Recombinant Mouse Glypican 3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Glypican 3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Glypican 3 is coated at 5 μg/mL (100 μL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is found to be approximately 0.75-3.75 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Glypican 3 protein
  Gln25-Met557 & Ser358-Met557, both with a C-terminal 6-His tag
• Accession #: AAH36126
• N-terminal Sequence: Gln25 predicted (No result obtained, sequencing might be blocked) & Ser358
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gpc3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 61.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 66-98 kDa & 30-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Glypican 3 Protein, CF

• DGSX
• Glypican 3
• glypican proteoglycan 3
• glypican-3
• GPC3
• GTR2-2
• heparan sulphate proteoglycan
• Intestinal protein OCI-5
• MXR7
• OCI5
• OCI-5
• secreted glypican-3
• SGB
• SGBS
• SGBS1SDYS

Background

The Glypicans (GPCs) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1 ‑ 4). Glypicans exhibit a 60 ‑ 70 kDa protein core that consists of a globular N‑terminus, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C‑terminal N‑ and O‑linked carbohydrate attachment sites. At least two subfamilies of Glypicans are known (GPC1, 2, 4, and 6 and GPC3 and 5) (1, 5). Mouse Glypican 3 is synthesized as a 579 amino acid (aa) preproprecursor that contains a 24 aa signal sequence, a 535 aa mature segment, and a 20 aa C-terminal prosegment (6 ‑ 8). There are three potential N-linked, and two potential O-linked sites for glycosylation or glycanation. O-linked glycanation utilizes heparan sulfate (HS) and this contributes 60 ‑ 120 kDa to an overall molecular weight of approximately 200 kDa for Glypican 3 (9, 10). Glypican 3 undergoes convertase‑mediated proteolytic processing between Arg357 ‑ Ser358 to generate a disulfide-linked cell-surface heterodimer (9). It may also undergo Notum cleavage of the GPI-anchor to create a soluble circulating form (11, 12). Mature mouse Glypican 3 shares 95% and 99% aa identity with mature human and rat Glypican 3, respectively. Sources for Glypican 3 include liver carcinoma cells plus mesoderm and mesodermal derivatives. Cell types in mice include mesenchyme, prechondrocytes, primitive hematopoietic progenotors, fetal hepatocytes, and pericardium (6, 13). Glypican 3 is best known in human for its association with SGB syndrome, a condition characterized by tissue overgrowth (dysmorphogenesis), potentially through its binding to CD26. Normally, Glypican 3 binds to and down-regulates cell surface CD26.  In the absence of Glypican 3, CD26 promotes cell proliferation, possibly resulting in tissue malformation(s) (14 ‑ 16). Glypican 3 makes use of its both its core protein and HS adduct to impact select growth factor activity. The core protein is known to bind to Wnt and stabilize the Wnt:Frizzled interactions (1, 17, 18). It also binds to hedgehog (HH), serving as a competitive inhibitor for Patched. This results in the internalization and degradation of circulating HH, and it also removes a soluble promoter of Smoothened signaling (1, 19). The HS component of Glypican 3, by contrast, is reported to interact with members of the FGF system, promoting FGF receptor dimerization and signaling (1, 2). Finally, Glypican 3 binds to surface membrane Glut4, facilitating its stabilization and promoting the transport of glucose into the cell (20).

1. Filmis, J. et al. (2008) Genome Biol. 9:224.
2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
3. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
4. Lamoureux, F. et al. (2007) BioEssays 29:758.
5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
6. Pellegrini, M. et al. (1998) Dev. Dyn. 213:431.
7. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
8. Ho, M. and H. Kim (2011) Eur. J. Cancer 47:333.
9. Capurro, M.I. et al. (2005) J. Biol. Chem. 280:41201.
10. Filmus, J. et al. (1995) Biochem. J. 311:561.
11. Traister, A. et al. (2008) Biochem. J. 410:503.
12. Liu, H. et al. (2010) World J. Gastroenterol. 16:4410.
13. Viviano, B.L. et al. (2005) Dev. Biol. 282:152.
14. Gonzales, A.D. et al. (1998) J. Cell Biol. 141:1407.
15. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146:255.
16. Cavoodi, J. et al. (2007) Proteomics 7:2300.
17. Song, H.H. et al. (2005) J. Biol. Chem. 280:2116.
18. Capurro, M.I. et al. (2005) Cancer Res. 65:6245.
19. Capurro, M.I. et al. (2008) Dev. Cell 14:700.
20. Taguchi, A. et al. (2008) Biochem. Biophys. Res. Commun. 369:1204.

Publications for Glypican 3 (6938-GP)(1)

Publication using 6938-GP

• Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K; Identification of the growth cone as a probe and driver of neuronal migration in the injured brain Nature communications 2024-03-09 [PMID: 38461182] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Gpc3
• Uniprot:
  • AAH36126()