Recombinant Human KGF/FGF-7 Protein, CF

Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) induced cell proliferation in Ba/F3 mouse pro-B cells transfected with human FGF RIIb. The ED50 for this effect is 6.00-60.0 ng/mL.

2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 20 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human KGF/FGF-7 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED₅₀ for this effect is 6.00-60.0 ng/mL.
• Source: E. coli-derived human KGF/FGF-7 protein
  Cys32-Thr194, with an N-terminal Met
• Accession #: P21781.1
• N-terminal Sequence: Cys32 & Asn33
• Protein/Peptide Type: Recombinant Proteins
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 kDa, reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with Trehalose.
• Reconstitution Instructions: Reconstitute the 20 μg size at 200 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Protein, CF

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

Keratinocyte Growth Factor (KGF), or Fibroblast Growth Factor-7 (FGF-7), is an important component in many cell culture protocols. It is widely used to promote the growth and differentiation of epithelial cells, including skin keratinocytes, and lung, corneal, and intestinal epithelia. It is a part of tissue engineering and organoid culture protocols, where it can enhance the generation of complex tissues such as liver, gastrointestinal tract, lung, and mammary gland. Furthermore, KGF/FGF-7 has been shown to stimulate the proliferation of pancreatic endocrine progenitor cells and promote their differentiation into mature insulin-secreting beta cells. In these capacities KGF/FGF-7 has significant clinical relevance in studies to better understand gastrointestinal disorders, wound healing, respiratory disease, and diabetes. 

Bioinformatics

• Uniprot:
  • P21781.1()