Recombinant Mouse HGF Propeptide Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse HGF Propeptide Protein, CF Summary

• Details of Functionality: >40 ng of Recombinant Mouse HGF Propeptide is cleaved to release active HGF when treated for 4 hours at 37 °C with 10 ng of kallikrein.
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse HGF protein
  Gln33-Leu728
• Accession #: Q08048
• N-terminal Sequence: No results obtained: Gln33 predicted, sequencing might be blocked
• Protein/Peptide Type: Recombinant Proteins
• Gene: Hgf
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 79.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 84 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HGF Propeptide Protein, CF

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-7). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates an additional HGF isoform with a 5 aa deletion in the first Kringle domain. Mouse Pro‑HGF shares 92%-93% aa sequence identity with bovine, canine, feline, and human Pro-HGF and 99% with rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (8, 9). HGF-dependent c-MET activation is implicated in the development of many human cancers (10). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (11, 12). HGF induces the up-regulation of Integrin  alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (13). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (13, 14). HGF can also alter epithelium morphology by the induction of Nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (15). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (16). HGF promotes the motility of cardiac stem cells in damaged myocardium (17).

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
5. Nakamura, T. et al. (1989) Nature 342:440.
6. Liu, Y. et al. (1993) Biochim. Biophys. Acta 1216:299.
7. Lee, C.C. et al. (1993) Cell Adhes. Commun. 1:101.
8. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
9. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
10. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
11. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
12. Montesano, R. et al. (1991) Cell 67:901.
13. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
14. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
15. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
16. Mineo, R. et al. (1994) Endocrinology 145:4355.
17. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Publications for HGF (7058-HG)(1)

Publication using 7058-HG

• Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS ONE, 2015-05-04;10(5):e0125797. 2015-05-04 [PMID: 25938594] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Hgf
• Uniprot:
  • Q08048()