Recombinant Mouse FGF basic/FGF2/bFGF Protein

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1 μg/lane of Recombinant Mouse FGF basic/FGF2/bFGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Recombinant Mouse FGF basic/FGF2/bFGF (Catalog # 3139-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.3-1.8 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse FGF basic/FGF2/bFGF Protein Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.3-1.8 ng/mL.
Source
E. coli-derived mouse FGF basic/FGF2/bFGF protein
Ala11-Ser154
Accession #
N-terminal Sequence
Ala11
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
3139-FB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF basic/FGF2/bFGF Protein

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • Prostatropin

Background

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation (1-3). Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4). Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH-3T3 cells (5). The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic (6, 7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3). Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions (1-3).

  1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
  2. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
  3. Presta, M. et al. (2005) Cytokine. Growth Factor Rev. 16:159.
  4. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
  5. Quarto, N. et al. (2005) Gene 356:49.
  6. Tsuneto, M. et al. (2005) Biochem. Biophys. Res. Comm. 335:1239.
  7. Hebert, J. M. et al. (1990) Dev. Biol. 138:454.

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Publications for FGF basic/FGF2/bFGF (3139-FB)(138)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, In vivo.


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Bioassay
(114)
Cell Culture
(8)
In Vivo
(13)
In vivo
(2)
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Human
(2)
Mouse
(133)
Rat
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 138. Show All 138 Publications.
Publications using 3139-FB Applications Species
JM Kim, YS Yang, KH Park, X Ge, R Xu, N Li, M Song, H Chun, S Bok, JF Charles, O Filhol-Coc, B Boldyreff, T Dinter, PB Yu, N Kon, W Gu, T Takarada, MB Greenblatt, JH Shim A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation Nat Commun, 2020;11(1):2289. 2020 [PMID: 32385263] (Bioassay, Mouse) Bioassay Mouse
M Chen, P Lu, Q Ma, Y Cao, N Chen, W Li, S Zhao, B Chen, J Shi, Y Sun, H Shen, L Sun, J Shen, Q Liao, Y Zhang, J Hong, W Gu, R Liu, G Ning, W Wang, J Wang CTNNB1/&amp;beta-catenin dysfunction contributes to adiposity by regulating the cross-talk of mature adipocytes and preadipocytes Sci Adv, 2020;6(2):eaax9605. 2020 [PMID: 31934629] (Cell Culture, Mouse) Cell Culture Mouse
H Li, J Li, R Han, X Deng, J Shi, H Huang, N Hamad, A McCaughley, J Liu, C Wang, K Chen, D Wei, J Qiang, S Thatcher, Y Wu, C Liu, O Thibault, X Wei, S Chen, H Qian, BP Zhou, P Xu, XH Yang Deletion of tetraspanin CD151 alters the Wnt oncogene-induced mammary tumorigenesis: A cell type-linked function and signaling Neoplasia, 2019;21(12):1151-1163. 2019 [PMID: 31783316] (Bioassay, Mouse) Bioassay Mouse
M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors Stem Cell Reports, 2019;13(6):1053-1067. 2019 [PMID: 31708478] (Cell Culture, Mouse) Cell Culture Mouse
OA Bahri, N Naldaiz-Ga, DC Kennedy, AM Wheatley, A Izeta, KJA McCullagh The panniculus carnosus muscle: A novel model of striated muscle regeneration that exhibits sex differences in the mdx mouse Sci Rep, 2019;9(1):15964. 2019 [PMID: 31685850] (Bioassay, Mouse) Bioassay Mouse
X Yuan, M Liu, X Cao, S Yang Ciliary IFT80 regulates dental pulp stem cells differentiation by FGF/FGFR1 and Hh/BMP2 signaling Int. J. Biol. Sci., 2019;15(10):2087-2099. 2019 [PMID: 31592124] (Bioassay, Mouse) Bioassay Mouse
Y Rong, W Liu, C Lv, J Wang, Y Luo, D Jiang, L Li, Z Zhou, W Zhou, Q Li, G Yin, L Yu, J Fan, W Cai Neural stem cell small extracellular vesicle-based delivery of 14-3-3t reduces apoptosis and neuroinflammation following traumatic spinal cord injury by enhancing autophagy by targeting Beclin-1 Aging (Albany NY), 2019;11(18):7723-7745. 2019 [PMID: 31563124] (Bioassay, Mouse) Bioassay Mouse
L Vanheer, J Song, N De Geest, A Janiszewsk, I Talon, C Provenzano, T Oh, J Chappell, V Pasque Tox4 modulates cell fate reprogramming J. Cell. Sci., 2019;0(0):. 2019 [PMID: 31519808] (Bioassay, Mouse) Bioassay Mouse
R Bartz, K Fukuchi, T Ohtsuka, T Lange, K Gruner, I Watanabe, S Hayashi, Y Oda, R Kawaida, H Komori, Y Kashimoto, P Wirtz, JA Mayer, M Redondo-Mü, S Saito, M Takahashi, H Hanzawa, E Imai, A Martinez, M Hanai, D Häussinger, RW Chapman, T Agatsuma, J Bange, R Abraham Pre-clinical development of U3-1784, a novel FGFR4 antibody against cancer, and avoidance of its on-target toxicity Mol. Cancer Ther., 2019;0(0):. 2019 [PMID: 31350344] (Bioassay, Mouse) Bioassay Mouse
D Cabello-Ri, H Sarmiento-, J López-Barn, AM Muñoz-Cabe Mitochondrial Complex I Function Is Essential for Neural Stem/Progenitor Cells Proliferation and Differentiation Front Neurosci, 2019;13(0):664. 2019 [PMID: 31297047] (Bioassay, Mouse) Bioassay Mouse
Show All 138 Publications.

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FAQs for FGF basic/FGF2/bFGF (3139-FB). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol Fgf2
Uniprot