Recombinant Human TrkB Fc Chimera Protein, CF

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1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively ...read more
Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.1-0.4 μg/mL in the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human TrkB Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
Source
Mouse myeloma cell line, NS0-derived human TrkB protein
HumanTrkB
(Cys32-His430)
Accession # Q16620.1
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Cys32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NTRK2
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
71.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa-130 kDa, reducing conditions
Publications
Read Publications using
688-TK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TrkB Fc Chimera Protein, CF

  • BDNF/NT-3 growth factors receptor
  • EC 2.7.10
  • EC 2.7.10.1
  • GP145-TrkB
  • Neurotrophic tyrosine kinase receptor type 2
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • TrkB tyrosine kinase
  • TrkB
  • trk-B
  • TRKBBDNF-tropomyosine receptor kinase B
  • tyrosine kinase receptor B

Background

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.

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688-TK
Species: Hu
Applications: Bioactivity

Publications for TrkB (688-TK)(49)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bacteria, Chicken, N/A.

We have publications tested in 7 applications: Bioassay, Cell Culture, In Vivo, In vivo, Neutralization, PcIP, Surface Plasmon Resonance.


Filter By Application
Bioassay
(22)
Cell Culture
(2)
In Vivo
(19)
In vivo
(1)
Neutralization
(1)
PcIP
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(10)
Rat
(26)
Bacteria
(1)
Chicken
(1)
N/A
(3)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using 688-TK Applications Species
A Petkova-Tu, N Gödecke, J Viotti, M Korte, T Dresbach Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling Bmc Biology, 2021;19(1):215. 2021 [PMID: 34579720] (Cell Culture, Human) Cell Culture Human
PM Horvath, NL Chanaday, B Alten, ET Kavalali, LM Monteggia A subthreshold synaptic mechanism regulating BDNF expression and resting synaptic strength Cell Reports, 2021;36(5):109467. 2021 [PMID: 34348149] (Bioassay, Rat) Bioassay Rat
Y Zhang, PD Smolen, LJ Cleary, JH Byrne Quantitative description of the interactions among kinase cascades underlying long-term plasticity of Aplysia sensory neurons Scientific Reports, 2021;11(1):14931. 2021 [PMID: 34294802] (Neutralization, Human) Neutralization Human
Y Lin, M Yao, H Wu, F Wu, S Cao, H Ni, J Dong, D Yang, Y Sun, X Kou, J Li, H Xiao, L Chang, J Wu, Y Liu, C Luo, D Zhu Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery Theranostics, 2021;11(8):3760-3780. 2021 [PMID: 33664860] (Bioassay, Mouse) Bioassay Mouse
AG González, OM Lazo, FC Bronfman The Rab5-Rab11 endosomal pathway is required for BDNF-induced CREB transcriptional regulation in hippocampal neurons J. Neurosci., 2020;0(0):. 2020 [PMID: 32928890] (Bioassay, Rat) Bioassay Rat
X Li, Q Wang, J Ding, S Wang, C Dong, Q Wu Exercise training modulates glutamic acid decarboxylase-65/67 expression through TrkB signaling to ameliorate neuropathic pain in rats with spinal cord injury Mol Pain, 2020;16(0):1744806920924. 2020 [PMID: 32418502] (In Vivo, Rat) In Vivo Rat
A Patnaik, E Spiombi, A Frasca, N Landsberge, M Zagrebelsk, M Korte Fingolimod Modulates Dendritic Architecture in a BDNF-Dependent Manner Int J Mol Sci, 2020;21(9):. 2020 [PMID: 32349283] (Cell Culture, Mouse) Cell Culture Mouse
G Fulgenzi, Z Hong, F Tomassoni-, LF Barella, J Becker, C Barrick, D Swing, S Yanpallewa, BS Croix, J Wess, O Gavrilova, L Tessarollo Novel metabolic role for BDNF in pancreatic &amp;beta-cell insulin secretion Nat Commun, 2020;11(1):1950. 2020 [PMID: 32327658] (Bioassay, Mouse) Bioassay Mouse
LE Montroull, V Danelon, AB Cragnolini, DH Mascó Loss of TrkB Signaling Due to Status Epilepticus Induces a proBDNF-Dependent Cell Death Front Cell Neurosci, 2019;13(0):4. 2019 [PMID: 30800056] (In Vivo, Rat) In Vivo Rat
JM Hall, F Gomez-Pini, LM Savage Nerve Growth Factor Is Responsible for Exercise-Induced Recovery of Septohippocampal Cholinergic Structure and Function Front Neurosci, 2018;12(0):773. 2018 [PMID: 30443202] (In vivo, Rat) In vivo Rat
Show All 49 Publications.

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Blogs on TrkB.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Bioinformatics

Gene Symbol NTRK2
Uniprot