Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of human neutrophils. The ED50 for this effect is 0.5-3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD90/Thy1 protein
Mouse CD90/Thy1
(Gln20-Lys130)
Accession # P01831
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained, Gln20 predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Thy1
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
40 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-60 kDa, reducing conditions
Publications
Read Publication using
7335-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen
  • Thy1

Background

CD90, also known as Thy1, is an approximately 25-35 kDa glycoprotein that mediates cellular adhesion and exerts wide ranging effects in different tissues (1, 2). Mature mouse CD90 contains one immunoglobulin-like domain followed by a GPI anchor (3-5). CD90 is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells (6-8). Its species-specific expression in lymphoid cells allows its use as a thymocyte and pan T cell marker in mouse but not in human (1). CD90 can form dimers and higher order multimers (9). It binds to heparin, the proteoglycan Syndecan-4, and Integrins alpha M beta 2, alpha V beta 3, and  alpha V beta 5 (8, 10-13). Through these interactions, CD90 inhibits neurite outgrowth (7), promotes astrocyte adhesion (10), mediates the adhesion and extravasation of leukocytes and melanoma cells (8, 12), supports the Thrombospondin-1 induced disassembly of fibroblast focal adhesions (14), and inhibits the activation of latent TGF-beta 1, myofibroblast differentiation, and lung fibrosis (13)
  1. Haeryfar, S.M.M. and D.W. Hoskin (2004) J. Immunol. 173:3581.
  2. Rege, T.A. and J.S. Hagood (2006) Biochim. Biophys. Acta 1763:991.
  3. Chang, H.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:3819.
  4. Giguere, V. et al. (1985) EMBO J. 4:2017.
  5. Thomas, P.M. and L.E. Samelson (1992) J. Biol. Chem. 267:12317.
  6. Craig, W. et al. (1993) J. Exp. Med. 177:1331.
  7. Tiveron, M-C. et al. (1992) Nature 355:745.
  8. Wetzel, A. et al. (2004) J. Immunol. 172:3850.
  9. Mahanthappa, N.K. and P.H. Patterson (1992) Dev. Biol. 150:60.
  10. Avalos, A.M. et al. (2009) J. Cell Sci. 122:3462.
  11. Hueber, A.O. et al. (1992) J. Immunol. 148:3692.
  12. Saalbach, A. et al. (2005) Oncogene 24:4710.
  13. Zhou, Y. et al. (2010) J. Biol. Chem. 285:22382.
  14. Barker, T.H. et al. (2004) J. Biol. Chem. 279:23510.

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7335-CD
Species: Mu
Applications: Bioactivity

Publications for CD90/Thy1 (7335-CD)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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(1)
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FAQs for CD90/Thy1 (7335-CD). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.

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Bioinformatics

Gene Symbol Thy1
Uniprot