Recombinant Human TrkB Fc Chimera Protein, CF

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1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively.
Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 0.1-0.4 μg/mL in the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human TrkB Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.
Source
Mouse myeloma cell line, NS0-derived human TrkB protein
HumanTrkB
(Cys32-His430)
Accession # Q16620.1
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Cys32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NTRK2
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
71.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa-130 kDa, reducing conditions
Publications
Read Publications using
688-TK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TrkB Fc Chimera Protein, CF

  • BDNF/NT-3 growth factors receptor
  • EC 2.7.10
  • EC 2.7.10.1
  • GP145-TrkB
  • Neurotrophic tyrosine kinase receptor type 2
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • TrkB tyrosine kinase
  • TrkB
  • trk-B
  • TRKBBDNF-tropomyosine receptor kinase B
  • tyrosine kinase receptor B

Background

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

  1. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  2. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
  3. Soppet, D. et al. (1991) Cell 65:895.

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Publications for TrkB (688-TK)(35)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bacteria, Chicken, N/A.

We have publications tested in 4 applications: Bioassay, In Vivo, PcIP, Surface Plasmon Resonance.


Filter By Application
Bioassay
(16)
In Vivo
(15)
PcIP
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(7)
Rat
(17)
Bacteria
(1)
Chicken
(1)
N/A
(3)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using 688-TK Applications Species
A Suzuki, D Matsuura, H Kanatani, S Yano, M Tsunakawa, S Matsuyama, H Shigemori Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth Biol. Pharm. Bull., 2017;40(10):1784-1788. 2017 [PMID: 28966252] (Bioassay, Human) Bioassay Human
JI Hurtado-Za, B Ramachandr, S Ahmed, R Halder, C Bolleyer, A Awasthi, MA Stahlberg, RJ Wagener, K Anderson, RM Drenan, HA Lester, JM Miwa, JF Staiger, A Fischer, C Dean TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus Nat Commun, 2017;8(0):15878. 2017 [PMID: 28722015] (Bioassay, Mouse) Bioassay Mouse
M Sharma, T Dierkes, S Sajikumar Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons Aging Cell, 2017;0(0):. 2017 [PMID: 28665013] (Bioassay, N/A) Bioassay N/A
LF Martins, RO Costa, JR Pedro, P Aguiar, SC Serra, FG Teixeira, N Sousa, AJ Salgado, RD Almeida Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF Sci Rep, 2017;7(1):4153. 2017 [PMID: 28646200] (Bioassay, Human) Bioassay Human
RR Nair, S Patil, A Tiron, T Kanhema, D Panja, L Schiro, K Parobczak, G Wilczynski, CR Bramham Dynamic Arc SUMOylation and Selective Interaction with F-Actin-Binding Protein Drebrin A in LTP Consolidation In Vivo Front Synaptic Neurosci, 2017;9(0):8. 2017 [PMID: 28553222] (In Vivo, Rat) In Vivo Rat
Y Guo, ZJ Su, YK Chen, Z Chai BDNF/NT3 Regulate AIS Location and Affect Neuronal Excitability in Cultured Hippocampal Neurons J. Neurochem., 2017;0(0):. 2017 [PMID: 28440877] (Bioassay, Rat) Bioassay Rat
JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28351971] (Bioassay, N/A) Bioassay N/A
Potentiation of Synaptic GluN2B NMDAR Currents by Fyn Kinase Is Gated through BDNF-Mediated Disinhibition in Spinal Pain Processing Cell Rep, 2016;17(10):2753-2765. 2016 [PMID: 27926876] (Bioassay, Rat) Bioassay Rat
James W Grau Complete spinal cord injury (SCI) transforms how brain derived neurotrophic factor (BDNF) affects nociceptive sensitization Exp. Neurol., 2016;288(0):38-50. 2016 [PMID: 27818188] (In Vivo, Rat) In Vivo Rat
Yoshinori Hayashi BK channels in microglia are required for morphine-induced hyperalgesia Nat Commun, 2016;7(0):11697. 2016 [PMID: 27241733] (In Vivo, Mouse) In Vivo Mouse
Show All 35 Publications.

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Blogs on TrkB.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Bioinformatics

Gene Symbol NTRK2
Entrez
Uniprot