Recombinant Human TrkB Fc Chimera Protein, CF


Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.1-0.4 μg/mL in the more
1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively more

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TrkB Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
Mouse myeloma cell line, NS0-derived human TrkB protein
Accession # Q16620.1
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
71.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
120 kDa-130 kDa, reducing conditions
Read Publications using
688-TK in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TrkB Fc Chimera Protein, CF

  • BDNF/NT-3 growth factors receptor
  • EC 2.7.10
  • EC
  • GP145-TrkB
  • Neurotrophic tyrosine kinase receptor type 2
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • TrkB tyrosine kinase
  • TrkB
  • trk-B
  • TRKBBDNF-tropomyosine receptor kinase B
  • tyrosine kinase receptor B


The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.

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Publications for TrkB (688-TK)(54)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bacteria, Chicken, Xenograft, Xenopus.

We have publications tested in 7 applications: Bioassay, Cell Culture, Immunoprecipitation, In Vivo, Neutralization, PcIP, Surface Plasmon Resonance.

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Surface Plasmon Resonance
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Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using 688-TK Applications Species
Moliner, R;Girych, M;Brunello, CA;Kovaleva, V;Biojone, C;Enkavi, G;Antenucci, L;Kot, EF;Goncharuk, SA;Kaurinkoski, K;Kuutti, M;Fred, SM;Elsilä, LV;Sakson, S;Cannarozzo, C;Diniz, CRAF;Seiffert, N;Rubiolo, A;Haapaniemi, H;Meshi, E;Nagaeva, E;Öhman, T;Róg, T;Kankuri, E;Vilar, M;Varjosalo, M;Korpi, ER;Permi, P;Mineev, KS;Saarma, M;Vattulainen, I;Casarotto, PC;Castrén, E; Psychedelics promote plasticity by directly binding to BDNF receptor TrkB Nature neuroscience 2023-06-01 [PMID: 37280397] (Bioassay, Mouse) Bioassay Mouse
E Kutsarova, A Schohl, M Munz, A Wang, YY Zhang, OM Bilash, ES Ruthazer BDNF signaling in correlation-dependent structural plasticity in the developing visual system PloS Biology, 2023-04-03;21(4):e3002070. 2023-04-03 [PMID: 37011100] (In Vivo, Xenopus) In Vivo Xenopus
BM Balog, K Deng, T Askew, B Hanzlicek, M Kuang, MS Damaser Brain-Derived Neurotrophic Factor Is Indispensable to Continence Recovery after a Dual Nerve and Muscle Childbirth Injury Model International Journal of Molecular Sciences, 2023-03-05;24(5):. 2023-03-05 [PMID: 36902428] (Bioassay, Rat) Bioassay Rat
MV Pavon, S Navakkode, LW Wong, S Sajikumar Inhibition of Nogo-A rescues synaptic plasticity and associativity in APP/PS1 animal model of Alzheimer&#039;s disease Seminars in cell & developmental biology, 2022-04-14;0(0):. 2022-04-14 [PMID: 35431138] (Bioassay, Mouse) Bioassay Mouse
J Zhou, Y Geng, T Su, Q Wang, Y Ren, J Zhao, C Fu, M Weber, H Lin, JS Kaminker, N Liu, M Sheng, Y Chen NMDA receptor-dependent prostaglandin-endoperoxide synthase 2 induction in neurons promotes glial proliferation during brain development and injury Cell Reports, 2022-03-29;38(13):110557. 2022-03-29 [PMID: 35354047] (Bioassay, Mouse) Bioassay Mouse
A Petkova-Tu, N Gödecke, J Viotti, M Korte, T Dresbach Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling Bmc Biology, 2021-09-27;19(1):215. 2021-09-27 [PMID: 34579720] (Cell Culture, Human) Cell Culture Human
PM Horvath, NL Chanaday, B Alten, ET Kavalali, LM Monteggia A subthreshold synaptic mechanism regulating BDNF expression and resting synaptic strength Cell Reports, 2021-08-03;36(5):109467. 2021-08-03 [PMID: 34348149] (Bioassay, Rat) Bioassay Rat
Y Zhang, PD Smolen, LJ Cleary, JH Byrne Quantitative description of the interactions among kinase cascades underlying long-term plasticity of Aplysia sensory neurons Scientific Reports, 2021-07-22;11(1):14931. 2021-07-22 [PMID: 34294802] (Neutralization, Human) Neutralization Human
Y Lin, M Yao, H Wu, F Wu, S Cao, H Ni, J Dong, D Yang, Y Sun, X Kou, J Li, H Xiao, L Chang, J Wu, Y Liu, C Luo, D Zhu Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery Theranostics, 2021-01-27;11(8):3760-3780. 2021-01-27 [PMID: 33664860] (Bioassay, Mouse) Bioassay Mouse
AG González, OM Lazo, FC Bronfman The Rab5-Rab11 endosomal pathway is required for BDNF-induced CREB transcriptional regulation in hippocampal neurons J. Neurosci., 2020-09-14;0(0):. 2020-09-14 [PMID: 32928890] (Bioassay, Rat) Bioassay Rat
Show All 54 Publications.

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Blogs on TrkB.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Gene Symbol NTRK2