Recombinant Human/Mouse/Rat BMP-2 Protein, CF

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Recombinant human/mouse/rat BMP-2 (355-BM/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 40-200 ng/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human/Mouse/Rat BMP-2 Protein, CF Summary

Additional Information
More economical E. coli option available
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Gln283-Arg396
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15-16 kDa, reducing conditions
Publications
Read Publications using
355-BM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCI.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat BMP-2 Protein, CF

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2
  • SSFSC

Background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

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Publications for BMP-2 (355-BM/CF)(180)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, N/A, Phallusia mammillata, Porcine, Rabbit, Rats, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 10 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA Capture, In Ovo, In Vivo, In vivo, Organoid Culture, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(2)
Bioassay
(148)
Cell Culture
(8)
ELISA (Capture)
(1)
ELISA Capture
(1)
In Ovo
(1)
In Vivo
(10)
In vivo
(1)
Organoid Culture
(1)
Surface Plasmon Resonance
(5)
All Applications
Filter By Species
Human
(75)
Mouse
(74)
Rat
(9)
Bovine
(3)
Canine
(1)
Chicken
(4)
Equine
(1)
N/A
(1)
Phallusia mammillata
(1)
Porcine
(2)
Rabbit
(2)
Rats
(1)
Transgenic Mouse
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 180. Show All 180 Publications.
Publications using 355-BM/CF Applications Species
Y Hachinohe, M Taira, M Hoshi, W Hatakeyama, T Sawada, H Kondo Bone Formation on Murine Cranial Bone by Injectable Cross-Linked Hyaluronic Acid Containing Nano-Hydroxyapatite and Bone Morphogenetic Protein Polymers, 2022;14(24):. 2022 [PMID: 36559734] (In Vivo, Mouse) In Vivo Mouse
Z Shonibare, M Monavarian, K O'Connell, D Altomare, A Shelton, S Mehta, R Jaskula-Sz, R Phaeton, MD Starr, R Whitaker, A Berchuck, AB Nixon, RC Arend, NY Lee, CR Miller, N Hempel, K Mythreye Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer Oncogene, 2022;40(4):111066. 2022 [PMID: 35905726] (Bioassay, Human) Bioassay Human
R Chowdhury, A Roure, Y le Pétillo, H Mayeur, V Daric, S Darras Highly distinct genetic programs for peripheral nervous system formation in chordates Bmc Biology, 2022;20(1):152. 2022 [PMID: 35761237] (Bioassay, Phallusia mammillata) Bioassay Phallusia mammillata
M Vora, A Mondal, D Jia, P Gaddipati, M Akel, J Gilleran, J Roberge, C Rongo, J Langenfeld Bone morphogenetic protein signaling regulation of AMPK and PI3K in lung cancer cells and C. elegans Cell & bioscience, 2022;12(1):76. 2022 [PMID: 35641992] (Bioassay, Human) Bioassay Human
AP Leinroth, AJ Mirando, D Rouse, Y Kobayahsi, PR Tata, HE Rueckert, Y Liao, JT Long, JV Chakkalaka, MJ Hilton Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations Cell Reports, 2022;39(6):110785. 2022 [PMID: 35545045] (Bioassay, Mouse) Bioassay Mouse
F Kawecki, J Jann, M Fortin, FA Auger, N Faucheux, J Fradette Preclinical Evaluation of BMP-9-Treated Human Bone-like Substitutes for Alveolar Ridge Preservation following Tooth Extraction International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328724] (Cell Culture, Human) Cell Culture Human
S Nagel, C Pommerenke, C Meyer, RAF MacLeod The Hematopoietic TALE-Code Shows Normal Activity of IRX1 in Myeloid Progenitors and Reveals Ectopic Expression of IRX3 and IRX5 in Acute Myeloid Leukemia International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328612] (Cell Culture, Human) Cell Culture Human
I López-Anto, C Contreras-, L Luque-Mart, A Carpintero, P González-M, I Palmero Dynamic regulation of myofibroblast phenotype in cellular senescence Aging Cell, 2022;0(0):e13580. 2022 [PMID: 35266275] (Bioassay, Human) Bioassay Human
M Moncayo-Do, GA Rico-Llano, DA Garzón-Alv, J Becerra, R Visser, MR Fontanilla The Effect of Pore Directionality of Collagen Scaffolds on Cell Differentiation and In Vivo Osteogenesis Polymers, 2021;13(18):. 2021 [PMID: 34578088] (In Vivo, Rats) In Vivo Rats
M Zheng, S Erhardt, D Ai, J Wang Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development International Journal of Molecular Sciences, 2021;22(18):. 2021 [PMID: 34576009] (Bioassay, Mouse) Bioassay Mouse
Show All 180 Publications.

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FAQs for BMP-2 (355-BM/CF). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Bioinformatics

Gene Symbol BMP2
Uniprot