Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

When Recombinant Mouse IL‑18 R beta /IL‑1 R7 Fc Chimera(Catalog # 9459-AP) is coated at 2 μg/mL (100 μL /well), Recombinant Mouse IL‑18/IL‑1F4 (Catalog # 9139-IL) binds with an ED50 of 1-6 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑18 R beta /IL‑1 R7 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL‑18/IL‑F4 (Catalog # 9139‑IL) that produces 50% optimal binding response is 1-6 μg/mL
• Source: Mouse myeloma cell line, NS0-derived mouse IL-18 R beta/IL-1 R7 protein
  

  Mouse IL-18 R beta /IL-1 R7 (Glu15 & Phe20-Asp357) Accession # XP_006495773	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: XP_006495773
• N-terminal Sequence: Glu15, Phe20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 74-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

• Accessory protein-like
• AcPL
• CD218 antigen-like family member B
• CD218b antigen
• CD218b
• CDw218b
• IL-1 R7
• IL-18 R beta
• IL-18 receptor accessory protein
• IL-18 receptor beta
• IL18R beta
• IL-18RAcP
• IL18RAP
• IL18RB
• IL-18Rb
• IL-18Rbeta
• IL-18R-beta
• IL-1R accessory protein-like
• IL1R7
• IL-1R7
• IL-1R-7
• IL-1RAcPL
• interleukin 18 receptor accessory protein
• Interleukin-1 receptor 7
• interleukin-18 receptor accessory protein
• Interleukin-18 receptor accessory protein-like
• Interleukin-18 receptor beta
• MGC120589
• MGC120590

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses (1). The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness (1, 2) . Mouse IL-18 R beta cDNA encodes a 614 amino acid (aa) residue precursor type I membrane protein with a 19 aa signal peptide, a 337 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 237 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon (1).

1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
2. Wu, C., et al.  (2003) J. Immunol. 273:5571.

Bioinformatics

• Uniprot:
  • XP_006495773()