Recombinant Human LIGHT/TNFSF14 Protein, CF


Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.

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Recombinant Human LIGHT/TNFSF14 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.3-3 ng/mL.
Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein
Met 10-His tag GGGSGGGSGGGSIEGR Human Light
Accession # O43557
N-terminus C-terminus
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity2
Theoretical MW
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
25 kDa, reducing conditions
Read Publications using
664-LI/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LIGHT/TNFSF14 Protein, CF

  • CD258 antigen
  • CD258
  • delta transmembrane LIGHT
  • Herpes virus entry mediator ligand
  • Herpesvirus entry mediator ligand
  • HVEM-L
  • HVEMLherpesvirus entry mediator-ligand
  • ligand for herpesvirus entry mediator
  • LIGHTherpesvirus entry mediator A
  • LTg
  • TNFSF14
  • TR2
  • tumor necrosis factor (ligand) superfamily, member 14
  • tumor necrosis factor ligand superfamily member 14
  • tumor necrosis factor receptor-like 2
  • tumor necrosis factor superfamily member LIGHT


Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma . Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma . The full roles of LIGHT remain to be elucidated.

  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Publications for LIGHT/TNFSF14 (664-LI/CF)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, Surface Plasmon Resonance (SPR.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 664-LI/CF Applications Species
SK Heo, Y Choi, YK Jeong, LJ Ju, HM Yu, DK Kim, HJ Seo, YJ Lee, J Cheon, S Koh, YJ Min, EK Noh, JC Jo LIGHT (TNFSF14) enhances osteogenesis of human bone marrow-derived mesenchymal stem cells PLoS ONE, 2021-02-19;16(2):e0247368. 2021-02-19 [PMID: 33606781] (Bioassay, Human) Bioassay Human
Jang H, Park S, Kim J, Kim J, Kim S, Cho S, Park S, Park B, Kim S, Kim J The Tumor Suppressor, p53, Negatively Regulates Non-Canonical NF-kappaB Signaling through miRNAInduced Silencing of NF-kappaB-Inducing Kinase. Mol Cells, 2020-01-31;43(1):23-33. 2020-01-31 [PMID: 31870133] (Bioassay, Human) Bioassay Human
S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf, 2019-04-06;0(0):. 2019-04-06 [PMID: 30965123] (Bioassay, Human) Bioassay Human
KH Kim, H Choi, HJ Kim, TR Lee TNFSF14 inhibits melanogenesis via NF-kB signaling in melanocytes Cytokine, 2018-05-03;110(0):126-130. 2018-05-03 [PMID: 29730385] (Bioassay, Human) Bioassay Human
R da Silva A, AK Mehta, L Madge, J Tocker, M Croft TNFSF14 (LIGHT) Exhibits Inflammatory Activities in Lung Fibroblasts Complementary to IL-13 and TGF-β Front Immunol, 2018-03-19;9(0):576. 2018-03-19 [PMID: 29616048] (Cell Culture, Human) Cell Culture Human
AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-03-05;0(0):1-43. 2018-03-05 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
S Abadpour, B Halvorsen, A Sahraoui, O Korsgren, P Aukrust, H Scholz Interleukin-22 reverses human islet dysfunction and apoptosis triggered by hyperglycemia and LIGHT J. Mol. Endocrinol., 2018-01-12;0(0):. 2018-01-12 [PMID: 29330151] (Bioassay, Human) Bioassay Human
X Yuan, Y Gu, X Lai, Q Gu LIGHT is increased in patients with coronary disease and regulates inflammatory response and lipid metabolism in oxLDL-induced THP-1 macrophages Biochem. Biophys. Res. Commun., 2017-06-19;0(0):. 2017-06-19 [PMID: 28642135] (Bioassay, Human) Bioassay Human
A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice Nat Commun, 2016-12-16;7(0):13696. 2016-12-16 [PMID: 27982078] (Bioassay, Human) Bioassay Human
LIGHT (TNFSF14) Increases the Survival and Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells PLoS ONE, 2016-11-11;11(11):e0166589. 2016-11-11 [PMID: 27835685] (Bioassay, Human) Bioassay Human
Show All 18 Publications.

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Gene Symbol TNFSF14