Syndecan-1/CD138 Antibody

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Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at ...read more
Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Syndecan-1/CD138 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Gln18-Glu251
Accession # NP_002988
Specificity
Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Syndecan-1/CD138 Antibody (Catalog # AF2780)
ELISA Detection:Human Syndecan-1/CD138 Biotinylated Antibody (Catalog # BAF2780)
Standard: Recombinant Human Syndecan-1/CD138 (Catalog # 2780-SD)
Control
Syndecan-1/CD138 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
AF2780 in the following applications:

Publications
Read Publications using
AF2780 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-1/CD138 Antibody

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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    Publications for Syndecan-1/CD138 Antibody (AF2780)(9)

    We have publications tested in 1 confirmed species: Human.

    We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunodepletion, Immunoprecipitation, Western Blot.


    Filter By Application
    Flow Cytometry
    (1)
    ICC
    (1)
    IHC
    (2)
    IHC-P
    (1)
    Immunodepletion
    (1)
    Immunoprecipitation
    (1)
    Western Blot
    (2)
    All Applications
    Filter By Species
    Human
    (9)
    All Species
    Showing Publications 1 - 9 of 9.
    Publications using AF2780 Applications Species
    MP Garrido, I Hurtado, M Valenzuela, R Salvatierr, A Hernández, M Vega, A Selman, AFG Quest, C Romero NGF-Enhanced Vasculogenic Properties of Epithelial Ovarian Cancer Cells Is Reduced by Inhibition of the COX-2/PGE2 Signaling Axis Cancers (Basel), 2019;11(12):. 2019 [PMID: 31817839] (Western Blot, Human) Western Blot Human
    AM Hoffmann-V, SS Weigt, V Palchevski, E Volkmann, R Saggar, N Li, Ø Midtvedt, MB Lund, T Garen, MC Fishbein, A Ardehali, DJ Ross, T Ueland, P Aukrust, JP Lynch, RM Elashoff, Ø Molberg, JA Belperio Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis PLoS ONE, 2018;13(11):e0206545. 2018 [PMID: 30457999] (IHC-P, Human) IHC-P Human
    S Offenbache, Y Jiao, SJ Kim, J Marchesan, KL Moss, L Jing, K Divaris, S Bencharit, CS Agler, T Morelli, S Zhang, L Sun, WT Seaman, D Cowley, SP Barros, JD Beck, M Munz, AS Schaefer, KE North GWAS for Interleukin-1? levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation Nat Commun, 2018;9(1):3686. 2018 [PMID: 30206230] (IHC, Human) IHC Human
    MA Qasaimeh, YC Wu, S Bose, A Menachery, S Talluri, G Gonzalez, M Fulciniti, JM Karp, RH Prabhala, R Karnik Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device Sci Rep, 2017;7(0):45681. 2017 [PMID: 28374831] (Immunoprecipitation, Human) Immunoprecipitation Human
    Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions. J Biol Chem, 2016;291(4):1652-63. 2016 [PMID: 26601950] (Flow Cytometry, Human) Flow Cytometry Human
    Stewart M, Ramani V, Sanderson R Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk. J Biol Chem, 2015;290(2):941-9. 2015 [PMID: 25404732] (Western Blot, Human) Western Blot Human
    Aragao A, Belloni M, Simabuco F, Zanetti M, Yokoo S, Domingues R, Kawahara R, Pauletti B, Goncalves A, Agostini M, Graner E, Coletta R, Fox J, Leme A Novel processed form of syndecan-1 shed from SCC-9 cells plays a role in cell migration. PLoS ONE, 2012;7(8):e43521. 2012 [PMID: 22905270] (ICC, Human) ICC Human
    Ramani VC, Yang Y, Ren Y Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity. J. Biol. Chem., 2011;286(8):6490-9. 2011 [PMID: 21131364] (Immunodepletion, Human) Immunodepletion Human
    Bahlis NJ, King AM, Kolonias D, Carlson LM, Liu HY, Hussein MA, Terebelo HR, Byrne GE, Levine BL, Boise LH, Lee KP CD28-mediated regulation of multiple myeloma cell proliferation and survival. Blood, 2007;109(11):5002-10. 2007 [PMID: 17311991] (IHC, Human) IHC Human

    Review for Syndecan-1/CD138 Antibody (AF2780) (1) 51

    Average Rating: 5
    (Based on 1 review)
    We have 1 review tested in 1 species: Human.

    Reviews using AF2780:
    Filter by Applications
    IF
    (1)
    All Applications
    Filter by Species
    Human
    (1)
    All Species
    Images Ratings Applications Species Date Details
    Immunofluorescence Syndecan-1/CD138 AF2780
    Enlarge
    5
    reviewed by:
    Natalie Erdmann
    IF Human 03/13/2015
    View

    Summary

    ApplicationImmunofluorescence
    Sample TestedRPMI-8226 multiple myeloma cell line, and Daudi Burkitt lymphoma cell line
    SpeciesHuman

    Blocking

    Blocking DetailsBlocking buffer 10% normal donkey serum/PBS 45 minutes at room temperature

    Primary Anitbody

    Dilution RatioDilution 1:400 (0.5 ug/mL), incubated overnight at 4oC in 10% normal donkey serum/PBS

    Secondary Antibody

    Secondary DescriptionCy3 F(ab')2 donkey anti-goat IgG
    Secondary Manufacturer Cat#Jackson #705-166-147
    Secondary Concentration1:2000 in 10% normal donkey serum/PBS

    Details

    Detection NotesZeiss AxioImager, Cy3 filter cube for 100 ms exposure
    Fixation DetailsImmersion-fixed in 4% paraformaldehyde 10 minutes at room temperature (final concentration 2% PFA); quench 0.66M glycine 45 min
    Wash DescriptionWash 3X 5 minutes with PBS after primary Ab incubation, wash 5X 5 minutes PBS after secondary Ab incubation

    Comments

    CommentsManufacturer's recommended dilution of 5-15 ug/ml MUCH TOO CONCENTRATED for IF/ICC

    Product General Protocols

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    FAQs for Syndecan-1/CD138 Antibody (AF2780) (0)

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    Control Lysate(s)

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Biotin BAF2780

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    Recent Reviews

    5
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    Natalie Erdmann
    03/13/2015
    Application: IF
    Species: Human

    Bioinformatics

    Gene Symbol SDC1
    Uniprot