FLAP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Flow Cytometry: FLAP Antibody [NB300-891] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Antibody at 6 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FLAP Antibody Summary

Immunogen
Peptide with sequence C-KTISTTISPLLLIP corresponding to C-Terminus according to NP_001620.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALOX5AP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6 ug/mL
  • Peptide ELISA Detection limit 1:6000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30735559). Please note that the current batch of this antibody is not validated for use in WB. Publications citing use in WB were using previous batches.
Control
FLAP Overexpression Lysate
Publications
Read Publications using
NB300-891 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27198524). Rat reactivity reported in scientific literature (PMID: 30735559).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FLAP Antibody

  • arachidonate 5-lipoxygenase-activating protein
  • FLAPMK-886-binding protein

Background

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-58748
Species: Hu, Mu
Applications: ICC/IF, KD, Simple Western, WB
NB110-58748
Species: Hu, Mu
Applications: ICC/IF, KD, Simple Western, WB
211-TBB/CF
Species: Hu
Applications: BA
NBP1-47829
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP1-69315
Species: Hu
Applications: WB
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-94035
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
MAB1684
Species: Hu
Applications: WB
NBP2-25196
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, In vitro, WB
DVE00
Species: Hu
Applications: ELISA
DY3667
Species: Mu
Applications: ELISA
NBP2-24686
Species: Bv, Hu, Mu, Ma-Op, Pm, Rt
Applications: IHC, IHC-P, WB
H00023471-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP1-32550
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-85500
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-59438
Species: Hu
Applications: ELISA, ICC/IF, WB
NLS1317
Species: Hu, Pm, Pm
Applications: Flow, IHC, IHC-P

Publications for FLAP Antibody (NB300-891)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB300-891 Applications Species
Helgadottir A, Manolescu A, Thorleifsson G et al. The gene encoding 5-lipoxygenase activating protein confers risk of myocardial infarction and stroke. Nat Genet 2004 Mar [PMID: 14770184]
Huang YH. Impact of the arachidonic acid/leukotriene signaling pathway for vasculogenesis and leukocyte differentiation of mouse embryonic stem cells. thesis. 2015 Oct 26 (WB, Mouse) WB Mouse
Schmider Ab, Bauer Nc, Sunwoo H et Al. Two and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils J. Biol. Chem. Mar 9 2020 [PMID: 32152223]
SchUtz B, Ruppert AL, Strobel O et al. Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract Sci Rep Nov 25 2019 [PMID: 31767912] (ICC/IF, Human) ICC/IF Human
Schmider AB, Vaught M, Bauer NC, Elliott HL. The organization of leukotriene biosynthesis on the nuclear envelope revealed by single molecule localization microscopy and computational analyses bioRxiv Jul 11 1905 [PMID: 30735559] (ICC/IF, Rat) ICC/IF Rat
Montoro D T, Haber A L et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature Jan 8 2018 [PMID: 30069044] (IHC, Mouse) IHC Mouse
Huang YH, Sharifpanah F, Becker S et al. Impact of Arachidonic Acid and the Leukotriene Signaling Pathway on Vasculogenesis of Mouse Embryonic Stem Cells. Cells Tissues Organs. May 31 2016 [PMID: 27198524] (WB, Mouse) WB Mouse
Finkensieper A, Kieser S, Bekhite MM et al. The 5-lipoxygenase pathway regulates vasculogenesis in differentiating mouse embryonic stem cells. Cardiovasc Res. 2010 Apr 1 [PMID: 19965960] (WB, Mouse)

Details:
BLT1 (IMG-71194) WB (mouse embryonic stem cell).
WB Mouse

Reviews for FLAP Antibody (NB300-891) (0)

There are no reviews for FLAP Antibody (NB300-891). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for FLAP Antibody (NB300-891) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FLAP Products

Bioinformatics Tool for FLAP Antibody (NB300-891)

Discover related pathways, diseases and genes to FLAP Antibody (NB300-891). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FLAP Antibody (NB300-891)

Discover more about diseases related to FLAP Antibody (NB300-891).
 

Pathways for FLAP Antibody (NB300-891)

View related products by pathway.

PTMs for FLAP Antibody (NB300-891)

Learn more about PTMs related to FLAP Antibody (NB300-891).
 

Research Areas for FLAP Antibody (NB300-891)

Find related products by research area.

Blogs on FLAP

There are no specific blogs for FLAP, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FLAP Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ALOX5AP
Entrez
Uniprot