No results obtained: Gln33 predicted, sequencing might be blocked
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.01 EU per 1 μg of the protein by the LAL method.
79.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
84 kDa, reducing conditions
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HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-7). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an additional HGF isoform with a 5 aa deletion in the first Kringle domain. Mouse Pro‑HGF shares 92%-93% aa sequence identity with bovine, canine, feline, and human Pro-HGF and 99% with rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (8, 9). HGF-dependent c-MET activation is implicated in the development of many human cancers (10). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (11, 12). HGF induces the up-regulation of Integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (13). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (13, 14). HGF can also alter epithelium morphology by the induction of Nectin‑1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (15). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (16). HGF promotes the motility of cardiac stem cells in damaged myocardium (17).
Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
Nakamura, T. et al. (1989) Nature 342:440.
Liu, Y. et al. (1993) Biochim. Biophys. Acta 1216:299.
Lee, C.C. et al. (1993) Cell Adhes. Commun. 1:101.
Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
Corso, S. et al. (2005) Trends Mol. Med. 11:284.
Maeshima, A. et al. (2000) Kid. Int. 58:1511.
Montesano, R. et al. (1991) Cell 67:901.
Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
VEGFR-2 - A highly active kinase VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry... Read full blog post.
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