Recombinant Mouse EphB4 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse EphB4 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphB4 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin‑B2 Fc Chimera (Catalog # 496-EB) with a linear range of 0.078-5 ng/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse EphB4 protein
  

  Mouse EphB4 (Leu16-Ala539) Accession # P54761	DIEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P54761
• N-terminal Sequence: Leu16
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ephb4
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 85.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphB4 Fc Chimera Protein, CF

• EC 2.7.10
• EC 2.7.10.1
• EPH receptor B4
• EphB4
• ephrin type-B receptor 4
• hepatoma transmembrane kinase
• Htk
• HTKephrin receptor EphB4
• Mdk2
• Myk1
• soluble EPHB4 variant 1
• soluble EPHB4 variant 2
• soluble EPHB4 variant 3
• Tyro11
• Tyrosine-protein kinase receptor HTK
• Tyrosine-protein kinase TYRO11

Background

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin-B2 andephrin-B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Publications for EphB4 (446-B4)(27)

Publications using 446-B4

• Stewen, J;Kruse, K;Godoi-Filip, AT;Zenia, ;Jeong, HW;Adams, S;Berkenfeld, F;Stehling, M;Red-Horse, K;Adams, RH;Pitulescu, ME; Eph-ephrin signaling couples endothelial cell sorting and arterial specification Nature communications 2024-04-03 [PMID: 38570531] (Bioassay, Human)
• S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse)
• D Bissen, MK Kracht, F Foss, J Hofmann, A Acker-Palm EphrinB2 and GRIP1 stabilize mushroom spines during denervation-induced homeostatic plasticity Cell Reports, 2021-03-30;34(13):108923. 2021-03-30 [PMID: 33789115] (Bioassay, Mouse)
• Tun Wang, Jia Liu, Haiyang Liu, Shin-Rong Lee, Luis Gonzalez, Jolanta Gorecka et al. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae Journal of Surgical Research 2021-06-01 [PMID: 33039109] (Bioassay, In vivo assay, Mouse)
• YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo Oxid Med Cell Longev, 2020-06-05;2020(0):1404915. 2020-06-05 [PMID: 32587656] (Bioassay, Mouse)
• Y Yoon, G Voloudakis, N Doran, E Zhang, C Dimovasili, L Chen, Z Shao, S Darmanis, C Tang, J Tang, VX Wang, PR Hof, NK Robakis, A Georgakopo PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival Mol. Psychiatry, 2020-06-15;0(0):. 2020-06-15 [PMID: 32541930] (Bioassay, Mouse)
• W Wang, C Yuan, T Geng, Y Liu, S Zhu, C Zhang, Z Liu, P Wang EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling Stem Cell Res Ther, 2020-01-29;11(1):40. 2020-01-29 [PMID: 31996240] (Cell Culture, Human)
• E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development Elife, 2019-12-23;8(0):. 2019-12-23 [PMID: 31868584] (Bioassay, Mouse)
• A Grandi, I Zini, S Palese, C Giorgio, M Tognolini, F Marchesani, S Bruno, L Flammini, AM Cantoni, R Castelli, A Lodola, A Fusari, E Barocelli, S Bertoni Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD Front Pharmacol, 2019-06-17;10(0):691. 2019-06-17 [PMID: 31297055] (ELISA Capture, Mouse)
• D Herrero, S Cañón, G Albericio, RM Carmona, S Aguilar, S Mañes, A Bernad Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart Redox Biol, 2019-03-04;22(0):101156. 2019-03-04 [PMID: 30851670] (Bioassay, Flow Cytometry, Mouse)
• J Yoon, YS Hwang, M Lee, J Sun, HJ Cho, L Knapik, IO Daar TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration Nat Commun, 2018-08-28;9(1):3491. 2018-08-28 [PMID: 30154457] (Bioassay, Xenopus)
• JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling Exp. Mol. Med., 2018-04-27;50(4):50. 2018-04-27 [PMID: 29700355] (Bioassay, Mouse)
• NA Warren, G Voloudakis, Y Yoon, NK Robakis, A Georgakopo The product of the ?-secretase processing of ephrinB2 regulates VE-cadherin complexes and angiogenesis Cell. Mol. Life Sci., 2018-02-10;0(0):. 2018-02-10 [PMID: 29428965] (Bioassay, Bovine)
• A Ghori, FB Freimann, M Nieminen-K, I Kremenetsk, K Gertz, M Endres, P Vajkoczy EphrinB2 Activation Enhances Vascular Repair Mechanisms and Reduces Brain Swelling After Mild Cerebral Ischemia Arterioscler. Thromb. Vasc. Biol, 2017-03-02;0(0):. 2017-03-02 [PMID: 28254815] (In Vivo, Mouse)
• Greenblatt M, Park K, Oh H, Kim J, Shin D, Lee J, Lee J, Singh A, Lee K, Hu D, Xiao C, Charles J, Penninger J, Lotinun S, Baron R, Ghosh S, Shim J CHMP5 controls bone turnover rates by dampening NF-kappaB activity in osteoclasts. J Exp Med, 2015-07-20;212(8):1283-301. 2015-07-20 [PMID: 26195726] (Bioassay, Mouse)
• Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun, 2015-03-26;6(0):6576. 2015-03-26 [PMID: 25807892] (Bioassay, Human)
• White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, Srivastava D Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells, 2013-01-01;31(1):92-103. 2013-01-01 [PMID: 23079999] (Flow Cytometry, Human)
• Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Nature, 2010-05-05;465(7297):487-91. 2010-05-05 [PMID: 20445540] (Bioassay, ICC, Immunoprecipitation, Mouse)
• Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth. Blood, 2009-07-13;114(9):1803-12. 2009-07-13 [PMID: 19597185] (Bioassay, In Vivo, Human)
• Narazaki G, Uosaki H, Teranishi M, Okita K, Kim B, Matsuoka S, Yamanaka S, Yamashita JK Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells. Circulation, 2008-07-14;118(5):498-506. 2008-07-14 [PMID: 18625891] (ICC, Mouse)
• Masuda J, Usui R, Maru Y Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis. J. Biol. Chem., 2008-02-28;283(19):13148-55. 2008-02-28 [PMID: 18308734] (Bioassay, Human)
• Bouzioukh F, Wilkinson GA, Adelmann G, Frotscher M, Stein V, Klein R Tyrosine phosphorylation sites in ephrinB2 are required for hippocampal long-term potentiation but not long-term depression. J. Neurosci., 2007-10-17;27(42):11279-88. 2007-10-17 [PMID: 17942722] (Bioassay, Mouse)
• Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway. Biochem. Biophys. Res. Commun., 2004-10-08;323(1):17-23. 2004-10-08 [PMID: 15351694] (Bioassay, Human)
• Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia, 2004-05-01;6(3):248-57. 2004-05-01 [PMID: 15153337] (Bioassay, Human)
• Noren NK, Lu M, Freeman AL, Koolpe M, Pasquale EB Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth. Proc. Natl. Acad. Sci. U.S.A., 2004-04-05;101(15):5583-8. 2004-04-05 [PMID: 15067119] (Bioassay, Human)
• le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A Flow regulates arterial-venous differentiation in the chick embryo yolk sac. Development, 2003-12-17;131(2):361-75. 2003-12-17 [PMID: 14681188] (IHC, In Vivo, Avian - Quail, Chicken)
• Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J. Cell. Sci., 2003-05-06;116(0):2461-70. 2003-05-06 [PMID: 12734395] (Bioassay, ICC, IHC, Human)

Bioinformatics

• Gene Symbol: Ephb4
• Uniprot:
  • P54761()