Recombinant Human/Mouse/Rat BMP-2 Protein, CF


Recombinant human/mouse/rat BMP-2 (355-BM/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 40-200 ng/mL.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human/Mouse/Rat BMP-2 Protein, CF Summary

Additional Information
More economical E. coli option available
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
The specific activity of Recombinant Human BMP-2 is approximately 781 U/μg, which is calibrated against human BMP-2 WHO International Standard (NIBSC code: 93/574).   Specific activity is for reference purposes only and is not routinely tested.  
Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
15-16 kDa, reducing conditions
Read Publications using
355-BM/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCI.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat BMP-2 Protein, CF

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2


Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

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Publications for BMP-2 (355-BM/CF)(162)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, N/A, Porcine, Rabbit, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 10 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA Capture, In Ovo, In Vivo, In vivo, Organoid Culture, Surface Plasmon Resonance.

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Showing Publications 1 - 10 of 162. Show All 162 Publications.
Publications using 355-BM/CF Applications Species
PS Briquez, HM Tsai, EA Watkins, JA Hubbell Engineered bridge protein with dual affinity for bone morphogenetic protein-2 and collagen enhances bone regeneration for spinal fusion Science Advances, 2021;7(24):. 2021 [PMID: 34117071] (ELISA Capture, Human) ELISA Capture Human
K Hiraoka, H Inada, K Yanai, N Osumi Bone Morphogenetic Proteins Inhibit Ciliogenesis of Ependymal Cells in Vitro Tohoku J Exp Med, 2020;252(3):199-208. 2020 [PMID: 33087680] (Bioassay, Mouse) Bioassay Mouse
T Mang, K Kleinschmi, F Ploeger, A Schoeneman, S Lindemann, A Gigout BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation J. Cell. Sci., 2020;0(0):. 2020 [PMID: 32764110] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
SK Meurer, O Tezcan, T Lammers, R Weiskirche Differential regulation of Lipocalin 2 (LCN2) in doxorubicin-resistant 4T1 triple negative breast cancer cells Cell. Signal., 2020;74(0):109731. 2020 [PMID: 32758668] (Bioassay, Mouse) Bioassay Mouse
L Meng, X Teng, Y Liu, C Yang, S Wang, W Yuan, J Meng, H Chi, L Duan, X Liu Vital Roles of Gremlin-1 in Pulmonary Arterial Hypertension Induced by Systemic-to-Pulmonary Shunts J Am Heart Assoc, 2020;9(15):e016586. 2020 [PMID: 32750294] (Bioassay, Rat) Bioassay Rat
AS Ibrahim, K Hussein, F Wang, M Wan, N Saad, M Essa, I Kim, A Shakoor, LA Owen, MM DeAngelis, M Al-Shabraw Bone Morphogenetic Protein (BMP)4 But Not BMP2 Disrupts the Barrier Integrity of Retinal Pigment Epithelia and Induces Their Migration: A Potential Role in Neovascular Age-Related Macular Degeneration J Clin Med, 2020;9(7):. 2020 [PMID: 32707711] (Bioassay, Human) Bioassay Human
A VanCleave, M Palmer, F Fang, H Torres, T Rodezno, Q Li, K Fuglsby, C Evans, Y Afeworki, A Ross, P Rao, P Leiferman, S Zheng, P Houghton, J Tao Development and characterization of the novel human osteosarcoma cell line COS-33 with sustained activation of the mTOR pathway Oncotarget, 2020;11(27):2597-2610. 2020 [PMID: 32676162] (Bioassay, Human) Bioassay Human
S Qin, W Wang, Z Liu, X Hua, S Fu, F Dong, A Li, Z Liu, P Wang, L Dai, P Liang, J Zhang, W Cao, X Xiong, H Chen, J Xu Fibrochondrogenic differentiation potential of tendon-derived stem/progenitor cells from human patellar tendon J Orthop Translat, 2020;22(0):101-108. 2020 [PMID: 32440505] (Cell Culture, Human) Cell Culture Human
A Dudakovic, RM Samsonraj, CR Paradise, C Galeano-Ga, MO Mol, D Galeano-Ga, P Zan, ML Galvan, M Hevesi, O Pichurin, R Thaler, DL Begun, P Kloen, M Karperien, AN Larson, JJ Westendorf, SM Cool, AJ van Wijnen Inhibition of the epigenetic suppressor EZH2 primes osteogenic differentiation mediated by BMP2 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32332097] (Bioassay, Mouse) Bioassay Mouse
A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration Int J Mol Sci, 2020;21(8):. 2020 [PMID: 32295299] (Bioassay, Human) Bioassay Human
Show All 162 Publications.

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FAQs for BMP-2 (355-BM/CF). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Gene Symbol BMP2