Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. When 5 x 104 cells/well are added to Recombinant Human L‑Selectin/CD62L Fc Chimera coated plates, adhesion is induced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.4-2 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human L-Selectin/CD62L protein
Human L-Selectin
(Trp39 - Asn332)
Accession # P14151
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Trp39
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SELL
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
60.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-100 kDa, reducing conditions
Publications
Read Publications using
728-LS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 0.1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human L-Selectin/CD62L Fc Chimera Protein, CF

  • CD62L antigen
  • CD62L
  • gp90-MEL
  • hLHRc
  • LAM1
  • LAM-1
  • LAM1LECAM1
  • LECAM1
  • LEU8
  • Leu-8
  • Leukocyte adhesion molecule 1
  • Leukocyte surface antigen Leu-8
  • Leukocyte-endothelial cell adhesion molecule 1
  • LNHR
  • LNHRTQ1
  • LSEL
  • L-Selectin
  • LYAM1
  • Lyam-1
  • LYAM1CD62 antigen-like family member L
  • Lymph node homing receptor
  • lymphocyte adhesion molecule 1
  • pln homing receptor
  • PLNHR
  • selectin L
  • SELL
  • TQ1

Background

L-Selectin (also known as Leukocyte Selectin, LAM‑1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L‑Selectin share 76% amino acid (aa) sequence homology.

L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction, also involving ICAM-1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

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728-LS
Species: Hu
Applications: Bioactivity

Publications for L-Selectin/CD62L (728-LS)(9)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, Western Blot (Detection).


Filter By Application
Bioassay
(7)
Flow Cytometry
(1)
Western Blot (Detection)
(1)
All Applications
Filter By Species
Human
(6)
N/A
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using 728-LS Applications Species
GE Carlson, EW Martin, VS Shirure, R Malgor, VA Resto, DJ Goetz, MM Burdick Dynamic biochemical tissue analysis detects functional L-selectin ligands on colon cancer tissues PLoS ONE, 2017;12(3):e0173747. 2017 [PMID: 28282455] (Bioassay, N/A) Bioassay N/A
Bo Li Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases Adv Healthc Mater, 2016;0(0):. 2016 [PMID: 27943662] (Bioassay) Bioassay
Simon N Robinson Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation Cytotherapy, 2016;0(0):. 2016 [PMID: 27919572] (Bioassay, Human) Bioassay Human
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016;6(0):37953. 2016 [PMID: 27892504] (Bioassay, N/A) Bioassay N/A
Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion Sci Rep, 2016;6(0):30392. 2016 [PMID: 27458028] (Bioassay, Human) Bioassay Human
Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun, 2015;6(0):6387. 2015 [PMID: 25824568] (Bioassay, Human) Bioassay Human
Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling. J Immunol, 2014;192(4):1862-9. 2014 [PMID: 24431230] (Flow Cytometry, Human) Flow Cytometry Human
Gakhar, Gunjan, Navarro, Vicente, Jurish, Madelyn, Lee, Guang Yu, Tagawa, Scott T, Akhtar, Naveed H, Seandel, Marco, Geng, Yue, Liu, He, Bander, Neil H, Giannakakou, Paraskev, Christos, Paul J, King, Michael, Nanus, David M Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow. PLoS ONE, 2013;8(12):e85143. 2013 [PMID: 24386459] (Bioassay, Human) Bioassay Human
Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta. Dev. Biol., 2006;298(1):107-17. 2006 [PMID: 16930583] (Western Blot (Detection), Human) Western Blot (Detection) Human

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L-selectin is a member of the selectin family of glycoprotein adhesion and homing receptors that recognize sialyated carbohydrate groups and regulate lymphocyte-endothelial cell interactions. It is a type I transmembrane cell adhesion molecule (CAM) a...  Read full blog post.

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Bioinformatics

Gene Symbol SELL
Uniprot