Recombinant Mouse HGF Protein

Images

 
There are currently no images for HGF (2207-HG).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity, Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse HGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 10-30 ng/mL. Measured by its ability to bind Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 7065-ME) in a functional ELISA with an estimated KD <0.2 nM.
Source
Mouse myeloma cell line, NS0-derived mouse HGF protein
Gln33-Arg495 (alpha) & Val496-Leu728 (beta)
Accession #
N-terminal Sequence
No results obtained: Gln33 predicted (alpha) & Val496 (beta)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Hgf
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
53.4 kDa ( alpha -chain), 26 kDa ( beta -chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa and 33 kDa, reducing conditions
Publications
Read Publications using
2207-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91% - 95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF‑dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Customers Who Viewed This Item Also Viewed...

AF276
Species: Hu
Applications: WB, Flow, IHC, Block, CyTOF-ready, ICC, KO
NBP1-80841
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for HGF (2207-HG)(41)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Bioassay, Cell Culture, Ex Vivo, In Vivo.


Filter By Application
Bioassay
(28)
Cell Culture
(1)
Ex Vivo
(1)
In Vivo
(11)
All Applications
Filter By Species
Human
(1)
Mouse
(38)
Rat
(2)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using 2207-HG Applications Species
A Chiche, A Di-Cicco, L Sesma-Sanz, L Bresson, P de la Gran, MA Glukhova, MM Faraldo, MA Deugnier p53 controls the plasticity of mammary luminal progenitor cells downstream of Met signaling Breast Cancer Res., 2019;21(1):13. 2019 [PMID: 30683141] (Bioassay, Mouse) Bioassay Mouse
NF Bonan, D Kowalski, K Kudlac, K Flaherty, JC Gwilliam, LG Falkenberg, E Maradiaga, KL DeCicco-Sk Inhibition of HGF/MET signaling decreases overall tumor burden and blocks malignant conversion in Tpl2-related skin cancer Oncogenesis, 2019;8(1):1. 2019 [PMID: 30631034] (Bioassay, Mouse) Bioassay Mouse
C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA Sci Rep, 2018;8(1):12341. 2018 [PMID: 30120295] (Bioassay, Mouse) Bioassay Mouse
Y Jing, Q Sun, X Xiong, R Meng, S Tang, S Cao, Y Bi, D Zhu Hepatocyte Growth Factor alleviates hepatic insulin resistance and lipid accumulation in high-fat diet-fed mice J Diabetes Investig, 2018;0(0):. 2018 [PMID: 30070033] (In Vivo, Mouse) In Vivo Mouse
JA Zepp, J Zhao, C Liu, K Bulek, L Wu, X Chen, Y Hao, Z Wang, X Wang, W Ouyang, MF Kalady, J Carman, WP Yang, J Zhu, C Blackburn, YH Huang, TA Hamilton, B Su, X Li IL-17A-Induced PLET1 Expression Contributes to Tissue Repair and Colon Tumorigenesis J. Immunol., 2017;0(0):. 2017 [PMID: 29070673] (Bioassay, Mouse) Bioassay Mouse
E Paldy, M Simonetti, T Worzfeld, KK Bali, L Vicuña, S Offermanns, R Kuner Semaphorin 4C Plexin-B2 signaling in peripheral sensory neurons is pronociceptive in a model of inflammatory pain Nat Commun, 2017;8(1):176. 2017 [PMID: 28765520] (In Vivo, Mouse) In Vivo Mouse
SC Shih, TC Ho, SL Chen, YP Tsao Pigment epithelium-derived factor (PEDF) peptide promotes the expansion of hepatic stem/progenitor cells via ERK and STAT3-dependent signaling Am J Transl Res, 2017;9(3):1114-1126. 2017 [PMID: 28386338] (Bioassay, Mouse) Bioassay Mouse
Z Zhang, K Newton, SK Kummerfeld, J Webster, DS Kirkpatric, L Phu, J Eastham-An, J Liu, WP Lee, J Wu, H Li, MR Junttila, VM Dixit Transcription factor Etv5 is essential for the maintenance of alveolar type II cells Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28351980] (Bioassay, Mouse) Bioassay Mouse
K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke BMP-9 interferes with liver regeneration and promotes liver fibrosis Gut, 2017;0(0):. 2017 [PMID: 28336518] (Bioassay, Mouse) Bioassay Mouse
V Kochat, Z Equbal, P Baligar, V Kumar, M Srivastava, A Mukhopadhy JMJD3 aids in reprogramming of bone marrow progenitor cells to hepatic phenotype through epigenetic activation of hepatic transcription factors PLoS ONE, 2017;12(3):e0173977. 2017 [PMID: 28328977] (Bioassay, Mouse) Bioassay Mouse
Show All 41 Publications.

Reviews for HGF (2207-HG) (0)

There are no reviews for HGF (2207-HG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HGF (2207-HG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HGF Products

Bioinformatics Tool for HGF (2207-HG)

Discover related pathways, diseases and genes to HGF (2207-HG). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HGF (2207-HG)

Discover more about diseases related to HGF (2207-HG).
 

Pathways for HGF (2207-HG)

View related products by pathway.

PTMs for HGF (2207-HG)

Learn more about PTMs related to HGF (2207-HG).

Blogs on HGF.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse HGF Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Hgf
Uniprot