Recombinant Human KGF/FGF-7 Protein, CF

Images

 
Recombinant Human KGF/FGF-7 (Catalog # 251-KG/CF) stimulates cell proliferation of 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 6‑60 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations

Recombinant Human KGF/FGF-7 Protein, CF Summary

Additional Information
Earn double rewards for reviews
Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 6-60 ng/mL.
The specific activity of recombinant human KGF/FGF-7 is approximately 1.3 x 103 U/μg, which is calibrated against recombinant human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FGF7
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
251-KG/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Protein, CF

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

Customers Who Viewed This Item Also Viewed...

345-FG/CF
Species: Hu
Applications: BA
NBP2-00932
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
233-FB/CF
Species: Hu
Applications: BA
294-HG/CF
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
232-FA/CF
Species: Hu
Applications: BA
NB110-68800
Species: Hu, Mu
Applications: ICC/IF, WB
DVE00
Species: Hu
Applications: ELISA
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
255-SC/CF
Species: Hu
Applications: BA
239-A
Species: Hu
Applications: BA
NBP2-35009
Species: Hu
Applications: Func, PAGE, WB

Publications for KGF/FGF-7 (251-KG/CF)(69)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Fish - Prionace glauca (Blue Shark), Hamster.

We have publications tested in 3 applications: Bioassay, Cell Culture, Stimulation.


Filter By Application
Bioassay
(61)
Cell Culture
(4)
Stimulation
(1)
All Applications
Filter By Species
Human
(54)
Mouse
(10)
Rat
(1)
Fish - Prionace glauca (Blue Shark)
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using 251-KG/CF Applications Species
R Suenaga, S Konagaya, J Yamaura, R Ito, S Tanaka, Y Ishizaki, T Toyoda Microwell bag culture for large-scale production of homogeneous islet-like clusters Scientific Reports, 2022;12(1):5221. 2022 [PMID: 35338209] (Bioassay, Human) Bioassay Human
AM Cujba, ME Alvarez-Fa, S Pedraza-Ar, A Laddach, MH Shepherd, AT Hattersley, FM Watt, R Sancho An HNF1alpha truncation associated with maturity-onset diabetes of the young impairs pancreatic progenitor differentiation by antagonizing HNF1beta function Cell Reports, 2022;38(9):110425. 2022 [PMID: 35235779] (Bioassay, Human) Bioassay Human
K Zhao, Y Shi, J Yu, L Yu, A Mael, Y Li, A Kolton, T Joyce, J Odorico, PO Berggren, SN Yang Intracameral Microimaging of Maturation of Human iPSC Derivatives into Islet Endocrine Cells Cell Transplantation, 2022;31(0):9636897211066. 2022 [PMID: 35156411] (Bioassay, Human) Bioassay Human
JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells Nature Cell Biology, 2021;0(0):. 2021 [PMID: 34969962] (Bioassay, Human) Bioassay Human
RJ Geusz, A Wang, DK Lam, NK Vinckier, KD Alysandrat, DA Roberts, J Wang, S Kefalopoul, A Ramirez, Y Qiu, J Chiou, KJ Gaulton, B Ren, DN Kotton, M Sander Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors Nature Communications, 2021;12(1):6636. 2021 [PMID: 34789735] (Bioassay, Human) Bioassay Human
DA Newton, RG Lottes, RM Ryan, DD Spyropoulo, JE Baatz Dysfunctional lactate metabolism in human alveolar type II cells from idiopathic pulmonary fibrosis lung explant tissue Respiratory Research, 2021;22(1):278. 2021 [PMID: 34711218] (Cell Culture, Human) Cell Culture Human
AV Parent, G Faleo, J Chavez, M Saxton, DI Berrios, NR Kerper, Q Tang, M Hebrok Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection Cell Reports, 2021;36(7):109538. 2021 [PMID: 34407395] (Bioassay, Human) Bioassay Human
MO Lee, SG Lee, CR Jung, YS Son, JW Ryu, KB Jung, JH Ahn, JH Oh, HA Lee, JH Lim, J Kim, I Jang, J Choi, J Jung, K Park, B Lee, DS Kim, MY Son, HS Cho Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells Nature Communications, 2021;12(1):4492. 2021 [PMID: 34301945] (Bioassay, Human) Bioassay Human
AC Harttrampf, MEM da Costa, A Renoult, E Daudigeos-, B Geoerger Histone deacetylase inhibitor panobinostat induces antitumor activity in epithelioid sarcoma and rhabdoid tumor by growth factor receptor modulation BMC Cancer, 2021;21(1):833. 2021 [PMID: 34281526] (Stimulation, Human) Stimulation Human
AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D Nature Protocols, 2021;0(0):. 2021 [PMID: 33649566] (Bioassay, Human) Bioassay Human
Show All 69 Publications.

Reviews for KGF/FGF-7 (251-KG/CF) (0)

There are no reviews for KGF/FGF-7 (251-KG/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for KGF/FGF-7 (251-KG/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human KGF/FGF-7 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF7
Uniprot