Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is 40-160 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human VE-Cadherin protein
Human VE-Cadherin
(Asp48-Gln593)
Accession # P33151
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp48
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CDH5
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
89 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
116 kDa, reducing conditions
Publications
Read Publications using
938-VC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin

Background

VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins (1). Mature human VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca++-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).
  1. Giannotta, M. et al. (2013) Dev. Cell 26:441.
  2. Breviario, F. et al. (1995) Arterioscler. Throm. Vasc. Biol. 15:1229.
  3. Geyer, H. et al. (1999) Glycobiology 9:915.
  4. Herren, B. et al. (1998) Mol. Biol. Cell 9:1589.
  5. Li, H. et al. (2010) Cancer Gene Ther. 17:700.
  6. Breier, G. et al. (1996) Blood 87:630.
  7. Ema, M. et al. (2006) Blood 108:4018.
  8. Oberlin, E. et al. (2010) Blood 116:4444.
  9. Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
  10. Perryn, E.D. et al. (2008) Dev. Biol. 313:545.
  11. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
  12. Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703.
  13. Taddei, A. et al. (2008) Nat. Cell Biol. 10:923.
  14. Luo, Y. and G.L. Radice (2005) J. Cell Biol. 169:29.
  15. Rudini, N. et al. (2008) EMBO J. 27:993.
  16. Soeki, T. et al. (2004) Circ. J. 68:1.
  17. Sulkowska, M. et al. (2006) Tumori 92:67.
  18. Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.

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Bioinformatics

Gene Symbol CDH5
Entrez
Uniprot