Recombinant Human NKp46/NCR1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human NKp46/NCR1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind Recombinant Human Vimentin (Catalog # 2105-VI) in a functional ELISA with an estimated KD < 2 nM. Garg, A. et al. (2006) J. Immunol. 177:6192.
Source
Mouse myeloma cell line, NS0-derived human NKp46/NCR1 protein
Human NKp46
(Gln22 - Asn254)
Accession #AAH64806
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NCR1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
53 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-80 kDa, reducing conditions
Publications
Read Publications using
1850-NK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKp46/NCR1 Fc Chimera Protein, CF

  • CD335 antigen
  • CD335
  • hNKp46
  • Ly94
  • LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
  • Lymphocyte antigen 94 homolog
  • MAR-1
  • natural cytotoxicity triggering receptor 1
  • Natural killer cell p46-related protein
  • NCR1
  • NK cell-activating receptor
  • NKp46
  • NKP46FLJ99094
  • NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Background

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5 - 7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
  4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  5. Arnon, T. et al. (2004) Blood 103:664.
  6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  7. Mandelboim, O. et al. (2001) Nature 409:1055.
  8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.

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Publications for NKp46/NCR1 (1850-NK)(13)

We have publications tested in 2 confirmed species: Human, Bacteria.

We have publications tested in 3 applications: Bioassay, EnzAct, Flow.


Filter By Application
Bioassay
(2)
EnzAct
(1)
Flow
(10)
All Applications
Filter By Species
Human
(12)
Bacteria
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 1850-NK Applications Species
T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara Frequency and role of NKp46 and NKG2A in hepatitis B virus infection PLoS ONE, 2017;12(3):e0174103. 2017 [PMID: 28328926] (Flow,, Human) Flow Human
Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation Proc Natl Acad Sci USA, 2016;113(21):E2973-82. 2016 [PMID: 27162345] (EnzAct, Human) EnzAct Human
Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Onfelt B Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J Immunol, 2015;195(7):3374-81. 2015 [PMID: 26320254] (Flow, Human) Flow Human
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015;10(5):e0125544. 2015 [PMID: 25950680] (Flow, Human) Flow Human
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014;192(11):5332-42. 2014 [PMID: 24790151] (Bioassay, Human) Bioassay Human
Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013;9(4):e1003257. 2013 [PMID: 23592985] (Bioassay, Human) Bioassay Human
Reiners K, Kessler J, Sauer M, Rothe A, Hansen H, Reusch U, Hucke C, Kohl U, Durkop H, Engert A, von Strandmann E Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients. Mol Ther, 2013;21(4):895-903. 2013 [PMID: 23459515] (Flow, Human) Flow Human
Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells. J. Clin. Invest., 2012;122(7):2369-83. 2012 [PMID: 22684105] (Flow, Human) Flow Human
Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog., 2012;8(1):e1002486. 2012 [PMID: 22253598] (Flow, Human) Flow Human
Esin S, Batoni G, Counoupas C, Stringaro A, Brancatisano FL, Colone M, Maisetta G, Florio W, Arancia G, Campa M Direct binding of human NK cell natural cytotoxicity receptor NKp44 to the surfaces of mycobacteria and other bacteria. Infect. Immun., 2008;76(4):1719-27. 2008 [PMID: 18212080] (Flow, Bacteria) Flow Bacteria
Show All 13 Publications.

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Bioinformatics

Gene Symbol NCR1
Uniprot