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Recombinant Mouse HGF Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity, Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse HGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 10-30 ng/mL. Measured by its ability to bind Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 7065-ME) in a functional ELISA with an estimated KD <0.2 nM.
Source
Mouse myeloma cell line, NS0-derived mouse HGF protein
Gln33-Arg495 (alpha) & Val496-Leu728 (beta)
Accession #
N-terminal Sequence
No results obtained: Gln33 predicted (alpha) & Val496 (beta)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Hgf
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
  • Bioactivity
Theoretical MW
53.4 kDa ( alpha -chain), 26 kDa ( beta -chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa and 33 kDa, reducing conditions
Publications
Read Publications using
2207-HG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91% - 95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF‑dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

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Publications for HGF (2207-HG)(59)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), Ex Vivo, In Vivo.


Filter By Application
Bioassay
(43)
Cell Culture
(5)
ELISA (Standard)
(1)
Ex Vivo
(1)
In Vivo
(12)
All Applications
Filter By Species
Human
(5)
Mouse
(51)
Rat
(4)
All Species
Showing Publications 1 - 10 of 59. Show All 59 Publications.
Publications using 2207-HG Applications Species
Rizzoti, K;Chakravarty, P;Sheridan, D;Lovell-Badge, R; SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on context Science advances 2023-10-06 [PMID: 37792947] (Bioassay, Mouse) Bioassay Mouse
Gerardo-Ram�rez, M;Giam, V;Becker, D;Groth, M;Hartmann, N;Morrison, H;May-Simera, HL;Radsak, MP;Marquardt, JU;Galle, PR;Herrlich, P;Straub, BK;Hartmann, M; Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2-Mutant Mice Cells 2023-04-26 [PMID: 37174657] (Bioassay, Mouse) Bioassay Mouse
M Carbonaro, K Wang, H Huang, D Frleta, A Patel, A Pennington, M Desclaux, S Moller-Tan, J Grindley, J Altarejos, J Zhong, G Polites, W Poueymirou, S Jaspers, C Kyratsous, B Zambrowicz, A Murphy, JC Lin, LE Macdonald, C Daly, M Sleeman, G Thurston, Z Li IL-6-GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models Science Advances, 2023-04-14;9(15):eadf4490. 2023-04-14 [PMID: 37058568] (Bioassay, Mouse) Bioassay Mouse
M Nomura, Y Yokoyama, D Yoshimura, Y Minagawa, A Yamamoto, Y Tanaka, N Sekiguchi, D Marukawa, M Ichihara, H Itakura, K Matsumoto, Y Morimoto, H Tomihara, A Inoue, T Ogino, N Miyoshi, H Takahashi, H Takahashi, M Uemura, S Kobayashi, T Mizushima, T Anada, M Mori, Y Doki, M Tanaka, H Eguchi, H Yamamoto Simple Detection and Culture of Circulating Tumor Cells from Colorectal Cancer Patients Using Poly(2-Methoxyethyl Acrylate)-Coated Plates International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835361] (Bioassay, Human) Bioassay Human
A Filliol, Y Saito, A Nair, DH Dapito, LX Yu, A Ravichandr, S Bhattachar, S Affo, N Fujiwara, H Su, Q Sun, TM Savage, JR Wilson-Kan, JM Caviglia, L Chin, D Chen, X Wang, S Caruso, JK Kang, AD Amin, S Wallace, R Dobie, D Yin, OM Rodriguez-, C Yin, A Mehal, B Izar, RA Friedman, RG Wells, UB Pajvani, Y Hoshida, HE Remotti, N Arpaia, J Zucman-Ros, M Karin, NC Henderson, I Tabas, RF Schwabe Opposing roles of hepatic stellate cell subpopulations in hepatocarcinogenesis Nature, 2022-10-05;0(0):. 2022-10-05 [PMID: 36198802] (Bioassay, Human, Mouse) Bioassay Human, Mouse
MRF Gosselin, V Mournetas, M Borczyk, S Verma, A Occhipinti, J Róg, L Bozycki, M Korostynsk, SC Robson, C Angione, C Pinset, DC Gorecki Loss of full-length dystrophin expression results in major cell-autonomous abnormalities in proliferating myoblasts Elife, 2022-09-27;11(0):. 2022-09-27 [PMID: 36164827] (Bioassay, Mouse) Bioassay Mouse
A Elgaabari, N Imatomi, H Kido, M Seki, S Tanaka, Y Matsuyoshi, T Nakashima, S Sawano, W Mizunoya, T Suzuki, M Nakamura, JE Anderson, R Tatsumi A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF Biochemistry and Biophysics Reports, 2022-06-11;31(0):101295. 2022-06-11 [PMID: 35721345] (Bioassay, Rat) Bioassay Rat
MB Kelley, TJ Geddes, M Ochiai, NM Lampl, WW Kothmann, SR Fierstein, V Kent, K DeCicco-Sk Loss of Tpl2 activates compensatory signaling and resistance to EGFR/MET dual inhibition in v-RAS transduced keratinocytes PLoS ONE, 2022-03-24;17(3):e0266017. 2022-03-24 [PMID: 35325006] (Cell Culture, Mouse) Cell Culture Mouse
TL Nielsen, TM Hornsyld, T Pinós, C Brolin, J Vissing, TO Krag Growth Factors Do Not Improve Muscle Function in Young or Adult mdx Mice Biomedicines, 2022-01-28;10(2):. 2022-01-28 [PMID: 35203514] (In Vivo, Mouse) In Vivo Mouse
A Addante, C González-C, C Roncero, N Lazcanoitu, J García-Sáe, B Herrera, A Sánchez BMP9 Promotes an Epithelial Phenotype and a Hepatocyte-like Gene Expression Profile in Adult Hepatic Progenitor Cells Cells, 2022-01-21;11(3):. 2022-01-21 [PMID: 35159174] (Bioassay, Human) Bioassay Human
Show All 59 Publications.

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Bioinformatics

Gene Symbol Hgf
Uniprot