Recombinant Human LBP Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human LBP Protein Summary

• Details of Functionality: Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.25‑2.5 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human LBP protein
  Ala26-Val481
• Accession #: AAD21962
• N-terminal Sequence: Ala26
• Protein/Peptide Type: Recombinant Proteins
• Gene: LBP
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LBP Protein

• LBP
• lipopolysaccharide binding protein
• lipopolysaccharide-binding protein
• LPS-binding protein
• MGC22233

Background

Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.

1. Hailman, E. et al. (1994) J. Exp. Med. 179:269.
2. Jack, R.S. et al. (1997) Nature 389:742.
3. Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.

Publications for LBP (870-LP)(16)

Publications using 870-LP

• Hayashi, K;Sano, M;Kanayasu-Toyoda, T;Morita, Y;Yamaguchi, T;Ohya, K;Kikuchi, Y;Izutsu, KI;Hara-Kudo, Y; Evaluation of the effect of cell freshness on pyrogen detection using a serum-free monocyte-activation test PloS one 2024-12-30 [PMID: 39775542] (Bioassay, Human)
• Lin, YH;Kuo, NR;Shen, HC;Chang, YC;Lin, R;Liao, TL;Yeh, HY;Yang, YY;Hou, MC;Lin, HC; Prediction models combining zonulin, LPS, and LBP predict acute kidney injury and hepatorenal syndrome-acute kidney injury in cirrhotic patients Scientific reports 2023-08-11 [PMID: 37567912] (Bioassay, Human)
• A Pérez-Pons, M Jara-Aceve, A Henriques, P Navarro-Na, AC García-Mon, I Álvarez-Tw, CE Pedreira, L Sánchez-Mu, D Damasceno, C Caldas, JI Muñoz-Gonz, A Matito, J Flores-Mon, O González-L, I Criado, A Mayado, A Orfao Altered innate immune profile in blood of systemic mastocytosis patients Clinical and translational allergy, 2022-06-14;12(6):e12167. 2022-06-14 [PMID: 35734269] (Bioassay, Human)
• A Molinaro, A Koh, H Wu, M Schoeler, I Faggi, A Carreras, A Hallén, F Bäckhed, R Caesar Hepatic expression of Lipopolysaccharide Binding Protein (Lbp) is induced by the gut microbiota through Myd88 and impairs glucose tolerance in mice independent of obesity Mol Metab, 2020-04-16;0(0):100997. 2020-04-16 [PMID: 32305515] (Bioassay, Mouse)
• AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming Cell Rep, 2019-11-19;29(8):2505-2519.e4. 2019-11-19 [PMID: 31747616] (Bioassay, Human)
• SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh Tie2 protects the vasculature against thrombus formation in systemic inflammation J. Clin. Invest., 2018-03-05;0(0):. 2018-03-05 [PMID: 29360642] (Bioassay, Human)
• R Lehmann, MM Müller, TE Klassert, D Driesch, M Stock, A Heinrich, T Conrad, C Moore, UK Schier, R Guthke, H Slevogt Differential regulation of the transcriptomic and secretomic landscape of sensor and effector functions of human airway epithelial cells Mucosal Immunol, 2018-01-03;0(0):. 2018-01-03 [PMID: 29297499] (Bioassay, Human)
• PH Ding, RP Darveau, CY Wang, L Jin 3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes PLoS ONE, 2017-04-06;12(4):e0173223. 2017-04-06 [PMID: 28384159] (Bioassay, Human)
• B S Lashkari, DO Anumba Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4 PLoS ONE, 2017-03-15;12(3):e0173646. 2017-03-15 [PMID: 28296959] (Bioassay, Human)
• Annette C Wensink Granzymes A and K differentially potentiate LPS-induced cytokine response Cell Death Discov, 2016-12-12;2(0):16084. 2016-12-12 [PMID: 28028441] (Bioassay)
• Fang L, Xu Z, Wang G, Ji F, Mei C, Liu J, Wu G Directed evolution of an LBP/CD14 inhibitory peptide and its anti-endotoxin activity. PLoS ONE, 2014-07-15;9(7):e101406. 2014-07-15 [PMID: 25025695] (Bioassay, Enzyme Assay, Human)
• Canault M, Ghalloussi D, Grosdidier C, Guinier M, Perret C, Chelghoum N, Germain M, Raslova H, Peiretti F, Morange P, Saut N, Pillois X, Nurden A, Cambien F, Pierres A, van den Berg T, Kuijpers T, Alessi M, Tregouet D Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding. J Exp Med, 2014-06-23;211(7):1349-62. 2014-06-23 [PMID: 24958846] (Bioassay, Human)
• Cordeiro , Cristian, Esmaili , Hamed, Ansah , George, Hincke , Maxwell Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes. PLoS ONE, 2013-12-31;8(12):e84112. 2013-12-31 [PMID: 24391897] (Bioassay, Chicken)
• Malachowa N, Kobayashi S, Freedman B, Dorward D, DeLeo F Staphylococcus aureus leukotoxin GH promotes formation of neutrophil extracellular traps. J Immunol, 2013-11-04;191(12):6022-9. 2013-11-04 [PMID: 24190656] (Bioassay, Human)
• Gupta N, Li W, Willard B, Silverstein R, McIntyre T Proteasome proteolysis supports stimulated platelet function and thrombosis. Arterioscler Thromb Vasc Biol, 2013-10-31;34(1):160-8. 2013-10-31 [PMID: 24177323] (Bioassay, Human)
• Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding. PLoS ONE, 2011-08-01;6(8):e23132. 2011-08-01 [PMID: 21829704] (Bioassay, Human)

Bioinformatics

• Gene Symbol: LBP
• Uniprot:
  • AAD21962()