Recombinant Human LIGHT/TNFSF14 Protein, CF

Images

 
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA, BA2
Format
Carrier-Free

Order Details

Recombinant Human LIGHT/TNFSF14 Protein, CF Summary

Details of Functionality
Measured in a cytotoxicity assay using HT‑29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142. Recombinant Human LIGHT/TNFSF14 at 10 ng/mL causes a 2-4.5 fold inhibition of HT29 proliferation, in the presence of 10 U/mL (1 ng/mL) Recombinant Human IFN-gamma (Catalog # 285-IF). Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Met 10-His tag GGGSGGGSGGGSIEGR Human Light
(Asp74-Val240)
Accession # O43557
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
TNFSF14
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
25 kDa, reducing conditions
Publications
Read Publications using
664-LI/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LIGHT/TNFSF14 Protein, CF

  • CD258 antigen
  • CD258
  • delta transmembrane LIGHT
  • Herpes virus entry mediator ligand
  • Herpesvirus entry mediator ligand
  • HVEM-L
  • HVEMLherpesvirus entry mediator-ligand
  • ligand for herpesvirus entry mediator
  • LIGHT
  • LIGHTherpesvirus entry mediator A
  • LTg
  • TNFSF14
  • TR2
  • tumor necrosis factor (ligand) superfamily, member 14
  • tumor necrosis factor ligand superfamily member 14
  • tumor necrosis factor receptor-like 2
  • tumor necrosis factor superfamily member LIGHT

Background

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.

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Publications for (10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(10)
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Human
(10)
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Showing Publications 1 - 10 of 10.
Publications using 664-LI/CF Applications Species
A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice Nat Commun, 2016;7(0):13696. 2016 [PMID: 27982078] (Bioassay, Human) Bioassay Human
LIGHT (TNFSF14) Increases the Survival and Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells PLoS ONE, 2016;11(11):e0166589. 2016 [PMID: 27835685] (Bioassay, Human) Bioassay Human
da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells. J Immunol, 2015;195(5):2429-41. 2015 [PMID: 26209626] (Bioassay, Human) Bioassay Human
Herro R, Antunes R, Aguilera A, Tamada K, Croft M The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis. J Invest Dermatol, 2015;135(8):2109-18. 2015 [PMID: 25789702] (Bioassay, Human) Bioassay Human
Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, Kohyama T, Takizawa H, Nagase T, Yamauchi Y Lymphotoxin beta receptor signaling induces IL-8 production in human bronchial epithelial cells. PLoS ONE, 2014;9(12):e114791. 2014 [PMID: 25501580] (Bioassay, Human) Bioassay Human
Jin HR, Jin X, Lee JJ Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway. Biochem. Biophys. Res. Commun., 2010;400(4):581-6. 2010 [PMID: 20804734] (Bioassay, Human) Bioassay Human
Sandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis. Circ. Res., 2009;104(1):60-8. 2009 [PMID: 19023130] (Bioassay, Human) Bioassay Human
Kang YM, Kim SY, Kang JH LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes. Arthritis Rheum., 2007;56(4):1106-17. 2007 [PMID: 17393389] (Bioassay, Human) Bioassay Human
Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006;108(3):928-35. 2006 [PMID: 16861346] (Bioassay, Human) Bioassay Human
Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Arthritis Rheum., 2006;54(5):1451-62. 2006 [PMID: 16649193] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol TNFSF14
Entrez
Uniprot