Recombinant Mouse CCL5/RANTES Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse CCL5/RANTES Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 1.5‑9 ng/mL. Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 30‑100 ng/mL.
Source
E. coli-derived mouse CCL5/RANTES protein
Ser24-Ser91
Accession #
N-terminal Sequence
Ser24
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl5
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
478-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL5/RANTES Protein

  • CCL5
  • chemokine (C-C motif) ligand 5
  • D17S136Enormally T-expressed, and presumably secreted
  • EoCP
  • Eosinophil chemotactic cytokine
  • RANTES
  • SISd
  • SIS-delta
  • small inducible cytokine A5 (RANTES)
  • small inducible cytokine subfamily A (Cys-Cys), member 5
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • T-cell specific protein p288
  • TCP228T-cell-specific protein RANTES

Background

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% aa sequence identity with rat CCL5 and 75% - 88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).
  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Heeger, P. et al. (1992) Kidney Int. 41:220.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

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Publications for CCL5/RANTES (478-MR)(13)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(8)
ELISA Standard
(2)
In Vivo
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(10)
N/A
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 478-MR Applications Species
JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura The HIV co-receptor CCR5 regulates osteoclast function Nat Commun, 2017;8(1):2226. 2017 [PMID: 29263385] (Bioassay, Mouse) Bioassay Mouse
N Suffee, C Le Visage, H Hlawaty, R Aid-Launai, V Vanneaux, J Larghero, O Haddad, O Oudar, N Charnaux, A Sutton Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy Sci Rep, 2017;7(1):13294. 2017 [PMID: 29038476] (In Vivo, Mouse) In Vivo Mouse
Y Pan, LJ Smithson, Y Ma, D Hambardzum, DH Gutmann Ccl5 establishes an autocrine high-grade glioma growth regulatory circuit critical for mesenchymal glioblastoma survival Oncotarget, 2017;0(0):. 2017 [PMID: 28380429] (Bioassay, Mouse) Bioassay Mouse
SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan P2X7 receptor-dependent tuning of gut epithelial responses to infection Immunol. Cell Biol, 2017;95(2):178-188. 2017 [PMID: 27559003] (Bioassay, Mouse) Bioassay Mouse
Costa M, de Negreiros C, Bornstein V, Valente R, Mengel J, Henriques M, Benjamim C, Penido C Murine IL-17+ Vgamma4 T lymphocytes accumulate in the lungs and play a protective role during severe sepsis. BMC Immunol, 2015;16(0):36. 2015 [PMID: 26037291] (Bioassay, Mouse) Bioassay Mouse
Sottnik J, Dai J, Zhang H, Campbell B, Keller E Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases. Cancer Res, 2015;75(11):2151-8. 2015 [PMID: 25855383] (Bioassay, Human) Bioassay Human
Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J KIM-1-mediated phagocytosis reduces acute injury to the kidney. J Clin Invest, 2015;125(4):1620-36. 2015 [PMID: 25751064] (ELISA Standard, Mouse) ELISA Standard Mouse
Sato S, Zhang X The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney. Clin Exp Immunol, 2014;177(1):102-9. 2014 [PMID: 24580413] (Bioassay, Mouse) Bioassay Mouse
Kimura, Kenichi, Nagano, Masumi, Salazar, Georgina, Yamashita, Toshihar, Tsuboi, Ikki, Mishima, Hajime, Matsushita, Shonosuk, Sato, Fujio, Yamagata, Kenji, Ohneda, Osamu The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions. Stem Cells Dev, 2014;23(5):488-501. 2014 [PMID: 24171667] (In Vivo, Mouse) In Vivo Mouse
Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5). J Bone Miner Res, 2013;28(10):2070-80. 2013 [PMID: 23553711] (Bioassay, Mouse) Bioassay Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol Ccl5
Entrez
Uniprot