Proteasome 20S alpha 6 Antibody (OTI4C9)

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Western Blot: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Proteasome 20S alpha 6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; ...read more
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Flow Cytometry: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Proteasome 20S ...read more
Western Blot: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S alpha 6 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 6 Antibody (4C9) [NBP2-03836] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 20S alpha 6 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
OTI4C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Proteasome 20S alpha 6 Antibody (OTI4C9) Summary

Immunogen
Full length human recombinant protein of human PSMA6 (NP_002782) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PSMA6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proteasome 20S alpha 6 Antibody (OTI4C9)

  • EC 3.4.25.1
  • IOTA
  • Macropain iota chain
  • macropain subunit iota
  • MGC22756
  • MGC2333
  • MGC23846
  • Multicatalytic endopeptidase complex iota chain
  • p27K
  • p27KPROS-27
  • PROS27
  • PROS-27
  • PROS2727 kDa prosomal protein
  • prosomal P27K protein
  • proteasome (prosome, macropain) subunit, alpha type, 6
  • Proteasome 20S alpha 6
  • Proteasome iota chain
  • proteasome subunit alpha type-6
  • proteasome subunit iota
  • PSMA6

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMA6
Entrez