
  • IL-1R accessory protein-like
  • IL1R7
  • IL-1R7
  • IL-1R-7
  • IL-1RAcPL
  • interleukin 18 receptor accessory protein
  • Interleukin-1 receptor 7
  • interleukin-18 receptor accessory protein
  • Interleukin-18 receptor accessory protein-like
  • Interleukin-18 receptor beta
  • MGC120589
  • MGC120590

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses (1). The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness (1, 2). Mouse IL-18 R beta cDNA encodes a 614 amino acid (aa) residue precursor type I membrane protein with a 19 aa signal peptide, a 337 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 237 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon (1).
  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Wu, C., et al.  (2003) J. Immunol. 273:5571.

Customers Who Viewed This Item Also Viewed...

MAB840
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ICC, Neut
NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
NBP2-00641
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
MAB155
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-88826
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-201
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
9459-AP
Species: Mu
Applications: Bioactivity

Publications for IL-18 R beta/IL-1 R7/ACPL (9459-AP) (0)

There are no publications for IL-18 R beta/IL-1 R7/ACPL (9459-AP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-18 R beta/IL-1 R7/ACPL (9459-AP) (0)

There are no reviews for IL-18 R beta/IL-1 R7/ACPL (9459-AP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for IL-18 R beta/IL-1 R7/ACPL (9459-AP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF and receive a gift card or discount.

Bioinformatics

Entrez
Uniprot