Recombinant Mouse Ephrin-B3 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Ephrin-B3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Ephrin-B3 Fc Chimera is coated at 2 μg/mL, Recombinant Mouse EphB3 Fc Chimera (Catalog # 432-B3) binds with an apparent K_D <1 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Ephrin-B3 protein
  

  Mouse Ephrin-B3 (Leu28-Ala227) Accession # O35393	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: O35393
• N-terminal Sequence: Leu28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Efnb3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 58-61 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-B3 Fc Chimera Protein, CF

• EFL-6
• Efnb3
• Elk-L3
• EPH-related receptor tyrosine kinase ligand 8
• Ephrin B3
• EphrinB3
• Ephrin-B3
• Epl8
• EPLG8EPH-related receptor transmembrane ligand ELK-L3
• LERK-8
• LERK8EFL6
• NLERK-2

Background

Ephrin-B3, also known as Elk-L3, LERK8, Eplg8, NLERK-2, and EFL6, is an approximately 50 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B3 preferentially interacts with receptors in the EphB family and also with EphA4. The binding of Ephrin-B3 to Eph proteins also triggers reverse signaling through Ephrin-B3 (1, 2). Mature mouse Ephrin-B3 consists of a 200 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 92 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B3 shares 96% and 98% aa sequence identity with human and rat Ephrin-B3, respectively. Ephrin-B3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord (5-9). It is up-regulated in glioma and promotes tumor cell invasion and migration (10). Ephrin-B3 functions as a repulsive axon guidance molecule by inducing growth cone collapse, neurite retraction, and axon pruning (5-8). Its repulsive effect along the spinal cord midline restricts motor neuron axons to their ipsilateral sides, thereby maintaining the independence of voluntary left side/right side movements (8, 9). Ephrin-B3 plays a role in the regulation of excitatory synapse density and synaptic maturation (6, 11, 12). It also functions as a cellular receptor for Nipah virus (13) and can induce the migration of memory B cells (14).

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
3. Tang, X.X. et al. (1997) Genomics 41:17.
4. Bergemann, A.D. et al. (1998) Oncogene 16:471.
5. Benson, M.D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10694.
6. Xu, N.-J. et al. (2011) Nat. Neurosci. 14:1421.
7. Xu, N.-J. and M. Henkemeyer (2009) Nat. Neurosci. 12:268.
8. Kullander, K. et al. (2001) Genes Dev. 15:877.
9. Yokoyama, N. et al. (2001) Neuron 29:85.
10. Nakada, M. et al. (2006) Cancer Res. 66:8492.
11. McClelland, A.C. et al. (2010) Proc. Natl. Acad. Sci. USA 107:8830.
12. Antion, M.D. et al. (2010) Mol. Cell. Neurosci. 45:378.
13. Negrete, O.A. et al. (2006) PLoS Pathog. 2:e7.
14. Holen, H.L. et al. (2011) Scand. J. Immunol. 74:144.

Publications for Ephrin-B3 (7655-EB)(5)

Publications using 7655-EB

• N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature Communications, 2022-10-13;13(1):6040. 2022-10-13 [PMID: 36229455] (Bioassay, Mouse)
• M Yang, W Zhu, T Truong, B Pickering, S Babiuk, D Kobasa, L Banadyga Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays Viruses, 2022-07-28;14(8):. 2022-07-28 [PMID: 36016279] (ELISA Capture, Mouse)
• L Schoonaert, L Rué, B Roucourt, M Timmers, S Little, L Chávez-Gut, M Dewilde, P Joyce, A Curnock, P Weber, J Haustraete, G Hassanzade, B De Stroope, L Van Den Bo, P Van Damme, R Lemmens, W Robberecht Identification and characterization of Nanobodies targeting the EphA4 receptor J. Biol. Chem., 2017-05-19;292(27):11452-11465. 2017-05-19 [PMID: 28526745] (Bioassay, Mouse)
• Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Mouse)
• Liu Q, Bradel-Tretheway B, Monreal A, Saludes J, Lu X, Nicola A, Aguilar H Nipah virus attachment glycoprotein stalk C-terminal region links receptor binding to fusion triggering. J Virol, 2014-11-26;89(3):1838-50. 2014-11-26 [PMID: 25428863] (Bioassay, Virus)

Bioinformatics

• Gene Symbol: Efnb3
• Uniprot:
  • O35393()