Recombinant Human JAM-C Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human JAM-C Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes on immobilized JAM-2 (VE-JAM)/Fc Chimera. When 0.2 µg/mL (100 µL/well) of rhJAM-B/Fc Chimera is immobilized on goat anti-human IgG Fc Chimera antibody coated wells, the ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 1 x 10⁵ cells/well.
• Source: Mouse myeloma cell line, NS0-derived human JAM-C protein
  

  Human JAM-C Val32 - Asn241 (Ala149Pro) Accession # Q9BX67	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9BX67
• N-terminal Sequence: Val32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: JAM3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human JAM-C Fc Chimera Protein, CF

• CD323
• JAM-2
• JAM3
• JAMC
• JAM-C
• JAM-CFLJ14529
• junctional adhesion molecule 3JAM-3
• junctional adhesion molecule C

Background

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 46 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3, 4). Human JAM-C shares 86% aa sequence identity with its mouse homologue. It also shares approximately 36% and 32% aa sequence homology with human JAM-B and JAM-A, respectively (3 - 5). Human JAM-C shows widespread tissue expression and the highest levels are found in the placenta, brain, kidney and heart. JAM-C is expressed on endothelial cells of high endothelial venules in human tonsil. It is also expressed on platelets, T-cells and NK cells (3 - 5). Unlike other JAM family members, JAM-C forms only weak homotypic interactions. JAM-C binds to JAM-B to facilitate the interactions between JAM-B and the integrin alpha4beta1 (6). This heterotypic interaction between leukocyte JAM-C and endothelial JAM-B may play a role in regulating leukocyte transmigration (5). On platelets, JAM-C is a counter-receptor for the leukocyte integrin Mac-1(CD11b/CD18) (7). JAM-C has also been identified as a strong candidate gene for hypoplastic left heart syndrome (8).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B. Under this system, JAM-C refers to the protein encoded by the gene localized to human chromosome 11.

1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
3. Arrate, M.P. et al. (2001) J. Biol. Chem. 276:45826.
4. Liang, T. et al. (2002) J. Immunol. 168:1618.
5. Johnson-Leger, C. et al. (2002) Blood 100:25793.
6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
7. Santoso, S. et al. (2002) J. Exp. Med. 196:679.
8. Phillips, H.M. et al. (2002) Genomics 79:475.

Publications for JAM-C (1189-J3)(5)

Publications using 1189-J3

• Pareja, J;Aydin, S;Zbinden, M;Bouillet, E;Zollinger, N;Theivendram, V;Fahmi, A;Pleska?, P;Barcos, S;Paas, F;Kloster, F;Blázquez, AM;Fonta, N;Merkler, D;Deutsch, U;Engelhardt, B; Lack of junctional adhesion molecule (JAM)-B traps CD8 T cells in CNS border zones and ameliorates autoimmune neuroinflammation Acta neuropathologica communications 2025-05-26 [PMID: 40420242] (Bioassay, Mouse)
• M Czabanka, LL Petrilli, S Elvers-Hor, K Bieback, B Albert Imh, P Vajkoczy, M Vinci Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis Int J Mol Sci, 2020-02-11;21(4):. 2020-02-11 [PMID: 32054130] (Cell Culture, Human)
• D Wolf, N Anto-Miche, H Blankenbac, A Wiedemann, K Buscher, JD Hohmann, B Lim, M Bäuml, A Marki, M Mauler, D Duerschmie, Z Fan, H Winkels, D Sidler, P Diehl, DM Zajonc, I Hilgendorf, P Stachon, T Marchini, F Willecke, M Schell, B Sommer, C von Zur Mu, J Reinöhl, T Gerhardt, EF Plow, V Yakubenko, P Libby, C Bode, K Ley, K Peter, A Zirlik A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense Nat Commun, 2018-02-06;9(1):525. 2018-02-06 [PMID: 29410422] (Bioassay, Human)
• Arcangeli M, Bardin F, Frontera V, Bidaut G, Obrados E, Adams R, Chabannon C, Aurrand-Lions M Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells. Stem Cells, 2014-04-01;32(4):1043-54. 2014-04-01 [PMID: 24357068] (Bioassay, Human)
• Zen K, Babbin BA, Liu Y, Whelan JB, Nusrat A, Parkos CA JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration. Mol. Biol. Cell, 2004-06-11;15(8):3926-37. 2004-06-11 [PMID: 15194813] (Binding Assay, Human)

Bioinformatics

• Gene Symbol: JAM3
• Uniprot:
  • Q9BX67()