Recombinant Human/Mouse/Rat BMP-2 Protein, CF

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human/Mouse/Rat BMP-2 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
The specific activity of Recombinant Human BMP-2 is approximately 781 U/μg, which is calibrated against human BMP-2 WHO International Standard (NIBSC code: 93/574).
Source
Chinese Hamster Ovary cell line, CHO-derived BMP-2 protein
Gln283-Arg396
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15-16 kDa, reducing conditions
Publications
Read Publications using
355-BM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCI.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat BMP-2 Protein, CF

  • BDA2
  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2
  • SSFSC

Background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP‑2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR‑IA, or BMPR‑IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

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355-BM/CF
Species: Hu, Mu, Rt
Applications: Bioactivity

Publications for BMP-2 (355-BM/CF)(139)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Porcine, Rabbit, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 8 applications: Binding Assay, Bioassay, ELISA (Capture), In Ovo, In Vivo, In vivo, Organoid Culture, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(2)
Bioassay
(122)
ELISA (Capture)
(1)
In Ovo
(1)
In Vivo
(7)
In vivo
(1)
Organoid Culture
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(49)
Mouse
(64)
Rat
(7)
Bovine
(3)
Canine
(1)
Chicken
(4)
Equine
(1)
Porcine
(2)
Rabbit
(2)
Transgenic Mouse
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 139. Show All 139 Publications.
Publications using 355-BM/CF Applications Species
M Rosina, F Langone, G Giuliani, A Cerquone P, A Reggio, A Calderone, C Fuoco, L Castagnoli, C Gargioli, G Cesareni Osteogenic differentiation of skeletal muscle progenitor cells is activated by the DNA damage response Sci Rep, 2019;9(1):5447. 2019 [PMID: 30931986] (Bioassay, Mouse) Bioassay Mouse
X Zhang, X Lin, T Liu, L Deng, Y Huang, Y Liu Osteogenic Enhancement Between Icariin and Bone Morphogenetic Protein 2: A Potential Osteogenic Compound for Bone Tissue Engineering Front Pharmacol, 2019;10(0):201. 2019 [PMID: 30914948] (Bioassay, Human) Bioassay Human
HJ Kim, HJ Kim, Y Choi, MK Bae, DS Hwang, SH Shin, JY Lee Zoledronate Enhances Osteocyte-Mediated Osteoclast Differentiation by IL-6/RANKL Axis Int J Mol Sci, 2019;20(6):. 2019 [PMID: 30909508] (Bioassay, Mouse) Bioassay Mouse
S Supakul, K Yao, H Ochi, T Shimada, K Hashimoto, S Sunamura, Y Mabuchi, M Tanaka, C Akazawa, T Nakamura, A Okawa, S Takeda, S Sato Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing Int J Mol Sci, 2019;20(5):. 2019 [PMID: 30832329] (Bioassay, Mouse) Bioassay Mouse
WKW Ho, L Freem, D Zhao, KJ Painter, TE Woolley, EA Gaffney, MJ McGrew, A Tzika, MC Milinkovit, P Schneider, A Drusko, F Matthäus, JD Glover, KL Wells, JA Johansson, MG Davey, HM Sang, M Clinton, DJ Headon Feather arrays are patterned by interacting signalling and cell density waves PLoS Biol., 2019;17(2):e3000132. 2019 [PMID: 30789897] (Bioassay, Chicken) Bioassay Chicken
L Yu, LA Dawson, M Yan, K Zimmel, YL Lin, CP Dolan, M Han, K Muneoka BMP9 stimulates joint regeneration at digit amputation wounds in mice Nat Commun, 2019;10(1):424. 2019 [PMID: 30723209] (In Vivo, Mouse) In Vivo Mouse
T Karakida, K Onuma, MM Saito, R Yamamoto, T Chiba, R Chiba, Y Hidaka, K Fujii-Abe, H Kawahara, Y Yamakoshi Potential for Drug Repositioning of Midazolam for Dentin Regeneration Int J Mol Sci, 2019;20(3):. 2019 [PMID: 30720745] (Bioassay, Porcine) Bioassay Porcine
S Hendrikx, S Coso, B Prat-Luri, L Wetterwald, A Sabine, CA Franco, S Nassiri, N Zangger, H Gerhardt, M Delorenzi, TV Petrova Endothelial Calcineurin Signaling Restrains Metastatic Outgrowth by Regulating Bmp2 Cell Rep, 2019;26(5):1227-1241.e6. 2019 [PMID: 30699351] (Bioassay, Mouse) Bioassay Mouse
WN Addison, KC Hall, S Kokabu, T Matsubara, MM Fu, F Gori, R Baron Zfp423 Regulates Skeletal Muscle Regeneration and Proliferation Mol. Cell. Biol., 2019;0(0):. 2019 [PMID: 30692273] (Bioassay, Human) Bioassay Human
M Subileau, G Merdzhanov, D Ciais, V Collin-Fau, JJ Feige, S Bailly, D Vittet Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation Stem Cell Reports, 2018;0(0):. 2018 [PMID: 30595547] (Bioassay, Mouse) Bioassay Mouse
Show All 139 Publications.

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FAQs for BMP-2 (355-BM/CF). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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Bioinformatics

Gene Symbol BMP2
Uniprot