Recombinant Human Lumican Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Lumican Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. When 5 x 104 cells per well are added to Recombinant Human Lumican coated plates (10 μg/mL, 100 μL/well),  >35% will adhere after 50 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application. Also measured by its ability to modulate collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived human Lumican protein
Gln19-Asn338, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln19 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
LUM
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-65 kDa, reducing conditions
Publications
Read Publications using
2846-LU in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lumican Protein, CF

  • KSPG lumican
  • LDC
  • LUM
  • lumican proteoglycan
  • Lumican
  • SLRR2D
  • SLRR2DLDCKeratan sulfate proteoglycan lumican

Background

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Human Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain (SwissProt #: P51884). The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature human Lumican is 88%, 87%, and 70% aa identical to mature mouse, rat, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers
(1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8, 9), and adhesion (9). Lumican’s over-expression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).
  1. Nikitovic, D. et al. (2008) IUBMB Life 60:818.
  2. Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
  3. Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
  4. Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
  5. Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
  6. Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
  7. Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294.
  8. Nikitovic, D. et al. (2008) FEBS J. 275:350.
  9. D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
  10. Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.

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Publications for Lumican (2846-LU)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: Bioassay, Enzyme Assay.


Filter By Application
Bioassay
(5)
Enzyme Assay
(1)
All Applications
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Human
(5)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 2846-LU Applications Species
X Li, Y Kang, D Roife, Y Lee, M Pratt, MR Perez, B Dai, EJ Koay, JB Fleming Prolonged exposure to extracellular lumican restrains pancreatic adenocarcinoma growth Oncogene, 2017;0(0):. 2017 [PMID: 28534517] (Bioassay, Human) Bioassay Human
K Karamanou, M Franchi, Z Piperigkou, C Perreau, FX Maquart, DH Vynios, S Brézillon Lumican effectively regulates the estrogen receptors-associated functional properties of breast cancer cells, expression of matrix effectors and epithelial-to-mesenchymal transition Sci Rep, 2017;7(0):45138. 2017 [PMID: 28332606] (Bioassay, Human) Bioassay Human
Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors Clin Cancer Res, 2016;0(0):. 2016 [PMID: 27126993] (Bioassay, Human) Bioassay Human
Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Invest Ophthalmol Vis Sci, 2013;54(2):1014-25. 2013 [PMID: 23322569] (Enzyme Assay, Human) Enzyme Assay Human
Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis. Lab Invest, 2012;92(12):1712-25. 2012 [PMID: 23007134] (Bioassay, Rat) Bioassay Rat
Seomun Y, Joo CK, Lumican induces human corneal epithelial cell migration and integrin expression via ERK 1/2 signaling. Biochem. Biophys. Res. Commun., 2008;372(1):221-5. 2008 [PMID: 18477477] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol LUM
Uniprot