Recombinant Human TGF-beta 1 Protein

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Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh) TGF-beta 1 (Catalog # 240-B). Control cells were ...read more
1 μg/lane of Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human TGF-beta 1 Protein Summary

Additional Information
A New TGF-beta 1 is Available! The new protein is Hek293 expressed!
New Product 7754-BH
Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.
The specific activity of Recombinant Human TGF-beta 1 is approximately 2.5 x 104 U/μg, which is calibrated against human TGF‑ beta 1 Standard (NIBSC code: 89/514).
Source
Chinese Hamster Ovary cell line, CHO-derived
Ala279-Ser390
Accession #
N-terminal Sequence
Ala279
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
240-B in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 1 Protein

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

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Publications for (412)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Feline, Mink, N/A, Porcine, Rabbit.

We have publications tested in 7 applications: Bioassay, ELISA Standard, ELISA development, In Vivo, Standard, WB Ctrl, cell culture.


Filter By Application
Bioassay
(375)
ELISA Standard
(6)
ELISA development
(1)
In Vivo
(15)
Standard
(1)
WB Ctrl
(4)
cell culture
(2)
All Applications
Filter By Species
Human
(233)
Mouse
(128)
Rat
(23)
Bovine
(1)
Canine
(2)
Chicken
(1)
Equine
(3)
Feline
(2)
Mink
(1)
N/A
(9)
Porcine
(1)
Rabbit
(3)
All Species
Showing Publications 1 - 10 of 412. Show All 412 Publications.
Publications using 240-B Applications Species
M Sato-Matsu, T Matsubara, A Daikoku, Y Okina, L Longato, K Rombouts, LTT Thuy, J Adachi, T Tomonaga, K Ikeda, K Yoshizato, M Pinzani, N Kawada Fibroblast growth factor 2 (FGF2) regulates cytoglobin expression and activation of human hepatic stellate cells via JNK signaling J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28916723] (Bioassay, Human) Bioassay Human
Y Qi, N Wang, Y He, J Zhang, H Zou, W Zhang, W Gu, Y Huang, X Lian, J Hu, J Zhao, X Cui, L Pang, F Li Transforming growth factor-?1 signaling promotes epithelial-mesenchymal transition-like phenomena, cell motility, and cell invasion in synovial sarcoma cells PLoS ONE, 2017;12(8):e0182680. 2017 [PMID: 28829837] (Bioassay, Human) Bioassay Human
VC Rodríguez-, P Martínez-R, F Català-Mol, J Rodríguez-, A Garcia-Gom, G Poorani-Su, L Ciudad, H Hernando, A Pérez-Garc, C Company, JM Urquiza, AR Ramiro, JM Di Noia, A Vaquero, E Ballestar Activation-induced cytidine deaminase targets SUV4-20-mediated histone H4K20 trimethylation to class-switch recombination sites Sci Rep, 2017;7(1):7594. 2017 [PMID: 28790320] (Bioassay, Mouse) Bioassay Mouse
S Lee, D Kim, J Kang, E Kim, W Kim, H Youn, B Youn Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production Cell. Physiol. Biochem., 2017;42(4):1684-1700. 2017 [PMID: 28743125]
M Renard, L Muiño-Mosq, EC Manalo, S Tufa, EJ Carlson, DR Keene, J De Backer, LY Sakai Sex, pregnancy and aortic disease in Marfan syndrome PLoS ONE, 2017;12(7):e0181166. 2017 [PMID: 28708846] (Bioassay, Human) Bioassay Human
H Kamata, Y Tsukasaki, T Sakai, R Ikebe, J Wang, A Jeffers, J Boren, S Owens, T Suzuki, M Higashihar, S Idell, TA Tucker, M Ikebe KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis Sci Rep, 2017;7(1):4556. 2017 [PMID: 28676645] (Bioassay, Human) Bioassay Human
JC Cheng, CP Tseng, MH Liao, CY Peng, JS Yu, PH Chuang, JT Huang, JJW Chen Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes Sci Rep, 2017;7(1):4448. 2017 [PMID: 28667290] (Bioassay, Human) Bioassay Human
C Caër, C Rouault, T Le Roy, C Poitou, J Aron-Wisne, A Torcivia, JC Bichet, K Clément, M Guerre-Mil, S André Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue Sci Rep, 2017;7(1):3000. 2017 [PMID: 28592801] (Bioassay, Human) Bioassay Human
JB Koo, MO Nam, Y Jung, J Yoo, DH Kim, G Kim, SJ Shin, KM Lee, KB Hahm, JW Kim, SP Hong, KJ Lee, JH Yoo Anti-fibrogenic effect of PPAR-? agonists in human intestinal myofibroblasts BMC Gastroenterol, 2017;17(1):73. 2017 [PMID: 28592228] (Bioassay, Human) Bioassay Human
TP Nguyen, SF Sieg TGF-? inhibits IL-7-induced proliferation in memory but not naive human CD4(+) T cells J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28588029] (Bioassay, Human) Bioassay Human
Show All 412 Publications.

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TGF-beta 1 - a versatile signaling molecule with roles in development and disease
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Bioinformatics

Gene Symbol TGFB1
Entrez
Uniprot