Recombinant Mouse Endocan Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Endocan Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Recombinant Mouse Endocan/ESM-1 immobilized at 25 μg/mL, 100 μL/well, will mediate >20% cell adhesion (100 μL/well at 0.5 x 10⁶ cells/mL).
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse Endocan/ESM-1 protein
  Trp20-Arg184, with a C-terminal 10-His tag
• Accession #: Q9QYY7
• N-terminal Sequence: Trp20
• Protein/Peptide Type: Recombinant Proteins
• Gene: Esm1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 21-25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Endocan Protein, CF

• Endocan
• endothelial cell-specific molecule 1
• ESM1
• ESM-1
• IGFBP-rp6

Background

Endocan (endothelial cell proteoglycan; also known as endothelial‑cell specific molecule‑1 [ESM‑1]), is a 50 kDa, monomeric, secreted, cysteine‑rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N‑terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N‑linked glycosylation sites. Based on human Endocan, there are at least two potential O‑linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4‑O sulfated N‑acetyl galactosamine with alpha ‑iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF‑ alpha and VEGF, (1, 7) and is known to bind to LFA‑1 ( alpha _L beta ₂) on the surface of PBMCs, blocking LFA‑1 interaction with ICAM‑1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).

1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
2. Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
3. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
4. Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
5. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
6. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
7. Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.

Publications for Endocan/ESM-1 (1999-EC)(1)

Publication using 1999-EC

• Kumar, SK;Mani, KP; Proinflammatory signaling mechanism of endocan in macrophages: Involvement of TLR2 mediated MAPK-NFkB pathways Cytokine 2023-12-29 [PMID: 38159469] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Esm1
• Uniprot:
  • Q9QYY7()