Recombinant Mouse Galectin-2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Galectin-2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells. The ED50 for this effect is 0.5-2.5 μg/mL. Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 2.5-12.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse Galectin-2 protein
Ser2-Glu130
Accession #
N-terminal Sequence
Ser2
Protein/Peptide Type
Recombinant Proteins
Gene
Lgals2
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12-14 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Galectin-2 Protein, CF

  • Beta-galactoside-binding lectin L-14-II
  • GAL2
  • Gal-2
  • galectin 2
  • Galectin2
  • Galectin-2
  • HL14gal-2
  • Lactose-binding lectin 2
  • lectin, galactoside-binding, soluble, 2
  • LGALS2
  • MGC75071
  • S-Lac lectin 2

Background

Galectin-2 is an approximately 14 kDa, homodimeric carbohydrate-binding protein of the S-type lectin family (1, 2). Like other prototype Galectins, Galectin-2 consists of a single carbohydrate-recognition domain (CRD) (3). Mouse Galectin-2 shares 86% and 66% amino acid sequence identity with rat and human Galectin-2, respectively. Galectin-2 is primarily expressed in the digestive tract and skin of mice (4-6). It can be S-nitrosylated with S-nitrosoglutathione in mouse gastric mucosa (7).
S-nitrosylation prevents Galectin-2 inactivation by hydrogen peroxide, suggesting that this modification protects Galectin-2 function against oxidative stress (8). Galectin-2 appears to have a role in immune responses. Galectin-2 mRNA and protein levels are down-regulated following infection with H. felis (9). Additionally, Galectin-2 treatment has been shown to reduce MHC class I-restricted immune responses and ameliorate acute and chronic experimental colitis in mice by inducing apoptosis of T cells (6, 10). Galectin-2 may also play a role in cancer since circulating levels of human Galectin-2 are elevated in colon and breast cancer patients (11).

  1. Hokama, A. et al. (2008) World J. Gastroenterol. 14:5133.
  2. Barondes, S.H. et al. (1994) Cell 76:597.
  3. Hirabayashi, J. and K. Kasai (1993) Glycobiology 3:297.
  4. Nio, J. et al. (2005) J. Histochem. Cytochem. 53:1323.
  5. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
  6. Loser, K. et al. (2009) J. Immunol. 182:5419.
  7. Ohtake, K. et al. (2009) J. Proteomics 72:750.
  8. Tamura, M. et al. (2015) Biochem. Biophys. Res. Commun. 457:712.
  9. Takaishi, S. and T.C. Wang (2007) Cancer Sci. 98:284.
  10. Paclik, D. et al. (2008) J. Mol. Med. 86:1395.
  11. Chen, C. et al. (2014) Br. J. Cancer 110:741.

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Bioinformatics

Gene Symbol Lgals2
Uniprot