FLAP Antibody

Images

 
Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Staining of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Staining of Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FLAP Antibody Summary

Immunogen
Peptide with sequence C-KTISTTISPLLLIP corresponding to C-Terminus according to NP_001620.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALOX5AP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB successful usage in transfected HeLa is reported in scientific literature (PMID: 18692027). Use in ICC/IF reported in scientific literature (PMID: 30735559). Use in Western Blot reported in scientific literature (PMID:27198524).
Control
FLAP Overexpression Lysate
Publications
Read Publications using
NB300-891 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27198524). Rat reactivity reported in scientific literature (PMID: 30735559).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FLAP Antibody

  • arachidonate 5-lipoxygenase-activating protein
  • FLAPMK-886-binding protein

Background

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FLAP Antibody (NB300-891)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB300-891 Applications Species
Schmider Ab, Bauer Nc, Sunwoo H et Al. Two and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils J. Biol. Chem. Mar 9 2020 [PMID: 32152223]
SchUtz B, Ruppert AL, Strobel O et al. Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract Sci Rep Nov 25 2019 [PMID: 31767912] (ICC/IF, Human) ICC/IF Human
Finkensieper A, Kieser S, Bekhite MM et al. The 5-lipoxygenase pathway regulates vasculogenesis in differentiating mouse embryonic stem cells. Cardiovasc Res. 2010 Apr 1 [PMID: 19965960] (WB, Mouse)

Details:
BLT1 (IMG-71194) WB (mouse embryonic stem cell).
WB Mouse
Helgadottir A, Manolescu A, Thorleifsson G et al. The gene encoding 5-lipoxygenase activating protein confers risk of myocardial infarction and stroke. Nat Genet 2004 Mar [PMID: 14770184]
Huang YH. Impact of the arachidonic acid/leukotriene signaling pathway for vasculogenesis and leukocyte differentiation of mouse embryonic stem cells. thesis. 2015 Oct 26 (WB, Mouse) WB Mouse
Schmider AB, Vaught M, Bauer NC, Elliott HL. The organization of leukotriene biosynthesis on the nuclear envelope revealed by single molecule localization microscopy and computational analyses bioRxiv Jul 11 1905 [PMID: 30735559] (ICC/IF, Rat) ICC/IF Rat
Montoro D T, Haber A L et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature Jan 8 2018 [PMID: 30069044] (IHC, Mouse) IHC Mouse
Huang YH, Sharifpanah F, Becker S et al. Impact of Arachidonic Acid and the Leukotriene Signaling Pathway on Vasculogenesis of Mouse Embryonic Stem Cells. Cells Tissues Organs. May 31 2016 [PMID: 27198524] (WB, Mouse) WB Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FLAP Antibody (NB300-891) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALOX5AP
Entrez
Uniprot