Recombinant Mouse IL-10 Protein

Images

 
1 μg/lane of Recombinant Mouse IL-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Mouse IL-10 (Catalog # 417‑ML) stimulates cell proliferation of the MC/9‑2 mouse mast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse IL-10 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.1‑0.6 ng/mL.
Source
E. coli-derived mouse IL-10 protein
Ser19-Ser178
Accession #
N-terminal Sequence
Ser19
Structure / Form
Noncovalently-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il10
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
417-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-10 Protein

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Moore, K. et al. (1990) Science 248:1230.
  9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  10. Syto, R. et al. (1998) Biochemistry 37:16943.
  11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

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Publications for IL-10 (417-ML)(43)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), In Vivo, Luminex (Standard).


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Bioassay
(24)
ELISA (Standard)
(8)
In Vivo
(9)
Luminex (Standard)
(1)
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Filter By Species
Human
(1)
Mouse
(35)
N/A
(6)
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Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 417-ML Applications Species
I Hayek, F Fischer, J Schulze-Lu, K Dettmer, K Sobotta, V Schatz, L Kohl, K Boden, R Lang, PJ Oefner, S Wirtz, J Jantsch, A Lührmann Limitation of TCA Cycle Intermediates Represents an Oxygen-Independent Nutritional Antibacterial Effector Mechanism of Macrophages Cell Rep, 2019;26(13):3502-3510.e6. 2019 [PMID: 30917307] (Bioassay, Mouse) Bioassay Mouse
K Paduch, A Debus, B Rai, U Schleicher, C Bogdan Resolution of Cutaneous Leishmaniasis and Persistence of Leishmania major in the Absence of Arginase 1 J. Immunol., 2019;0(0):. 2019 [PMID: 30665936] (Bioassay, Mouse) Bioassay Mouse
Maria J Forteza, Konstantinos A Polyzos, Roland Baumgartn, Bianca E Suur, Marion Mussbache, Daniel K Johansson, Andreas Hermansso, Göran K Hansson, Daniel F J Ketelhuth Activation of the Regulatory T-Cell/Indoleamine 2,3-Dioxygenase Axis Reduces Vascular Inflammation and Atherosclerosis in Hyperlipidemic Mice. Frontiers in Immunology, 2018;0(0):1664-3224. 2018 [PMID: 29867939] (Bioassay, Mouse) Bioassay Mouse
K Kanai, AM Park, A Watanabe, T Arikawa, T Yasui, H Yoshida, I Tsunoda, O Yoshie Murine ?-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin J. Immunol., 2018;0(0):. 2018 [PMID: 29500240] (In Vivo, Mouse) In Vivo Mouse
SMP Vadevoo, JE Kim, GR Gunassekar, HK Jung, L Chi, DE Kim, SH Lee, SH Im, B Lee Interleukin 4 receptor-targeted pro-apoptotic peptide blocks tumor growth and metastasis by enhancing anti-tumor immunity Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28878029] (Bioassay, Mouse) Bioassay Mouse
YY Sin, PR Price, LL Ballantyne, CD Funk Proof-of-Concept Gene Editing for the Murine Model of Inducible Arginase-1 Deficiency Sci Rep, 2017;7(1):2585. 2017 [PMID: 28566761] (Bioassay, Mouse) Bioassay Mouse
B Jee, P Sharma, K Katoch, B Joshi, SK Awasthi IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage Braz J Infect Dis, 2017;0(0):. 2017 [PMID: 28435012] (Bioassay, Mouse) Bioassay Mouse
CF Chang, J Wan, Q Li, SC Renfroe, NM Heller, J Wang Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage Neurobiol. Dis., 2017;103(0):54-69. 2017 [PMID: 28365213] (In Vivo, Mouse) In Vivo Mouse
W Dawicki, C Li, J Town, X Zhang, JR Gordon Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3. 2017 [PMID: 28277274] (ELISA (Standard), Mouse) ELISA (Standard) Mouse
S Gao, C Li, Y Zhu, Y Wang, A Sui, Y Zhong, B Xie, X Shen PEDF mediates pathological neovascularization by regulating macrophage recruitment and polarization in the mouse model of oxygen-induced retinopathy Sci Rep, 2017;7(0):42846. 2017 [PMID: 28211523] (Bioassay, Mouse) Bioassay Mouse
Show All 43 Publications.

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By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

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Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol Il10
Uniprot