Recombinant Human NKp44 Fc Chimera Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human NKp44 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind HeLa human cervical epithelial carcinoma cells in a flow cytometry assay. When 400 ng of rhNKp44 Fc chimera is added to 2 x 105 Hela cells, >25% cells bind to the protein.
Mouse myeloma cell line, NS0-derived human NKp44/NCR2 protein
Human NKp44
(Gln22 - Pro190)
Accession # CAB31968.1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
45 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
61-67 kDa, reducing conditions
Read Publications using
2249-NK in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKp44 Fc Chimera Protein, CF

  • CD336 antigen
  • CD336
  • LY95
  • LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)
  • lymphocyte antigen 95 (activating NK-receptor; NK-p44)
  • Lymphocyte antigen 95 homolog
  • natural cytotoxicity triggering receptor 2
  • Natural killer cell p44-related protein
  • NCR2
  • NK cell activating receptor (NKp44)
  • NK cell-activating receptor
  • NKp44
  • NK-p44dJ149M18.1


NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
  5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.

Publications for NKp44/NCR2 (2249-NK)(15)

We have publications tested in 4 confirmed species: Human, Bacteria, Bacteria - E. Coli, C. albicans.

We have publications tested in 4 applications: Binding Assay, Bioassay, Enzyme Assay, Flow Cytometry.

Filter By Application
Binding Assay
Enzyme Assay
Flow Cytometry
All Applications
Filter By Species
Bacteria - E. Coli
C. albicans
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 2249-NK Applications Species
Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma Translational Oncology, 2022-08-20;25(0):101521. 2022-08-20 [PMID: 35998437] (Flow Cytometry, Human) Flow Cytometry Human
SA Lim, Y Moon, MH Shin, TJ Kim, S Chae, C Yee, D Hwang, H Park, KM Lee Hypoxia-Driven HIF-1&amp;alpha Activation Reprograms Pre-Activated NK Cells towards Highly Potent Effector Phenotypes via ERK/STAT3 Pathways Cancers, 2021-04-15;13(8):. 2021-04-15 [PMID: 33920906] (Bioassay, Human) Bioassay Human
A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function BMC Immunol., 2019-01-29;20(1):8. 2019-01-29 [PMID: 30696399] (Flow Cytometry, Human) Flow Cytometry Human
Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation Proc Natl Acad Sci USA, 2016-05-09;113(21):E2973-82. 2016-05-09 [PMID: 27162345] (Enzyme Assay, Human) Enzyme Assay Human
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015-05-07;10(5):e0125544. 2015-05-07 [PMID: 25950680] (Flow Cytometry, Human) Flow Cytometry Human
Mou , Xiao, Zhou , Yuepeng, Jiang , Peng, Zhou , Tong, Jiang , Qian, Xu , Chengche, Liu , Hongli, Zheng , Tingting, Yuan , Guoyue, Zhang , Yanyun, Chen , Deyu, Mao , Chaoming The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients. Sci Rep, 2014-08-20;4(0):6138. 2014-08-20 [PMID: 25138242] (Bioassay, Human) Bioassay Human
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-04-30;192(11):5332-42. 2014-04-30 [PMID: 24790151] (Bioassay, Human) Bioassay Human
Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013-04-04;9(4):e1003257. 2013-04-04 [PMID: 23592985] (Bioassay, Human) Bioassay Human
Varchetta S, Brunetta E, Roberto A, Mikulak J, Hudspeth K, Mondelli M, Mavilio D Engagement of Siglec-7 receptor induces a pro-inflammatory response selectively in monocytes. PLoS ONE, 2012-09-28;7(9):e45821. 2012-09-28 [PMID: 23029261] (Binding Assay, Bacteria - E. Coli, C. albicans) Binding Assay Bacteria - E. Coli, C. albicans
Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells. J. Clin. Invest., 2012-06-11;122(7):2369-83. 2012-06-11 [PMID: 22684105] (Flow Cytometry, Human) Flow Cytometry Human
Show All 15 Publications.

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Gene Symbol NCR2