Recombinant Mouse HGF Protein


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity, Bioactivity

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Recombinant Mouse HGF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 10-30 ng/mL. Measured by its ability to bind Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 7065-ME) in a functional ELISA with an estimated KD <0.2 nM.
Mouse myeloma cell line, NS0-derived mouse HGF protein
Gln33-Arg495 (alpha) & Val496-Leu728 (beta)
Accession #
N-terminal Sequence
No results obtained: Gln33 predicted (alpha) & Val496 (beta)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
53.4 kDa ( alpha -chain), 26 kDa ( beta -chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60 kDa and 33 kDa, reducing conditions
Read Publications using
2207-HG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HGF Protein

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A


HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91% - 95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF‑dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

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Publications for HGF (2207-HG)(41)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Bioassay, Cell Culture, Ex Vivo, In Vivo.

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Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using 2207-HG Applications Species
A Chiche, A Di-Cicco, L Sesma-Sanz, L Bresson, P de la Gran, MA Glukhova, MM Faraldo, MA Deugnier p53 controls the plasticity of mammary luminal progenitor cells downstream of Met signaling Breast Cancer Res., 2019;21(1):13. 2019 [PMID: 30683141] (Bioassay, Mouse) Bioassay Mouse
NF Bonan, D Kowalski, K Kudlac, K Flaherty, JC Gwilliam, LG Falkenberg, E Maradiaga, KL DeCicco-Sk Inhibition of HGF/MET signaling decreases overall tumor burden and blocks malignant conversion in Tpl2-related skin cancer Oncogenesis, 2019;8(1):1. 2019 [PMID: 30631034] (Bioassay, Mouse) Bioassay Mouse
C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA Sci Rep, 2018;8(1):12341. 2018 [PMID: 30120295] (Bioassay, Mouse) Bioassay Mouse
Y Jing, Q Sun, X Xiong, R Meng, S Tang, S Cao, Y Bi, D Zhu Hepatocyte Growth Factor alleviates hepatic insulin resistance and lipid accumulation in high-fat diet-fed mice J Diabetes Investig, 2018;0(0):. 2018 [PMID: 30070033] (In Vivo, Mouse) In Vivo Mouse
JA Zepp, J Zhao, C Liu, K Bulek, L Wu, X Chen, Y Hao, Z Wang, X Wang, W Ouyang, MF Kalady, J Carman, WP Yang, J Zhu, C Blackburn, YH Huang, TA Hamilton, B Su, X Li IL-17A-Induced PLET1 Expression Contributes to Tissue Repair and Colon Tumorigenesis J. Immunol., 2017;0(0):. 2017 [PMID: 29070673] (Bioassay, Mouse) Bioassay Mouse
E Paldy, M Simonetti, T Worzfeld, KK Bali, L Vicuña, S Offermanns, R Kuner Semaphorin 4C Plexin-B2 signaling in peripheral sensory neurons is pronociceptive in a model of inflammatory pain Nat Commun, 2017;8(1):176. 2017 [PMID: 28765520] (In Vivo, Mouse) In Vivo Mouse
SC Shih, TC Ho, SL Chen, YP Tsao Pigment epithelium-derived factor (PEDF) peptide promotes the expansion of hepatic stem/progenitor cells via ERK and STAT3-dependent signaling Am J Transl Res, 2017;9(3):1114-1126. 2017 [PMID: 28386338] (Bioassay, Mouse) Bioassay Mouse
Z Zhang, K Newton, SK Kummerfeld, J Webster, DS Kirkpatric, L Phu, J Eastham-An, J Liu, WP Lee, J Wu, H Li, MR Junttila, VM Dixit Transcription factor Etv5 is essential for the maintenance of alveolar type II cells Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28351980] (Bioassay, Mouse) Bioassay Mouse
K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke BMP-9 interferes with liver regeneration and promotes liver fibrosis Gut, 2017;0(0):. 2017 [PMID: 28336518] (Bioassay, Mouse) Bioassay Mouse
V Kochat, Z Equbal, P Baligar, V Kumar, M Srivastava, A Mukhopadhy JMJD3 aids in reprogramming of bone marrow progenitor cells to hepatic phenotype through epigenetic activation of hepatic transcription factors PLoS ONE, 2017;12(3):e0173977. 2017 [PMID: 28328977] (Bioassay, Mouse) Bioassay Mouse
Show All 41 Publications.

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Gene Symbol Hgf