Recombinant Human TGF-beta 1 Protein, CF

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Equivalent bioactivity of CHO-derived (Catalog # 240-B/CF) and HEK293-derived (7754-BH) Recombinant Human TGF‑ beta 1 as measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cell. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TGF-beta 1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.0400-0.200 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein
Ala279-Ser390
Accession #
N-terminal Sequence
Ala279
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
240-B/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 2 µg vials at 20 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Reconstitute 10 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 1 Protein, CF

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGF beta
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Publications for TGF-beta 1 (240-B/CF)(827)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Feline, Fish, Mink, Porcine, Rabbit, Transgenic Mouse, Virus.

We have publications tested in 12 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Capture, ELISA Standard, IHC-P, In Vivo, Stimulation, Surface Plasmon Resonance (SPR, Tissue Culture, Western Blot.


Filter By Application
Bioassay
(727)
Cell Culture
(51)
Differentiation
(4)
ELISA (Standard)
(7)
ELISA Capture
(1)
ELISA Standard
(2)
IHC-P
(1)
In Vivo
(17)
Stimulation
(6)
Surface Plasmon Resonance (SPR
(1)
Tissue Culture
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(540)
Mouse
(223)
Rat
(43)
Bovine
(1)
Canine
(7)
Chicken
(2)
Equine
(4)
Feline
(3)
Fish
(1)
Mink
(2)
Porcine
(4)
Rabbit
(4)
Transgenic Mouse
(4)
Virus
(1)
All Species
Showing Publications 1 - 10 of 827. Show All 827 Publications.
Publications using 240-B/CF Applications Species
Ma, HY;Li, Q;Wong, WR;N'Diaye, EN;Caplazi, P;Bender, H;Huang, Z;Arlantico, A;Jeet, S;Wong, A;Emson, C;Brightbill, H;Tam, L;Newman, R;Roose-Girma, M;Sandoval, W;Ding, N; LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lung Science advances 2023-05-26 [PMID: 37235663] (Bioassay, Human) Bioassay Human
Min, K;Yenilmez, B;Kelly, M;Echeverria, D;Elleby, M;Lifshitz, LM;Raymond, N;Tsagkaraki, E;Harney, SM;DiMarzio, C;McHugh, N;Bramato, B;Morrision, B;Rothstein, JD;Khvorova, A;Czech, MP; Lactate transporter MCT1 in hepatic stellate cells promotes fibrotic collagen expression in nonalcoholic steatohepatitis bioRxiv : the preprint server for biology 2023-05-03 [PMID: 37205462] (Bioassay, Human) Bioassay Human
O Anannya, W Huang, A August ITK signaling regulates a switch between T helper 17 and T regulatory cell lineages via a calcium-mediated pathway bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03 [PMID: 37066370] (Bioassay, Mouse) Bioassay Mouse
N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Journal of dental sciences, 2022-09-16;18(2):567-576. 2022-09-16 [PMID: 37021273] (Cell Culture, Human) Cell Culture Human
N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Journal of dental sciences, 2023;18(2):567-576. 2023 [PMID: 37021273] (Cell Culture, Human) Cell Culture Human
J Sidhaye, P Trepte, N Sepke, M Novatchkov, M Schutzbier, G Dürnberger, K Mechtler, JA Knoblich Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids Elife, 2023-03-29;12(0):. 2023-03-29 [PMID: 36989136] (Bioassay, Human) Bioassay Human
AM Refaat, M Nakata, A Husain, H Kosako, T Honjo, NA Begum HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression Cell Reports, 2023-03-20;42(3):112284. 2023-03-20 [PMID: 36943867] (Bioassay, Mouse) Bioassay Mouse
Z Wang, J Lauko, AW Kijas, EP Gilbert, P Turunen, R Yegappan, D Zou, J Mata, AE Rowan Snake venom-defined fibrin architecture dictates fibroblast survival and differentiation Nature Communications, 2023-02-23;14(1):1029. 2023-02-23 [PMID: 36823141] (Bioassay, Human) Bioassay Human
S Matsuda, A Revandkar, TD Dubash, A Ravi, BS Wittner, M Lin, R Morris, R Burr, H Guo, K Seeger, A Szabolcs, D Che, L Nieman, GA Getz, DT Ting, MS Lawrence, J Gainor, DA Haber, S Maheswaran TGF-beta in the microenvironment induces a physiologically occurring immune-suppressive senescent state Cell Reports, 2023-02-22;42(3):112129. 2023-02-22 [PMID: 36821441] (Bioassay, Human) Bioassay Human
Y Horii, S Matsuda, C Toyota, T Morinaga, T Nakaya, S Tsuchiya, M Ohmuraya, T Hironaka, R Yoshiki, K Kasai, Y Yamauchi, N Takizawa, A Nagasaka, A Tanaka, H Kosako, M Nakaya VGLL3 is a mechanosensitive protein that promotes cardiac fibrosis through liquid-liquid phase separation Nature Communications, 2023-02-08;14(1):550. 2023-02-08 [PMID: 36754961] (Bioassay, Rat) Bioassay Rat