Human Galectin-10 ELISA Kit (Colorimetric)

ELISA: Human Galectin-10 ELISA Kit (Colorimetric) [NBP2-75319] - Samples were spiked with high concentrations of Human Galectin-10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Galectin-10 ELISA Kit (Colorimetric) [NBP2-75319] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Galectin-10 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CLC in samples. No significant cross-reactivity or interference between Human CLC and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.11%
• Intra-Assay: CV% < 5.21%
• Spike Recovery: 88-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CLC

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Galectin-10 ELISA Kit (Colorimetric)

• Charcot-Leyden crystal proteinLGALS10A
• CLC
• EC 3.1.1.5
• eosinophil lysophospholipase
• GAL10
• Gal-10
• galectin 10
• Galectin10
• Galectin-10
• LGALS10
• LPPL_HUMAN
• Lysolecithin acylhydrolase
• MGC149659

Background

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Galectin-10 ELISA Kit (NBP2-75319)(1)

Publication using NBP2-75319

• Weng K, Chien K, Huang S et al. iTRAQ Proteomics Identified the Potential Biomarkers of Coronary Artery Lesion in Kawasaki Disease and In Vitro Studies Demonstrated That S100A4 Treatment Made HCAECs More Susceptible to Neutrophil Infiltration International Journal of Molecular Sciences 2022-10-23 [PMID: 36361563] (ELISA)

Review for Galectin-10 ELISA Kit (NBP2-75319) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: CM Martin 02/28/2025

Summary

• Lot: WV23T2ZL2030

Comments

• Comments: The standard curve was good but we did not detect a signal on protein extracts from cells or from FFPE tissue.

FAQs for Galectin-10 ELISA Kit (NBP2-75319). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CLC