Recombinant Human NKp44 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human NKp44 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind HeLa human cervical epithelial carcinoma cells in a flow cytometry assay. When 400 ng of rhNKp44 Fc chimera is added to 2 x 105 Hela cells, >25% cells bind to the protein.
Source
Mouse myeloma cell line, NS0-derived human NKp44/NCR2 protein
Human NKp44
(Gln22 - Pro190)
Accession # O95944
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NCR2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
45 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
61-67 kDa, reducing conditions
Publications
Read Publications using
2249-NK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKp44 Fc Chimera Protein, CF

  • CD336 antigen
  • CD336
  • LY95
  • LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)
  • lymphocyte antigen 95 (activating NK-receptor; NK-p44)
  • Lymphocyte antigen 95 homolog
  • natural cytotoxicity triggering receptor 2
  • Natural killer cell p44-related protein
  • NCR2
  • NK cell activating receptor (NKp44)
  • NK cell-activating receptor
  • NKp44
  • NK-p44dJ149M18.1

Background

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56dim CD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56bright CD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Cantoni, C. et al. (1999) J. Exp. Med. 189:787.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Vitale, M. et al. (1998) J. Exp. Med. 187:2065.
  5. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  6. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.

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2249-NK
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Publications for NKp44/NCR2 (2249-NK)(12)

We have publications tested in 3 confirmed species: Human, Bacteria, C. albicans.

We have publications tested in 4 applications: Binding Assay, Bioassay, EnzAct, Flow.


Filter By Application
Binding Assay
(1)
Bioassay
(3)
EnzAct
(1)
Flow
(7)
All Applications
Filter By Species
Human
(10)
Bacteria
(1)
C. albicans
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 2249-NK Applications Species
Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation Proc Natl Acad Sci USA, 2016;113(21):E2973-82. 2016 [PMID: 27162345] (EnzAct, Human) EnzAct Human
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015;10(5):e0125544. 2015 [PMID: 25950680] (Flow, Human) Flow Human
Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients. Sci Rep, 2014;4(0):6138. 2014 [PMID: 25138242] (Bioassay, Human) Bioassay Human
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014;192(11):5332-42. 2014 [PMID: 24790151] (Bioassay, Human) Bioassay Human
Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013;9(4):e1003257. 2013 [PMID: 23592985] (Bioassay, Human) Bioassay Human
Varchetta S, Brunetta E, Roberto A, Mikulak J, Hudspeth K, Mondelli M, Mavilio D Engagement of Siglec-7 receptor induces a pro-inflammatory response selectively in monocytes. PLoS ONE, 2012;7(9):e45821. 2012 [PMID: 23029261] (Binding Assay, C. albicans) Binding Assay C. albicans
Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells. J. Clin. Invest., 2012;122(7):2369-83. 2012 [PMID: 22684105] (Flow, Human) Flow Human
Madrid, Alexis S, Ganem, Don Kaposi&#039;s sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol, 2012;86(16):8693-704. 2012 [PMID: 22674989] (Flow, Human) Flow Human
Esin S, Batoni G, Counoupas C, Stringaro A, Brancatisano FL, Colone M, Maisetta G, Florio W, Arancia G, Campa M Direct binding of human NK cell natural cytotoxicity receptor NKp44 to the surfaces of mycobacteria and other bacteria. Infect. Immun., 2008;76(4):1719-27. 2008 [PMID: 18212080] (Flow, Bacteria) Flow Bacteria
Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood, 2007;111(3):1309-17. 2007 [PMID: 17947507] (Flow, Human) Flow Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol NCR2
Entrez
Uniprot